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Preparation method of novel bi-crosslinked antibacterial hydrogel for repairing wound surfaces of burns and scalds

A technology for wound repair and hydrogel, which is applied in the field of preparation of new double cross-linked antibacterial hydrogel, can solve the problems of unfavorable wound tissue repair, increase wound tissue cell burden, and high wound toxicity, and achieve good pH responsiveness, Ideal performance and reproducible results

Pending Publication Date: 2022-02-01
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the current medical dressings for burns and scalds, they basically focus on the repair of skin tissue. For the antibacterial treatment of burns and scald wounds, additional medication is used. Cell burden, not conducive to wound tissue repair

Method used

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  • Preparation method of novel bi-crosslinked antibacterial hydrogel for repairing wound surfaces of burns and scalds
  • Preparation method of novel bi-crosslinked antibacterial hydrogel for repairing wound surfaces of burns and scalds
  • Preparation method of novel bi-crosslinked antibacterial hydrogel for repairing wound surfaces of burns and scalds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1, preparation of ZIF-8 nanoparticles loaded with polyhexamethylene biguanide

[0028] Dissolve 0.02g of polyhexamethylene biguanide and 0.66g of 2-methylimidazole in 50ml of methanol, then add 0.30g of zinc nitrate hexahydrate to it, stir at room temperature for 24 h, centrifuge to collect the precipitate, and wash the precipitate with ethanol After washing for 3 times, they were dried in a vacuum oven for 12 h to obtain ZIF-8 nanoparticles loaded with polyhexamethylene biguanide. figure 1 It is a SEM image of ZIF-8 nanoparticles, which shows that the prepared sample is a rhombic dodecahedron with a diameter of 400 nm. figure 2 It is the XRD detection result graph of ZIF-8 nanoparticles, which shows that the crystal structure of ZIF-8 nanoparticles is not changed whether the polyhexamethylene biguanide is loaded or not. image 3 The UV-Vis map shows that polyhexamethylene biguanide is loaded into ZIF-8 nanoparticles.

[0029] Step 2. Preparation of modified hum...

Embodiment 2

[0034] Step 1, preparation of ZIF-8 nanoparticles loaded with polyhexamethylene biguanide:

[0035] Dissolve 0.1 g of polyhexamethylene biguanide and 3 g of 2-methylimidazole in 500 ml of methanol, then add 1.5 g of zinc nitrate hexahydrate to it, stir at room temperature for 12 h, collect the precipitate by centrifugation, and wash the precipitate with ethanol After 3 times, dry in a vacuum oven for 12 h to obtain ZIF-8 nanoparticles loaded with polyhexamethylene biguanide;

[0036] Step 2. Preparation of modified human-like collagen (HLC):

[0037]Under magnetic stirring, 5 g of human-like collagen was dissolved in 200 ml of neutral PBS buffer, and 3 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride was sequentially added thereto Salt (EDC) and 3g of N-hydroxysuccinimide (NHS), and then add 5g of 3-aminophenylboronic acid hemisulfate (APBA), and adjust the pH of the solution to 4.5 and stir for 12 h, The reaction mixture was put into a dialysis bag (the mole...

Embodiment 3

[0041] Step 1, preparation of ZIF-8 nanoparticles loaded with polyhexamethylene biguanide:

[0042] Dissolve 0.2 g of polyhexamethylene biguanide and 7 g of 2-methylimidazole in 500 ml of methanol, then add 3 g of zinc nitrate hexahydrate, stir at room temperature for 24 h, collect the precipitate by centrifugation, and wash the precipitate with ethanol for 3 After drying for 12 h in a vacuum drying oven, ZIF-8 nanoparticles loaded with polyhexamethylene biguanide were obtained;

[0043] Step 2. Preparation of modified human-like collagen (HLC):

[0044] Under magnetic stirring, 5 g of human-like collagen was dissolved in 200 ml of neutral PBS buffer, and 3 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride was sequentially added thereto Salt (EDC) and 3g of N-hydroxysuccinimide (NHS), then add 5g of 3-aminophenylboronic acid hemisulfate (APBA), and adjust the pH of the solution to 5 and stir for 6h, then put The reaction mixture was put into a dialysis bag (th...

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Abstract

The invention discloses a preparation method of novel bi-crosslinked antibacterial hydrogel for repairing wound surfaces of burns and scalds, which specifically comprises the following steps: firstly, preparing polyhexamethylene biguanide-loaded ZIF-8 nanoparticles, grafting 3-aminophenylboronic acid to human-like collagen to obtain modified human-like collagen, activating sodium alginate by using EDC and NHS, and finally, performing composite crosslinking on sodium alginate, the ZIF-8 nanoparticles and the modified human-like collagen to form double-network hydrogel with a stable space structure, and performing Co60 irradiation sterilization to obtain the novel double-crosslinking antibacterial hydrogel for repairing wound surfaces of burns and scalds. The preparation method is simple and easy to implement and good in reproducibility, wherein the prepared bi-crosslinking antibacterial hydrogel has long-acting slow-release antibacterial capacity and good epithelial cell growth promoting capacity, has ideal performance in the aspect of burn and scald wound repair and is wide in application prospect.

Description

technical field [0001] The invention relates to a preparation method of a novel double-crosslinked antibacterial hydrogel for repairing burn and scald wounds, and belongs to the technical field of biomedical materials. Background technique [0002] Burns are one of the most devastating skin traumas, causing more than 180,000 deaths each year. Infection is also one of the main causes of burn morbidity and mortality. Pathogens can settle on the wound surface within the first 48 hours, and the nosocomial wound infection rate is about 31%. Burns and scalds are injuries mainly characterized by damage to skin structure and function. Compared with other wounds, burns with larger wound area, more irregularity and severe bleeding are more likely to be infected and heal slowly. The core content of its treatment is to effectively control wound infection and provide a beneficial microenvironment for healing, so as to accelerate the process of cell repair and regeneration. Therefore, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08L89/00C08L79/00C08L87/00C08L5/04A61L27/18A61L27/20A61L27/22A61L27/50A61L27/52A61L27/54A61L27/60
CPCC08J3/075C08J3/246A61L27/60A61L27/54A61L27/52A61L27/50A61L27/227A61L27/20A61L27/18C08J2389/00C08J2479/00C08J2487/00C08J2405/04A61L2400/12A61L2300/404A61L2300/602A61L2300/41A61L2300/216C08L89/00C08L5/04C08L79/00
Inventor 惠俊峰李娜樊妙琳王明明范代娣郑晓燕
Owner NORTHWEST UNIV
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