Method for changing morphological characteristics of stems, leaves and flowers of plant, and application

A technology of plant stems, leaves and shapes, applied in the field of plant cultivation, can solve problems such as gene scarcity and achieve significant economic value

Pending Publication Date: 2022-01-11
YANGTZE NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method and application for changing the morphological characteristics of plant stems, leaves and flowers, so as to solve the existing problem: when cultivating new varieties of ornamental plants and crops, genes that can cause better traits are relatively scarce

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  • Method for changing morphological characteristics of stems, leaves and flowers of plant, and application
  • Method for changing morphological characteristics of stems, leaves and flowers of plant, and application
  • Method for changing morphological characteristics of stems, leaves and flowers of plant, and application

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Embodiment Construction

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0028] The invention relates to a method for changing the morphological characteristics of plant stems, leaves and flowers.

[0029] The invention adopts the cutting-edge bioengineering technology in the biological field to introduce the BjuB033714 gene in the economic crop stem mustard into other plants in the form of transgene, so that the plants show that adventitious buds are produced on the cotyledons and can develop into complete plants. Morphological changes, early termination of inflorescence development, extra flower tissue on t...

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Abstract

The invention discloses a method for changing the morphological characteristics of the stems, the leaves and the flowers of a plant, and application, and relates to the technical field of plant cultivation. The method disclosed by the invention comprises the following steps of cloning a gene BjuB033714 in Brassica juncea var. tumidaTsen etLee, and constructing an overexpression vector which takes CAMV35S as a promoter by taking pTF101 as a target vector and taking BamH I and Sma I as restriction enzyme cutting sites; taking the whole genome cDNA of the Brassica juncea var. tumidaTsen etLee as a template, and amplifying the CDS sequence of BjuB033714 by adopting a PCR method; carrying out electrophoresis on the amplified BjuB033714 gene, and amplifying a product fragment by utilizing the recovered BjuB033714 gene; carrying out multiplication culture on a single colony which is positive in PCR amplification by using an LB liquid culture medium of 100mu g / mL spectinomycin, and extracting plasmids; and performing double enzyme digestion on the extracted plasmids by using restriction enzymes Sma I and BamH I to identify whether the vector is successfully constructed or not. The invention discloses a method capable of changing the morphological characteristics of the stems, the flowers and the like of the plant, can be used for cultivating high-quality ornamental plant varieties or new crop varieties, including ornamental flower varieties and commercial crops, and has a remarkable economic value.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method and application for changing the morphological characteristics of plant stems, leaves and flowers. Background technique [0002] Leaf shape and flower shape are important aspects of ornamental plants. High-quality ornamental plants often have the characteristics of luxuriant branches and leaves and distinctive petal shapes. In order to cultivate more ornamental plants, the inflorescence shape and flower shape of plants are often targeted. Features are improved. [0003] At present, when breeding new varieties of ornamental plants and crops, genes that can cause better traits are relatively scarce. In response to this problem, we have screened out new genes that play a role in the regulation of plant leaf and flower development. Combining molecular biology and other related methods, we can provide resources for cultivating excellent ornamental and c...

Claims

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Application Information

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IPC IPC(8): C07K14/415A01H5/00A01H5/12A01H6/20
CPCC07K14/415C12N15/8225C12N15/825C12N15/8205
Inventor 蔡兆明程春红苏少东向美琴石家宇许芳周美谷郭海燕刘金枝黄星诚
Owner YANGTZE NORMAL UNIVERSITY
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