Heavy metal Cu<2+> complete antigen and preparation method thereof

A technology of complete antigens and heavy metals, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., to achieve the effects of broad social benefits, rapid detection tools, and good economic value.
CN101775074AInactive Publication Date: 2010-07-14JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2010-07-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a heavy metal Cu<2+> complete antigen and a preparation method thereof. The preparation method comprises the following steps: Cu<2+> is reacted with a bifunctional chelating agent to form a hapten; and a Cu<2+>-chelating agent hapten is reacted with a carrier protein to form a Cu<2+>-chelating agent-carrier protein complete antigen. The antigen has better immunogenicity; and an immune mice with the antigen can be used for preparing a Cu<2+> specific monoclonal antibody and further preparing a rapid Cu<2+> detection ELISA (enzyme-linked immuno sorbent assay) kit of various heavy metals and colloidal gold immunochromatography assay test paper.
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Description

Technical field:

[0001] The invention discloses a heavy metal Cu 2+ The complete antigen and its preparation method belong to the technical field of immunological method detection. Background technique:

[0002] At present, more than 20 kinds of heavy metals are known to be highly toxic, among which lead (Pb), cadmium (Cd), mercury (Hg), copper (Cu), chromium, cobalt, nickel, tin, vanadium, etc. are used in agricultural and livestock products There are varying degrees of residues in the chemical, which pose a serious threat to human health. Therefore, all countries in the world attach great importance to the control and monitoring of heavy metal pollution in the environment and agricultural products. Traditional heavy metal detection methods such as: atomic absorption spectrometry, inductively coupled plasma emission spectrometry, etc. require large and expensive analytical instruments, which cannot be used for on-site detection, and are difficult to adapt to the environme...

Claims

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