Method for sampling, storing and measuring length of rice anther

Abstract

The invention discloses a method for sampling, storing and measuring the length of rice anther. The method comprises the following steps: providing a rice material, sampling in the full-bloom stage of rice, taking the material back into a room, wrapping the material with a preservative film in time, putting the material into a refrigerator at the temperature of-30 DEG C for cryopreservation, dissecting and photographing, and measuring the length of the rice anther by using Image-ProPlus6.0 software. The method can be used for sampling a large number of materials, can be used for dissecting, photographing and measuring by multiple persons at the same time, is rapid and efficient, and can enable the rice anther to be preserved for a long time and keep a fresh and alive state. The application of the method has important significance on rice anther length genetic research, gene localization and cloning, biological function research and the like.

Description

technical field [0001] The invention belongs to the field of rice flower organ character investigation and data statistics, and in particular relates to a method for sampling, storing and measuring the length of rice anthers. Background technique [0002] In the prior art, in the research on the length of rice anthers, different researchers have different sampling and investigation methods, and there is no uniform standard. Mainly manifested in 2 aspects: [0003] One is that the sampling standards for anther length are not uniform and the measurement methods are diverse, making it difficult to repeat or evaluate the survey results. Zhou Dan (Research on QTL mapping of anther length and seed dormancy in wild rice [D]. Hunan Agricultural University, 2005) took samples at the beginning of heading, took 2 panicles from each plant, and took 3 spikelets from the upper, middle and lower parts of each panicle for investigation. After scanning the anthers, the instrument magnified...

AI Technical Summary

Problems solved by technology

Ogami et al. (Identification of Anther Length QTL and Construction of Chromosome Segment Substitution Lines of Oryza longistaminata.[J].Plants,2019) took samples at the heading stage, took all the anthers of a floret that was about to bloom, placed them on a glass slide, and placed them under a microscope (Axioplan, Carl Zeiss, Jena, Germany) was taken with a digital camera (DMX-1200, Nikon, Tokyo, Japan), and the length of 4-6 anthers was measured with ImageJ software 1.8, and the average anther length was used as the phenotype of a single plant The sampling time is relatively wide, and it is difficult to unify the standard among different operators. It is only suitable for the sampling and measurement of small samples or a small amount of materials, which is not conducive to the investigation of a large number of materials.

[0004] Second, it is difficult to preserve the survey samples, and there is no unified preservation method. When the sample size is not large, most people choose live surveys, that is, take as many as you like, and do not need to store for a long time

The applicant tried to use Carnot's fixative solution and 70% ethanol to fix and preserve the florets, and then dissected the anthers for measurement, but at this time the anthers became soft, and the shape of the anthers, especially the width of the anthers, changed significantly. Difficult to straighten, resulting in unstable measurement results

Images