Hepatic failure detection reagent and application thereof in hepatic failure detection

Abstract

The invention provides a hepatic failure detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A, which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10 mM; a reagent B, which is prepared by mixing 0.01-10 U / 10 [mu] L of glycosamine acylase and 0.01-10 U / 10 [mu] L of sialidase, wherein the pH value of the mixed solution is 4-9; a reagent C, which is prepared by dissolving 8-aminopyrene-1, 3, 6-trisulfonic acid in DMSO (dimethylsulfoxide), and the concentration of the reagent C is 0.01 mM to 1M; and a reagent D: a stop solution. According to the invention, a glycome map in serum is determined by a detection reagent, and a peak value is quantified for statistical analysis, so that the establishment method of the hepatic failure serum glycome map model is provided for detecting hepatic failure.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a detection method for liver failure, in particular to a detection method for liver failure based on the specific fingerprint of serum glycoprotein oligosaccharide chain detection (G-Test). Background technique [0002] Liver failure (Liver Failure) refers to severe liver damage caused by a variety of factors, leading to serious impairment or decompensation of its synthesis, detoxification, excretion and biotransformation functions, resulting in coagulation mechanism disorders, jaundice, hepatic encephalopathy , ascites, etc. as the main manifestations of a group of clinical syndrome. Generally can be divided into acute liver failure (Acute Liver Failure, ALF) and chronic liver failure (Chronic Liver Failure, CLF). The clinical manifestations are extreme fatigue, and severe gastrointestinal symptoms such as obvious anorexia, abdominal distension, nausea, and vomiting. As one o...

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Problems solved by technology

However, the mass spectrometry instrument is precise, the operation of the equipment is complicated, and the mass spectrometer is expensive, so it is not suitable for popularization and use in clinical practice; (3) capillary electrophoresis (CE): capillary electrophoresis is low in cost, high in column efficiency, high in sensitivity, fast in speed, and easy to inject. The amount is small and the operation is simple, but the repeatability is not high and the stability is not as good as HPLC

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