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Application of short-chain fatty acid in prevention or/and treatment of liver injury

A technology for short-chain fatty acids and liver damage, applied in the field of medicine, can solve the problems of high requirements for storage conditions, unclear effector molecules, and complicated extraction methods, and achieves significant prevention and treatment effects, low cost, and improvement of intestinal epithelial barrier effects.

Pending Publication Date: 2022-02-15
SHANDONG SHANWEI IMMUNOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction methods of the above components are cumbersome, the cost is high, and the components are complex, the effect molecules are not clear, and the storage conditions are high.

Method used

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  • Application of short-chain fatty acid in prevention or/and treatment of liver injury
  • Application of short-chain fatty acid in prevention or/and treatment of liver injury
  • Application of short-chain fatty acid in prevention or/and treatment of liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The influence of embodiment 1 propionic acid on alcoholic liver injury

[0039] 1. Determination of the effect of propionic acid on the survival rate of hepatocytes exposed to alcohol

[0040] It should be noted that in the present invention, the experimental cells are human hepatoma cells HepG2, and the medium used for cell culture is Hyclone containing 10% fetal bovine serum and 1% double antibody (penicillin 100U / mL, streptomycin 100 μg / mL). High glucose DMEM medium.

[0041] The specific measurement method is as follows:

[0042] After the recovery of HepG2 cells, after passage once, the cells in the logarithmic growth phase were collected and inoculated in a 96-well plate at a concentration of 5,000 cells per well, and placed at 37°C, 5% CO 2 Incubate overnight in the incubator. In the experiment, a blank group, a negative control group and an administration group were set up, with 5 duplicate wells in each group, the original medium was sucked and discarded, th...

Embodiment 2

[0090] The influence of embodiment 2 butyric acid on alcoholic liver injury

[0091] 1. Determination of the effect of butyric acid on the survival rate of hepatocytes exposed to alcohol

[0092] The specific assay method is described in Example 1.

[0093] 2. Determination of the effect of butyric acid on alcoholic liver injury model mice

[0094] 2.1 Construction of mouse alcoholic liver injury model (NIAAA method)

[0095] Forty-eight 8-week-old male C57BL / 6 (body weight > 20g) mice of SPF grade were randomly divided into 3 groups: blank control group (Control), alcohol model group (EtOH), supplemented with 100mM sodium butyrate Modules (EtOH+B), 12 in each group. The specific modeling and sampling methods are as described in Example 1.

[0096] 2.2 Determination of liver index

[0097] The specific assay method is described in Example 1.

[0098] 2.3 Determination of short-chain fatty acids in intestinal contents and liver tissue

[0099] The specific assay method i...

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Abstract

The invention discloses application of short-chain fatty acid in prevention or / and treatment of liver injury. The short-chain fatty acid is a product of intestinal flora glycolysis dietary fibers, belongs to an endogenous substance, is small in toxic and side effects, simple in structure, easy to metabolize, convenient to obtain and low in price, and can improve liver functions, inhibit liver fatty degeneration, maintain intestinal health and prevent or / and treat liver injury.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of short-chain fatty acids in preventing or / and treating liver damage. Background technique [0002] Liver injury is a common clinical disease, mainly divided into open liver injury, pathological liver injury, closed liver injury and chemical liver injury. With the development of society, the incidence of chemical liver injury is increasing day by day, among which alcoholic liver injury caused by long-term or heavy drinking is particularly high, and has become one of the common diseases that seriously endanger human health. The course of alcoholic liver injury mainly includes alcoholic fatty liver, alcoholic hepatitis and cirrhosis. In our country, the incidence of alcoholic liver injury and its related mortality are increasing year by year, so it is very important to find effective methods to prevent and treat alcoholic liver injury. At present, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/19A61P1/16
CPCA61K31/19A61P1/16A61K2300/00
Inventor 徐琦穆岩王泉博查尔斯·雷伊·麦凯
Owner SHANDONG SHANWEI IMMUNOTECH CO LTD
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