Method for detecting JC polyoma virus through real-time fluorescent quantitative PCR (Polymerase Chain Reaction)

Abstract

The invention discloses a method for detecting JC polyoma virus through real-time fluorescent quantitative PCR (Polymerase Chain Reaction), provides a group of forward primers, reverse primers and probes for detecting JC virus in a biological product through real-time fluorescent quantitative PCR, and provides a PCR program for carrying out fluorescent quantitative PCR detection by utilizing the forward and reverse primers and the probes. The detection method provided by the invention can be used for detecting all JC viruses of different genotypes, is good in specificity, high in sensitivity and high in variant coverage, and compared with common virus cell culture detection, the detection time is greatly shortened, the labor and material cost is saved, and the detection method is suitable for complex detection of large-batch production of biological products.

Description

Technical field [0001] The present invention relates to the field of biopharmaceutical technology, and more particularly to a method of real-time fluorescence quantitative PCR to detect JC polymented viruses. Background technique [0002] JC virus (JCV) is a human polymented virus, which is a small double DNA virus that can be divided into more than 30 genotypes. JC virus infections are worldwide, statistics in different regions around the world, and adult JCV infection rates are 50% a 90%. JC viruses may have no obvious clinical manifestations through respiratory tract infections. After the primary infection, the viral blood will be caused by the virus, the virus reaches the kidneys, when the body is immunity, such as kidney transplantation or bone marrow transplantation, pregnant women, elderly, cancer, and AIDS patients are immune function, The virus can be reactivated and replicated. At this time, the virus is likely to selectively damage the central nervous system oligogenes...

AI Technical Summary

Problems solved by technology

[0004] At present, the existing methods for detecting JC virus have a low coverage rate of JC virus mutant strains, and when the PCR method is used for detection, the detection sample DNA also contains a large amount of cellular DNA and a small amount of viral DNA, and the PCR reaction is often subject to a large number of tests. Inhibition of cellular DNA, therefore, requires the development of a highly sensitive assay that covers as many JC virus variants as possible without being affected by the large amount of background cellular DNA

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