Method for detecting JC polyoma virus through real-time fluorescent quantitative PCR (Polymerase Chain Reaction)

A real-time fluorescence quantitative, polyoma virus technology, applied in the field of biomedicine, can solve the problem of low coverage of JC virus variant strains, and achieve the effects of reducing labor and material costs, high sensitivity, and ensuring safety.
CN113913554APending Publication Date: 2022-01-11SUZHOU YAOMING KANGDE INSPECTION TESTING
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU YAOMING KANGDE INSPECTION TESTING
Publication Date
2022-01-11

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Abstract

The invention discloses a method for detecting JC polyoma virus through real-time fluorescent quantitative PCR (Polymerase Chain Reaction), provides a group of forward primers, reverse primers and probes for detecting JC virus in a biological product through real-time fluorescent quantitative PCR, and provides a PCR program for carrying out fluorescent quantitative PCR detection by utilizing the forward and reverse primers and the probes. The detection method provided by the invention can be used for detecting all JC viruses of different genotypes, is good in specificity, high in sensitivity and high in variant coverage, and compared with common virus cell culture detection, the detection time is greatly shortened, the labor and material cost is saved, and the detection method is suitable for complex detection of large-batch production of biological products.
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Description

Technical field

[0001] The present invention relates to the field of biopharmaceutical technology, and more particularly to a method of real-time fluorescence quantitative PCR to detect JC polymented viruses. Background technique

[0002] JC virus (JCV) is a human polymented virus, which is a small double DNA virus that can be divided into more than 30 genotypes. JC virus infections are worldwide, statistics in different regions around the world, and adult JCV infection rates are 50% a 90%. JC viruses may have no obvious clinical manifestations through respiratory tract infections. After the primary infection, the viral blood will be caused by the virus, the virus reaches the kidneys, when the body is immunity, such as kidney transplantation or bone marrow transplantation, pregnant women, elderly, cancer, and AIDS patients are immune function, The virus can be reactivated and replicated. At this time, the virus is likely to selectively damage the central nervous system oligogenes...

Claims

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