Construction method of improved mouse ovariectomy operation model

Abstract

The invention discloses a construction method of an improved mouse ovariectomy operation model, which belongs to the technical field of biological modeling, and mainly comprises the following steps: placing a mouse in a prone position, taking the highest point of the back spine of the mouse as a starting point, and cutting a 1cm incision along the midline of the back of the mouse; the lowest point of the side face of the mouse is found from the surgical incision, the position is the mouse posterior peritoneal space, white adipose tissue can be seen through the wall layer peritoneum, and the mouse ovary position is found; performing annular ligation on the joint of the ovary and the fallopian tube by using a No.1 line; the ligated ovary and the ligated ovarian artery are scalded and stopped by using an electrocoagulation pen, and redundant thread residues are cut off; the adipose tissue and the fallopian tube are restored; and closing the incision of the mouse by using a skin stitching instrument. Compared with the prior art, the method has the following advantages: the operation is easy; the infection risk during the operation is effectively reduced; the bleeding amount and the death rate are effectively reduced; surgical wounds are small, and the recovery period can be shortened.

Description

technical field [0001] The invention relates to the technical field of biological modeling, in particular to a method for constructing an improved mouse ovariectomy model. Background technique [0002] Estrogen is a common steroid hormone in the body, mainly produced by the ovaries of female animals. In addition, the placenta, follicle, and corpus luteum of pregnancy can also produce a small amount of estrogen. Estrogen receptors are widely distributed in multiple organs of the body, such as uterus, breast, bladder, urethra, brain and skin. Therefore, estrogen not only has the physiological effect of promoting and maintaining female reproductive organs and secondary sexual characteristics, but also has obvious effects on the endocrine system, cardiovascular system, metabolic system, bone growth and maturation, and skin homeostasis. Play an important regulatory role in the body. In addition, the imbalance of estrogen regulation is also closely related to the occurrence and...

AI Technical Summary

Problems solved by technology

[0007] Disadvantages: The abdominal incision is far away from the ovary, and the operation needs to be entered from the front abdomen, which may cause damage to other abdominal organs

[0009] Disadvantages: double incision on the back, long incision, increased risk of infection and bleeding, and the location of the double incision on the ovary is not clear, and because the skin incision and deep incision are in the same position, the risk of postoperative infection is higher, which is not good for subsequent recovery

Open wounds are easy to cause postoperative infection in mice, and fresh wounds can cause blood loss in mice. The above factors are all potential risks that cause data instability between mouse models, increased mouse mortality, and unsuccessful surgery.

Images