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Strain capable of decomposing kelp

A technology of strains and kelp, applied in the direction of bacteria, biological organic part treatment, microorganism-based methods, etc., can solve the problems of destroying seaweed cell walls and high cost investment.

Active Publication Date: 2022-01-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the biological extraction process of seaweed fertilizer requires a combination of various enzymes and microbial fermentation to destroy the cell wall of seaweed, and the cost is large.

Method used

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  • Strain capable of decomposing kelp
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  • Strain capable of decomposing kelp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Collection of Carrageenan Pseudoalteromonas carrageenovora:

[0020] Cut 20 grams of fresh kelp samples into small pieces with sterile scissors, add them to an Erlenmeyer flask filled with 30 mL of sterilized physiological saline (0.9%, NaCl), and shake at 25°C and 150 r / min for 3 Hours. Draw 1 ml of the solution from the Erlenmeyer flask, add it to a 9 ml sterile saline test tube, perform 10-fold serial dilutions, and spread it on 2216E solid medium (peptone 5g / L, yeast powder 1g / L, agar Powder 20g / L, prepared by filtering seawater, pH 7.6-7.8.), cultured at 25°C for 7 days, picked different single colonies into 2216E solid medium according to the characteristics of colony shape, color, etc., streaked and purified until a single pure colony. Inoculate all the isolated strains on the alginate lyase screening medium (sodium alginate 20g / L, peptone 5g / L, yeast powder 1g / L, agar powder 20g / L, prepared by filtering seawater, pH 7.0.) plate Cultured for 3 days. Add 10% c...

Embodiment 2

[0022] Identification of Carrageenan Pseudoalteromonas carrageenovora:

[0023] The DNA of strain A1 was extracted using a bacterial genomic DNA extraction kit (Takara), and the 16S rRNA gene was amplified by PCR using 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1541R (5'-AAGGAGGTGATCCAGCCGCA-3') as primers. The PCR reaction system is 40 μL, which contains 1 μL 27F, 1 μL 1541R, 2×Mix 20 μL, ddH 2 O16 μL. The reaction conditions were 5 minutes of pre-denaturation at 94°C, followed by 30 cycles of 1 minute at 94°C, 1 minute at 55°C, 1.5 minutes at 72°C, extension at 72°C for 1.5 minutes, and cooling to 8°C. The PCR product was sent to Beijing Qingke Biological Co., Ltd. for 16S rRNA gene sequencing. After comparing the sequence with the NCBI database, the strain A1 belonged to the genus Pseudoalteromonas, which had the highest similarity (99.9%) with the published species Pseudoalteromonas carrageenovora, so the The strain is Pseudoalteromonas carrageenovora, named Pseudoalteromonas ...

Embodiment 3

[0026] The application of strain decomposing kelp, the specific steps are as follows:

[0027] Medium preparation:

[0028] 2216E liquid medium (5g / L peptone, 1g / L yeast powder, prepared by filtering seawater, pH 7.6-7.8.)

[0029] Preparation of seed solution: inoculate the A1 strain from a slant, put it into a 20 ml Erlenmeyer flask (100 ml) filled with 2216E liquid medium, and cultivate it for 2 days at 25° C. and 150 r / min.

[0030] Fermentation culture:

[0031] Dried kelp is cut into small pieces with a diameter of 2 to 3 cm, added to 2216E liquid medium, and sterilized. Insert the seed liquid, ferment and cultivate for 7-10 days.

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Abstract

The invention discloses a strain capable of decomposing kelp. The strain is from a kelp culture area of Rongcheng Ailianwan in Shandong province, is separated from the surface of the kelp, is classified and named as Pseudoalteromonas A1 of edible pelvetia silquosa, and has the functions of decomposing the kelp, destroying the cell wall of the kelp and releasing carbohydrate compounds in the kelp. The strain is preserved in the China General Microbiological Culture Collection Center on March 30, 2021, and the preservation number is CGMCC 22090. The strain provided by the invention can destroy cell walls of the kelp when being cultured at room temperature, decompose the kelp to enable the residue rate to be less than 40%, and release carbohydrates in the cell walls, thereby providing help for comprehensive utilization of the kelp.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a bacterial strain capable of decomposing kelp, and belongs to the technical field of marine biology. Background technique [0002] Laminaria belongs to the Phaeophyta, and its cell wall is rich in polysaccharides. The cell wall of kelp is mainly formed by the cross-linking of kelp starch, fucoidan sulfate and alginic acid. Proteins, glycoproteins, phenolic compounds, iodine, calcium and other halides and ions are distributed in the cell wall. [0003] Kelp is one of the main economic brown algae in my country. It not only has high edible value, but also has important commercial value in the cell wall components of kelp. [0004] Seaweed polysaccharides in kelp, including kelp starch, fucoidan sulfate and alginic acid, contain a large amount of organic compounds, calcium, magnesium, iodine and other mineral elements, which are unique plant growth regulators. These substances can Effectivel...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/20C12R1/01
CPCC05F17/20Y02W30/40
Inventor 张德超耿丽华张全斌孙忠民王晶岳洋吴宁
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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