High performance liquid chromatography detection method of berberine intermediate

A high-performance liquid chromatography and detection method technology, which is applied in the field of pharmaceutical intermediate detection, can solve the problem that the rapid detection method has not been reported in relevant literature, and achieves the effects of high sensitivity and good specificity

Pending Publication Date: 2022-03-01
SUZHOU CHIEN SHIUNG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3,4-methylenedioxyphenylethylamine is a key intermediate in the synthesis reaction process of berberine, and its content directly affects the content and yield of berberine finished products, but there is no relevant literature report on the rapid detection method for its content

Method used

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  • High performance liquid chromatography detection method of berberine intermediate

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Embodiment 1

[0039] A high-performance liquid chromatography detection method for berberine intermediates, comprising the following steps:

[0040] 1) Determine the chromatographic conditions:

[0041] Column: C18;

[0042] Detector: UV detector;

[0043] Mobile phase: Acetonitrile + sodium lauryl sulfate aqueous solution is used as the mobile phase;

[0044] The detection wavelength of the detector is 200nm;

[0045] The volume ratio of acetonitrile and sodium lauryl sulfate aqueous solution in the mobile phase is 50:50;

[0046] The column temperature is 30°C;

[0047] The flow rate is 0.5mL / min;

[0048] Injection volume 5uL;

[0049] Elution method: isoconcentration elution;

[0050] 2) prepare the test solution;

[0051] 3) Preparation of reference substance solution:

[0052] 4) Determination method:

[0053] a. Prepare standard products and calculate the standard curve;

[0054] b. Precisely measure the test solution and the reference solution respectively, inject them in...

Embodiment 2

[0060] A high-performance liquid chromatography detection method for berberine intermediates, comprising the following steps:

[0061] 1) Determine the chromatographic conditions:

[0062] Column: C18;

[0063] Detector: UV detector;

[0064] Mobile phase: Acetonitrile + sodium lauryl sulfate aqueous solution is used as the mobile phase;

[0065] The detection wavelength of the detector is 205nm;

[0066] The volume ratio of acetonitrile and sodium lauryl sulfate aqueous solution in the mobile phase is 50:50;

[0067] The column temperature is 40°C;

[0068] The flow rate is 1mL / min;

[0069] Injection volume 10uL;

[0070] Elution method: isoconcentration elution;

[0071] 2) prepare the test solution;

[0072] 3) Preparation of reference substance solution:

[0073] 4) Determination method:

[0074] a. Prepare standard products and calculate the standard curve;

[0075] b. Precisely measure the test solution and the reference solution respectively, inject them int...

Embodiment 3

[0081] A high-performance liquid chromatography detection method for berberine intermediates, comprising the following steps:

[0082] 1) Determine the chromatographic conditions:

[0083] Column: C18;

[0084] Detector: UV detector;

[0085] Mobile phase: Acetonitrile + sodium lauryl sulfate aqueous solution is used as the mobile phase;

[0086] The detection wavelength of the detector is 210nm;

[0087] The volume ratio of acetonitrile and sodium lauryl sulfate aqueous solution in the mobile phase is 50:50;

[0088] The column temperature is 50°C;

[0089] The flow rate is 1.5mL / min;

[0090] Injection volume 15uL;

[0091] Elution method: isoconcentration elution;

[0092] 2) prepare the test solution;

[0093] 3) Preparation of reference substance solution:

[0094] 4) Determination method:

[0095] a. Prepare standard products and calculate the standard curve;

[0096] b. Precisely measure the test solution and the reference solution respectively, inject them i...

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Abstract

The invention belongs to the field of medical intermediate detection, and particularly discloses a high performance liquid chromatography detection method of berberine intermediates, the intermediates are homopiperony lamine, the chemical name is 3, 4-methylenedioxy phenylethylamine, and the detection method comprises the steps of determination of chromatographic conditions, preparation of a test solution, preparation of a reference solution and preparation of a standard substance. Calculating a standard curve; respectively and precisely measuring the test solution and the reference solution, injecting into a liquid chromatograph, recording a chromatogram, and calculating the content by peak area according to an external standard method. The detection method disclosed by the invention is good in specificity, high in sensitivity and high in separation degree, and durability, linearity and the like can meet requirements.

Description

technical field [0001] The invention belongs to the field of detection of pharmaceutical intermediates, and specifically discloses a high performance liquid chromatography detection method for berberine intermediates. Background technique [0002] Berberine, also known as berberine, is an important antibiotic, which has antibacterial effects on hemolytic streptococcus, Staphylococcus aureus, gonorrhoeae, Freund's, Shigella, etc., and can enhance leukocyte phagocytosis It also has different degrees of inhibitory effects on Mycobacterium tuberculosis and Yersinia pestis, and also has inhibitory effects on amoebic bacteria in rats. Berberine hydrochloride (commonly known as berberine hydrochloride) has been widely used in the treatment of gastroenteritis, bacillary dysentery, etc., and has a certain effect on tuberculosis, scarlet fever, acute tonsillitis and respiratory tract infection. Coptis chinensis, Phellodendron phellodendri, Sangezhen and Shigonggong are commonly used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 郁惠珍苗向阳严丹红
Owner SUZHOU CHIEN SHIUNG INST OF TECH
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