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Universal serum-free medium supporting multiple vaccine cell adherent or suspension culture and preparation method thereof

A serum-free medium, suspension culture technology, applied in cell culture active agents, biochemical equipment and methods, animal cells, etc., can solve the lack of versatility and stability, unfavorable development and large-scale production of veterinary biological products, etc. problems, to achieve the effect of being conducive to large-scale production, shortening the time of domestication, simple and efficient

Pending Publication Date: 2022-01-18
无锡多宁生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most vaccine culture media tend to be customized and individualized, lacking versatility and stability
Moreover, in custom-made personalized development, relatively expensive animal-derived proteins such as transferrin are often added to the serum-free medium, which is not conducive to the development and large-scale production of veterinary biological products

Method used

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  • Universal serum-free medium supporting multiple vaccine cell adherent or suspension culture and preparation method thereof
  • Universal serum-free medium supporting multiple vaccine cell adherent or suspension culture and preparation method thereof
  • Universal serum-free medium supporting multiple vaccine cell adherent or suspension culture and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] This example is a universal serum-free medium suitable for vaccine cells from a variety of different sources; the materials used were purchased from Sigma Company unless otherwise specified; the BHK21 cell line used was Tianxinhe’s suspension BHK21 cells, and the MDCK adherent cells used and PK-15 adherent cells were donated to Guangzhou Qizhi Biological Co., Ltd. Prepare the general-purpose serum-free culture medium of this research embodiment 1 according to each component interval described in claim 2, and its specific components are as follows:

[0070] amino acid group

[0071]

[0072] vitamin group

[0073] ingredients Contentmg / L ingredients Contentmg / L alpha-lipoic acid 1.2 Nicotinamide 3.5 Cyanocobalamin 1.23 Thiamine hydrochloride 3.3 biotin 3.06 riboflavin 0.33 folic acid 4.34 p-aminobenzoic acid 1.5 Vitamin C 21 Pyridoxine 4.4 thbrthdrexvbdr 3.4 Choline chloride 86 Inositol 98 ...

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Abstract

The invention discloses a universal serum-free medium supporting multiple vaccine cell adherent or suspension culture and a preparation method thereof. The universal serum-free medium is prepared from the following raw materials: an amino acid group, a vitamin group, an inorganic salt and trace element group, and a lipid, hydrolysate and additive group. The medium does not contain animal serum, is low in cost, supports BHK21 high-density full suspension culture and MDCK and PK-15 cell serum-free adherent culture, and is clear in components, easy to prepare and convenient to use. The medium can be simultaneously suitable for serum-free adherent growth and passage of BHK21 suspension cells, MDCK and PK-15 cells, has a culture effect which is not inferior to that of a medium which is individually developed for different cells in the market, and is extremely high in universality. The preparation method is simple, quick, high in efficiency and favorable for large-scale production.

Description

technical field [0001] The invention relates to the field of serum-free animal cell culture biotechnology, in particular to a universal serum-free medium supporting a variety of vaccine cell attachment or suspension culture and a preparation method thereof. Background technique [0002] BHK-21 (Baby Hamster Kidney Cell) is baby hamster kidney cells, which are derived from the kidneys of five 1-day-old hamsters of unidentified sex. The original cell line is an adherent fibroblast, which can grow in suspension and be used for the production of viral vaccines after numerous subcultures. Common vaccines for foot-and-mouth disease and rabies, as well as Japanese encephalitis virus, Zika virus, and certain strains of influenza virus can also be propagated in the cells. Therefore, the cell occupies a major position in veterinary vaccines, with a huge market share and broad prospects. [0003] MDCK (Madin-Darby canine kidney cell) is canine kidney epithelial cells, which were isol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/02
CPCC12N5/0686C12N2500/90C12N2500/32C12N2501/999C12N2500/38C12N2500/12C12N2500/14C12N2500/20C12N2500/16C12N2500/22C12N2500/24C12N2500/30C12N2500/36C12N2500/34C12N2500/76
Inventor 王龙姚芸王猛
Owner 无锡多宁生物科技有限公司
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