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Complex microbial inoculant for degrading pesticide residues as well as preparation method and application of complex microbial inoculant

A technology for compound bacterial agent and pesticide degradation, which is applied in the biological field to achieve the effects of convenient fermentation, energy saving and environmental protection in the production process, and broad market prospects.

Pending Publication Date: 2022-03-01
上海荣吉明微生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no effective method for degrading the residues of pesticides and fertilizers.

Method used

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  • Complex microbial inoculant for degrading pesticide residues as well as preparation method and application of complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of the composite bacterial agent for degrading pesticide residues provided by the present embodiment comprises the following steps:

[0024] Step 1. Activation of strains

[0025] Activate Rhodopseudomonas capsulata, Ectothiorhodospira sp and Rhodopseudomonas palustris respectively.

[0026] Wherein, the rhodopseudomonas capsulata (Rhodopseudomonas capsulata) activation medium is: sodium acetate 310g, NaCl 105g, (NH 4 ) 2 SO 4 0.13g, MgSO 4 0.12g, CaCl 2 50mg, yeast extract 0.11g, MgSO 4 215mg, peptone 10mg, KH 2 PO 4 0.10g, K 2 HPO 4 0.13g, FeSO 4 5mg, glutamic acid 0.12mg, distilled water 1000mL, pH 7.1. The activation temperature is 30-34°C, and the activation time is 48-72 hours.

[0027] The culture medium of Rhodopseudomonas palustris (Rhodop seudanonas palustris) is: sodium acetate is 3.8g / L, ammonium chloride 1.3g / L, yeast extract 0.7g / L, trace element 0.8mL / L, wherein, trace element is made of FeCl 3 ·6H 2 O 5mg, CuS...

Embodiment 2

[0036] The preparation method of the composite bacterial agent for degrading pesticide residues provided by the present embodiment comprises the following steps:

[0037] Step 1. Activation of strains

[0038] Activate Rhodopseudomonas capsulata, Ectothiorhodospira sp and Rhodopseudomonas palustris respectively.

[0039] Wherein, the rhodopseudomonas capsulata (Rhodopseudomonas capsulata) activation medium is: sodium acetate 310g, NaCl 105g, (NH 4 ) 2 SO 40.13g, MgSO 4 0.12g, CaCl 2 50mg, yeast extract 0.11g, MgSO 4 215mg, peptone 10mg, KH 2 PO 4 0.10g, K 2 HPO 4 0.13g, FeSO 4 5mg, glutamic acid 0.12mg, distilled water 1000mL, pH 7.1. The activation temperature is 30-34°C, and the activation time is 48-72 hours.

[0040] The culture medium of Rhodopseudomonas palustris (Rhodop seudanonas palustris) is: sodium acetate is 3.8g / L, ammonium chloride 1.3g / L, yeast extract 0.7g / L, trace element 0.8mL / L, wherein, trace element is made of FeCl 3 ·6H 2 O 5mg, CuSO ...

Embodiment 3

[0049] The preparation method of the composite bacterial agent for degrading pesticide residues provided by the present embodiment comprises the following steps:

[0050] Step 1. Activation of strains

[0051] Activate Rhodopseudomonas capsulata, Ectothiorhodospira sp and Rhodopseudomonas palustris respectively.

[0052] Wherein, the rhodopseudomonas capsulata (Rhodopseudomonas capsulata) activation medium is: sodium acetate 310g, NaCl 105g, (NH 4 ) 2 SO 4 0.13g, MgSO 4 0.12g, CaCl 2 50mg, yeast extract 0.11g, MgSO 4 215mg, peptone 10mg, KH 2 PO 4 0.10g, K 2 HPO 4 0.13g, FeSO 4 5mg, glutamic acid 0.12mg, distilled water 1000mL, pH 7.1. The activation temperature is 30-34°C, and the activation time is 48-72 hours.

[0053] The culture medium of Rhodopseudomonas palustris (Rhodop seudanonas palustris) is: sodium acetate is 3.8g / L, ammonium chloride 1.3g / L, yeast extract 0.7g / L, trace element 0.8mL / L, wherein, trace element is made of FeCl 3 ·6H 2 O 5mg, CuS...

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Abstract

The invention discloses a complex microbial inoculant for degrading pesticide residues as well as a preparation method and application of the complex microbial inoculant for degrading the pesticide residues, and the active ingredients of the complex microbial inoculant for degrading the pesticide residues are Rhodopseudomonas capsulatus, Rhodotorula sp and Rhodopseudomonas palustris. The complex microbial inoculant for degrading the pesticide residues comprises the following components: Rhodopseudomonas capsulatus, Rhodotorula sp and Rhodopseudomonas palustris. Tests prove that the complex microbial inoculant can effectively degrade pollution such as herbicides, insecticides, bactericides, hydrogen sulfide, organophosphorus, chloride ions, nitrite and polycyclic aromatic hydroxyl.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a composite microbial agent for degrading pesticide residues, a preparation method and application thereof. Background technique [0002] With the development of agriculture, a large amount of pesticides and fertilizers are used in the agricultural production process. Therefore, in the process of planting crops, there are a large number of pesticides and fertilizers residues. There are many reasons that cause and affect pesticides residues. Factors and methods of using pesticides are the main factors affecting pesticide residues. Some of the pesticides applied to crops are attached to the crops, some are scattered in the soil, air and water environment, and some of the pesticides remaining in the environment will be absorbed by plants. Pesticide residues reach humans and animals directly through plant fruits, water, and air, or pass through the environment and food chain to humans a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/01A62D101/04C02F101/30
CPCC12N1/20A62D3/02B09C1/10C02F3/34A62D2101/04C02F2101/306
Inventor 黄传志
Owner 上海荣吉明微生物工程有限公司
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