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Preparation method of drug-loaded vegetable protein nanoparticles

A plant protein and nanoparticle technology, applied in the direction of microcapsules, active ingredients of hydroxyl compounds, non-active ingredients of polymer compounds, etc., can solve the problems of difficult particle size control, wide particle size distribution, and long time consumption, so as to improve production efficiency , Narrow particle size distribution, flexible adjustment effect

Pending Publication Date: 2022-01-11
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. Low drug loading and difficult to control particle size
In the above traditional preparation methods, in order to obtain plant protein nanoparticles with a stable structure, not only need to add a large amount of plant protein, the amount is large, but also need to add a second polymer as a co-stabilizer, resulting in the prepared nanoparticles The drug loading is low (usually <10%), which makes it difficult to systematically control the particle size and particle size distribution of nanoparticles;
[0005] 2. The traditional preparation method is not only time-consuming, but also batch-type preparation, which is not easy to transform into large-scale continuous production

Method used

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  • Preparation method of drug-loaded vegetable protein nanoparticles
  • Preparation method of drug-loaded vegetable protein nanoparticles
  • Preparation method of drug-loaded vegetable protein nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take 0.05 g of zein and dissolve it in 50 mL of 80% (V / V) aqueous ethanol to obtain a 1 mg / mL zein solution. Get 0.1g curcumin, it is dissolved in 50mL 95% ethanol, obtains the curcumin solution of 2mg / mL. Weigh an appropriate amount of sodium citrate and citric acid, and prepare a 10 mM sodium citrate-150 μM citric acid buffer solution, so that the final pH value of the buffer solution is in the range of 7.2-7.5. The zein solution and the curcumin solution were divided into two 50mL syringes respectively, and fixed on the same syringe pump, and the flow rate of the syringe pump was set at 12mL / min. Divide the sodium citrate-citric acid buffer into two 100mL syringes and fix them on the same syringe pump, and set the flow rate of the syringe pump to 48mL / min. The four strands of material are fed with a flow rate ratio of 1:1:4:4, and the material is used to rinse the channel before feeding. After the inside of the mixer is stabilized, the product is collected at the ou...

Embodiment 2

[0032] Take 0.05 g of zein and dissolve it in 50 mL of 80% (V / V) aqueous ethanol to obtain a 1 mg / mL zein solution. Get 0.15g curcumin, it is dissolved in 50mL 95% ethanol, obtains the curcumin solution of 3mg / mL. Weigh an appropriate amount of sodium citrate and citric acid, and prepare a 10 mM sodium citrate-150 μM citric acid buffer solution, so that the final pH value of the buffer solution is in the range of 7.2-7.5. The zein solution and the curcumin solution were divided into two 50mL syringes respectively, and fixed on the same syringe pump, and the flow rate of the syringe pump was set at 12mL / min. Divide the sodium citrate-citric acid buffer into two 100mL syringes and fix them on the same syringe pump, and set the flow rate of the syringe pump to 48mL / min. The four strands of material are fed with a flow rate ratio of 1:1:4:4, and the material is used to rinse the channel before feeding. After the inside of the mixer is stabilized, the product is collected at the o...

Embodiment 3

[0034]Take 0.05 g of zein and dissolve it in 50 mL of 80% (V / V) aqueous ethanol to obtain a 1 mg / mL zein solution. Get 0.1g curcumin, it is dissolved in 50mL 95% ethanol, obtains the curcumin solution of 2mg / mL. Weigh an appropriate amount of sodium citrate and citric acid, and prepare a 10 mM sodium citrate-150 μM citric acid buffer solution, so that the final pH value of the buffer solution is in the range of 7.2-7.5. The zein solution and the curcumin solution were divided into two 50mL syringes respectively, and fixed on the same syringe pump, and the flow rate of the syringe pump was set at 12mL / min. Divide the sodium citrate-citric acid buffer solution into two 100mL syringes and fix them on the same syringe pump, and set the flow rate of the syringe pump to 72mL / min. The four strands of material are fed with a flow rate ratio of 1:1:6:6, and the material is used to rinse the channel before feeding. After the inside of the mixer is stabilized, the product is collected a...

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Abstract

The invention discloses a preparation method of drug-loaded vegetable protein nanoparticles. The preparation method comprises the following steps: dissolving vegetable protein and a natural hydrophobic drug to form a solution; preparing a sodium citrate-citric acid or phosphate buffer solution; respectively filling two injectors with the solution of vegetable protein and the natural hydrophobic drug, and fixing the injectors on the same injection pump; subpacking the buffer solution in the two injectors, and fixing the two injectors on the same injection pump; introducing the solution in the four injectors into a multi-channel vortex mixer through the injection pump, and receiving a product at an outlet of the multi-channel vortex mixer; and dialyzing and purifying the obtained product in ultrapure water by using a dialysis bag to obtain the spherical nanoparticles which take the vegetable protein as the carrier to wrap the natural hydrophobic drug. The invention also discloses the drug-loaded vegetable protein spherical nanoparticles obtained by the method. According to the invention, continuous reaction and preparation of the spherical nanoparticles can be realized, and the problems caused by intermittent preparation and amplification effect can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of functional nanometer materials, and in particular relates to a preparation method of drug-loaded plant protein nanoparticles. Background technique [0002] Natural plant protein has many advantages as a drug-loaded nanoparticle, such as a wide range of sources, excellent biocompatibility and biodegradability, etc. Zein and soybean protein isolate are typical representatives. The chemical structures of these two plant proteins contain both hydrophilic groups and hydrophobic groups and are amphiphilic, so that they can self-assemble to form nanostructures and be used as nanocarriers. [0003] At present, the relatively mature methods for preparing plant protein nanoparticles are anti-solvent precipitation method and pH-driven method, both of which are based on thermodynamic equilibrium self-assembly to form nanoparticles. The anti-solvent precipitation method is to dissolve the vegetable protein in a mixed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K9/50A61K31/12A61K31/047A61K31/05
CPCA61K9/5052A61K31/12A61K31/047A61K31/05
Inventor 王俊有王铭纬赵洪阳王新明邢梦媛马丁·A·科恩·斯图尔特
Owner EAST CHINA UNIV OF SCI & TECH
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