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Pepper straw degradation-in-situ returning change

A chili straw, in-situ technology, applied in the field of microorganisms, can solve the problems such as the degradation efficiency needs to be improved, and achieve the effect of easy large-scale production, low cost and simple operation method

Pending Publication Date: 2022-01-28
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the inventor found that the degradation efficiency of the existing bacterial agents used for the degradation of pepper stalks still needs to be improved.

Method used

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  • Pepper straw degradation-in-situ returning change
  • Pepper straw degradation-in-situ returning change
  • Pepper straw degradation-in-situ returning change

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1 raw material

[0048] (1) Collection of samples containing straw-degrading microorganisms

[0049] 200g of soil for long-term (more than two years) pepper straw returning to the field.

[0050] (2) Medium carbon source

[0051] After the pepper stalks are air-dried, they are soaked in 1% NaOH solution for 24 hours to remove lignin, rinsed with water several times until the pH of the rinse solution is neutral (7.0), dried at 80°C, and cut into 2 cm long pieces for later use.

[0052] (3) culture medium

[0053] 5.5g peptone, 1.6g yeast extract, 2.1g CaCO 3 , 4.5g NaCl, 0.4g MgSO 4 ·7H 2 O, 0.8g K 2 HPO 4, 1% (w / v, 10g added to 1000ml) pepper straw (2cm fragment), 1,000ml deionized water, pH=7.2. After the medium is prepared, it must be sterilized at 121°C for 20 minutes.

[0054] 2 Screening of complex microbial strains for synergistic degradation of pepper straw

[0055] (1) Put 20g of long-term pepper straw returning soil into a 500-ml Erlenmeyer flask, add...

Embodiment 2

[0060] Test the degradability of the bacterial agent of Example 1 on pepper stalks. The specific method of the degradation performance test is: crush the pepper stalks, mix them uniformly with the bacterial agent at a mass ratio of 1:100, place them in a mesh bag, and then , bury the mesh bag in the ground, take it out regularly, test the quality of pepper straw, and calculate the degradation rate. The specific test results are shown in Table 1, Table 2, figure 1 , figure 2 shown.

[0061] Table 1 is the degradation rate table of pepper straw

[0062]

[0063]

[0064] Table 2 Actual mass of pepper straw in field soil after in situ return to field after inoculum treatment

[0065]

[0066] It can be seen that the method of the present invention can quickly screen, enrich and cultivate the bacterial flora with the ability to degrade pepper stalks, so that it has a quantitative advantage in the total bacteria, and the obtained bacterial liquid is used for returning t...

Embodiment 3

[0068] In order to better explore the degradation law and later commercial application of pepper stalks, the application also sequenced the strains in the bacterial agent obtained in Example 1 (the test method is a commercial high-throughput sequencing technology); the flora sequence as follows:

[0069] OTU11

[0070] Chryseobacterium taichungense strain CC-TWGS1-8 16S ribosomal RNA,partial sequence

[0071] ORGANISM Chryseobacterium taichungense

[0072] Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;

[0073] Weeksellaceae; Chryseobacterium group; Chryseobacterium.

[0074] ORIGIN SEQ ID NO:1

[0075] OTU208

[0076] Stenotrophomonas acidaminiphila strain AMX 19 16S ribosomal RNA,partial sequence

[0077] ORGANISM Stenotrophomonasacidaminiphila

[0078] Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;

[0079] Xanthomonadaceae; Stenotrophomonas.

[0080] ORIGIN SEQ ID NO:2

[0081] OTU225

[0082] Lentimicrobium saccharophilum strain TBC...

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PUM

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Abstract

The invention belongs to the field of microorganisms, and discloses a pepper straw degradation-in-situ returning change method. The method comprises the following steps: drying pepper straws in air, removing lignin, adjusting the pH value to be neutral, drying, and slicing for later use; preparing a culture medium containing the pepper straws by taking the sliced pepper straws as a carbon source, and sterilizing; dividing the soil subjected to long-term pepper straw returning into a plurality of groups of samples, and respectively carrying out primary standing culture in the culture medium under a sealed condition; then uniformly shaking, taking a mixed solution, putting the mixed solution into the culture medium containing pepper straws, and carrying out shake culture; screening a plurality of groups of samples, retaining the samples with high decomposition capability, performing secondary static culture in the culture medium containing the pepper straws again, and repeating the screening and secondary static culture process for multiple times until the degradation capability and the microbiome are kept stable. The actual mass of the pepper straws returned to the field in situ after being treated by the screened microbial agent is obviously reduced in field soil.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to the degradation of capsicum stalks and the change in situ returning to the field. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] At present, using the wide adaptability and multifunctionality of microorganisms to transform straw has been increasingly valued by scientific researchers at home and abroad, because microorganisms have many advantages in straw transformation, such as multi-purpose, high nutritional value, short cycle, and regeneration. As a new way to utilize straw resources, it has broad prospects and will play an increasingly important role in agricultural pro...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12N1/20C05F11/00C05F11/08C05F17/20C12R1/01
CPCC12N1/02C12N1/20C05F11/00C05F11/08C05F17/20Y02W30/40
Inventor 陈之群闫丽孙爱德彭金鑫胡志杰杨华化柏全
Owner LINYI UNIVERSITY