Cis-platinum and Tri-1 combined anti-lung-cancer pharmaceutical composition and application thereof
A technology of anti-lung cancer drugs and compositions, which is applied in the direction of drug combinations, anti-tumor drugs, and active ingredients of heterocyclic compounds, etc., can solve the problems of easy metastasis of lung cancer, missed treatment period, and reduced anti-cancer effect, achieving low toxicity, Reduce the dosage and enhance the inhibitory effect
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Embodiment 1
[0027] This embodiment provides a combined anti-lung cancer method of cisplatin and Tri-1. This embodiment detects that when the two are used in combination, Tri-1 reverses the drug resistance of tumor cells to cisplatin. The specific steps are as follows:
[0028] (1) The effect of cisplatin monotherapy on the proliferation of normal lung cancer cell A549 and cisplatin-resistant lung cancer cell A549 / DDP was detected by MTT: both cells were treated with 10% fetal bovine serum, 100 kU / L penicillin, 100 mg / L streptomycin in DMEM high-glucose medium, 37°C, 5% CO by volume 2 cultivated under conditions. The cells were trypsinized and counted with a hemocytometer. Inoculate in a 96-well cell culture plate at a volume of 100 μL per well (cell inoculation volume is 8000-10000 per well, selected according to cell size and growth rate), and the volume ratio is 5% CO 2 After 24 hours of cultivation in a 37°C incubator, cisplatin with different concentration gradients was added, and t...
Embodiment 2
[0031] This embodiment provides a method for combining cisplatin and Tri-1 against lung cancer. When the two are used in combination, Tri-1 enhances the inhibitory effect of cisplatin on cell migration. The specific steps are as follows:
[0032] Scratch test to detect the effect on cell migration: First, use a Marker pen on the back of the 6-well plate, compare it with a ruler, and draw horizontal lines evenly, approximately every 1.0 cm, across the wells, 3 lines per well. After the cells to be used were digested with trypsin, they were counted with a hemocytometer. The cell density is 5×10 5 Cells / well were inoculated in a 6-well cell culture plate. 24 hours after inoculating the cells, use a 200uL pipette tip to compare the ruler, and scratch perpendicular to the horizontal line on the back as much as possible. Then wash the cells twice with PBS buffer, remove the scratched cells, take pictures under the microscope, record the scratches at 0 hours after adding the drug, t...
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