Preparation method and application of traditional Chinese medicine composition for treating bile reflux gastritis
A composition and drug technology, applied in the field of medicine, can solve the problems of low transfer rate and low purity of evodia rutaecarpa alkaloids, achieve the effects of preventing and treating gastric mucosal damage, improving clinical effects, and reducing toxic and side effects
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Embodiment 1
[0055] Example 1 Preparation of Coptis Rhizoma Total Alkaloids
[0056] 1.1 Sample preparation
[0057] Weigh 1200g of Coptis decoction pieces (Bozhou Shuzhong Pharmaceutical Co., Ltd., batch number 200528), crush them through an 8mm sieve, add 10 times the amount of 70% ethanol, and reflux extraction twice, each time for 1 hour, and filter the extract with a 200-mesh filter cloth. Over, combine filtrate (retain sample), reclaim ethanol and be concentrated to every milliliter of 0.5g crude drug, get supernatant, pass through pretreated D101 type macroporous adsorption resin column (600ml macroporous adsorption resin, inner diameter 3.5cm glass column) and adsorbed overnight. 2BV was eluted with water, and then 40% ethanol was eluted at a flow rate of 2BV / hour for 6 column volumes. The eluents were combined, concentrated under reduced pressure, and dried. Determine the content of total alkaloids and berberine hydrochloride, coptisine, epiberberine, palmatine, jatrorrhizine, a...
Embodiment 2
[0077] Example 2 Preparation of Total Alkaloids from Evodia Evodia
[0078] 2.1 Sample preparation
[0079] Weigh 200g decoction pieces of Evodia rutaecarpa (Hebei Xinjingyuan Pharmaceutical Co., Ltd., batch number 1812002). Add 10 times of 70% ethanol for extraction, reflux extraction twice, each time for 1 hour, filter the extract with a 200-mesh filter cloth, combine the filtrate (retained sample), recover the ethanol and concentrate to about 0.5g crude drug / ml, and filter to obtain the supernatant Add 80ml of 95% ethanol to the insoluble matter and ultrasonically dissolve it at 60°C, then add it to the supernatant, mix and heat until it is nearly dissolved. At this time, the alcohol concentration of the sample solution is about 40%. 400ml D101 macroporous resin (resin initial alcohol concentration is 30%) was adsorbed overnight, and 10BV was eluted with 60% ethanol and 10BV were eluted with 95% ethanol respectively. -Combined 95% of the eluents from the eluents were conc...
Embodiment 3
[0099] Example 3 Preparation of Coptis Rhizoma and Evodia Evodia Sample Composition Sample
[0100] Composition 1:
[0101] The samples of Coptidis Rhizoma and Evodia rutaecarpa obtained in Example 1 and Example 2 were respectively mixed according to the ratio of 10:1 of crude drug, dissolved in 95% ethanol, concentrated and dried under reduced pressure to form dry powder.
[0102] Composition 2:
[0103] The Coptidis rhizome and Evodia rutaecarpa samples obtained in Example 1 and Example 2 were taken respectively, mixed according to the ratio of 6:1 of raw medicinal materials, dissolved in 95% ethanol, concentrated and dried under reduced pressure to form dry powder.
[0104] Composition 3:
[0105] The samples of Coptidis Rhizoma and Evodia rutaecarpa obtained in Example 1 and Example 2 were respectively mixed according to the ratio of 8:1 of crude drug, dissolved in 95% ethanol, concentrated and dried under reduced pressure to form a dry powder.
[0106] Composition 4: ...
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