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Screening method of sulfate reducing bacteria capable of degrading polycyclic aromatic hydrocarbon

A screening method and polycyclic aromatic hydrocarbon technology, applied in the field of environmental microorganisms, can solve the problem of effective degradation of PAHs in anaerobic areas, low potential sensitivity, and restrict the further development of PAHs-contaminated soil and groundwater anaerobic bioremediation technology in anaerobic areas and application research to achieve the effect of overcoming the low enough environmental oxidation-reduction potential, simple operation, and wide application prospects

Pending Publication Date: 2022-02-18
TSINGHUA UNIV
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Problems solved by technology

[0005] SRB has been extensively screened by many researchers due to its important role. However, most of the SRB obtained by the existing screening are Desulfovibrio, which is not sensitive to oxygen and redox potential, and cannot detect PAHs in anaerobic regions. Effective degradation restricts the further development and application of PAHs-contaminated soil and groundwater anaerobic bioremediation technology in anaerobic areas

Method used

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  • Screening method of sulfate reducing bacteria capable of degrading polycyclic aromatic hydrocarbon
  • Screening method of sulfate reducing bacteria capable of degrading polycyclic aromatic hydrocarbon
  • Screening method of sulfate reducing bacteria capable of degrading polycyclic aromatic hydrocarbon

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Embodiment 1

[0054] Such as figure 1 As shown, the screening method for sulfate-reducing bacteria that can anaerobically degrade phenanthrene includes: enrichment of sulfate-reducing bacteria for anaerobic biodegradation of phenanthrene, dilution coating of strains-anaerobic glove box and stacked dish sandwich method combined screening , Separation and purification of bacterial strains, determination of the ability of degrading phenanthrene and reducing sulfate radicals obtained by screening obtained pure sulfate-reducing bacteria, and preservation of strains.

[0055] Enrichment of Sulfate-Reducing Bacteria for Anaerobic Biodegradation of Phenanthrene:

[0056] Add 50mL of basal medium into a small-mouth anaerobic bottle with a volume of 300mL, then vacuumize for 1min, expose to high-purity nitrogen for 15min, seal the anaerobic bottle with a butyl rubber stopper, and sterilize at 121°C and high pressure for 20min. Transfer the anaerobic bottle to an anaerobic glove box, remove the butyl...

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Abstract

The invention provides a screening method of sulfate reducing bacteria capable of degrading polycyclic aromatic hydrocarbon, which comprises the following steps: carrying out enrichment culture on a sample containing a sulfate reducing flora to obtain an enriched bacteria solution, and then carrying out anaerobic culture on the enriched bacteria solution by using a stack dish sandwich method; in the dish stacking and interlayer method, a used solid culture medium contains sodium sulfite and L-cysteine hydrochloride, and polycyclic aromatic hydrocarbon and the enriched bacterial liquid are coated under an anaerobic condition. According to the method, the anaerobic degradation strain with PAHs degradation capability can be efficiently obtained, and the method is simple to operate and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a screening method for sulfate-reducing bacteria capable of degrading polycyclic aromatic hydrocarbons. Background technique [0002] Polycyclic Aromatic Hydrocarbons (PAHs for short) are mainly a class of highly toxic substances produced by oil pollution, oil tanker leakage, vehicle exhaust, coal and petroleum and other natural fuels. Or the toxic organic pollutants formed by the fusion of two or more benzene rings. PAHs have a relatively stable chemical structure and strong hydrophobicity, so they are widely distributed and exist stably in the environment, and it is easy to cause a large amount of high molecular weight PAHs to accumulate. At the same time, the fat solubility of PAHs makes them easily absorbed by animals and difficult to excrete, and they can be gradually enriched in animals and plants through the food chain. Since most PAHs have "three-ca...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A62D3/02C12R1/01A62D101/20
CPCC12N1/20C12N1/02A62D3/02A62D2101/20
Inventor 王慧张作涛
Owner TSINGHUA UNIV
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