High performance liquid chromatography detection method of berberine intermediate

Abstract

The invention belongs to the field of medical intermediate detection, and particularly discloses a high performance liquid chromatography detection method of berberine intermediates, the intermediates are homopiperony lamine, the chemical name is 3, 4-methylenedioxy phenylethylamine, and the detection method comprises the steps of determination of chromatographic conditions, preparation of a test solution, preparation of a reference solution and preparation of a standard substance. Calculating a standard curve; respectively and precisely measuring the test solution and the reference solution, injecting into a liquid chromatograph, recording a chromatogram, and calculating the content by peak area according to an external standard method. The detection method disclosed by the invention is good in specificity, high in sensitivity and high in separation degree, and durability, linearity and the like can meet requirements.

Description

technical field

[0001] The invention belongs to the field of detection of pharmaceutical intermediates, and specifically discloses a high performance liquid chromatography detection method for berberine intermediates. Background technique

[0002] Berberine, also known as berberine, is an important antibiotic, which has antibacterial effects on hemolytic streptococcus, Staphylococcus aureus, gonorrhoeae, Freund's, Shigella, etc., and can enhance leukocyte phagocytosis It also has different degrees of inhibitory effects on Mycobacterium tuberculosis and Yersinia pestis, and also has inhibitory effects on amoebic bacteria in rats. Berberine hydrochloride (commonly known as berberine hydrochloride) has been widely used in the treatment of gastroenteritis, bacillary dysentery, etc., and has a certain effect on tuberculosis, scarlet fever, acute tonsillitis and respiratory tract infection. Coptis chinensis, Phellodendron phellodendri, Sangezhen and Shigonggong are commonly used ...

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