Human umbilical cord mesenchymal stem cell culture medium, human umbilical cord mesenchymal stem cell injection, preparation method and application of human umbilical cord mesenchymal stem cell injection in preparation of medicine for treating cerebral apoplexy

A technology of mesenchymal stem cells and human umbilical cord, applied in the direction of cell culture active agents, biochemical equipment and methods, animal cells, etc., can solve the problems of limited treatment strategies and affect the treatment effect, to facilitate mass spectrometry detection, promote proliferation ability, and promote proliferating effect

Pending Publication Date: 2022-03-11
SHANGHAI AISAER BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current therapeutic strategies based on mesenchymal stem cells, especially those based on umbilical cord mesenchymal stem cells, are still very limited.
Moreover, there are certain differences in the cell proliferation ability of umbilical cord mesenchymal stem cells obtained under different culture conditions, which also directly affects its therapeutic effect.

Method used

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  • Human umbilical cord mesenchymal stem cell culture medium, human umbilical cord mesenchymal stem cell injection, preparation method and application of human umbilical cord mesenchymal stem cell injection in preparation of medicine for treating cerebral apoplexy
  • Human umbilical cord mesenchymal stem cell culture medium, human umbilical cord mesenchymal stem cell injection, preparation method and application of human umbilical cord mesenchymal stem cell injection in preparation of medicine for treating cerebral apoplexy
  • Human umbilical cord mesenchymal stem cell culture medium, human umbilical cord mesenchymal stem cell injection, preparation method and application of human umbilical cord mesenchymal stem cell injection in preparation of medicine for treating cerebral apoplexy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] This embodiment provides a method for culturing human umbilical cord mesenchymal stem cells

[0066] (1) Prepare serum-free medium according to the following formula. There is no strict order for the addition of various components, and it can be operated at room temperature. The preparation process does not need to be heated, and it is carried out under a biological safety cabinet. After the preparation is completed, use a 0.22 μm filter. filter.

[0067] The formula is: DMEM / F12 medium, platelet-derived growth factor (PDGF) 20ng / ml, epidermal growth factor (EGF) 15ng / ml, vitamin C 25μg / ml, insulin 12μg / ml, β-mercaptoethanol 10ng / ml, SAG 7μmol / L, folic acid 5.5mg / L, hypoxia-inducible factor-1 (HIF-1) 15ng / ml, cobalt protoporphyrin (CoPP) 50μmol / L, basic fibroblast growth factor (bFGF) 10mg / ml , Simvastatin 10 μg / ml.

[0068] (2) Shred the isolated Wharton's jelly with surgical scissors, put it in a collagenase solution with a concentration of 0.05U / ml for digestion fo...

Embodiment 2

[0070] This embodiment provides a method for culturing human umbilical cord mesenchymal stem cells

[0071] (1) Prepare serum-free medium according to the following formula. There is no strict order for the addition of various components, and it can be operated at room temperature. The preparation process does not need to be heated, and it is carried out under a biological safety cabinet. After the preparation is completed, use a 0.22 μm filter. filter.

[0072]The formula is: F12 medium, platelet-derived growth factor (PDGF) 12ng / ml, epidermal growth factor (EGF) 25ng / ml, vitamin C 20μg / ml, insulin 15μg / ml, β-mercaptoethanol 6ng / ml, SAG 10μmol / L, folic acid 3.5mg / L, hypoxia-inducible factor-1 (HIF-1) 18ng / ml, cobalt protoporphyrin (CoPP) 30μmol / L, basic fibroblast growth factor (bFGF) 15mg / ml, octyl Vastatin 6 μg / ml.

[0073] (2) Cut the isolated Wharton's jelly into pieces with surgical scissors, put it in a collagenase solution with a concentration of 0.06U / ml for digest...

Embodiment 3

[0075] This embodiment provides a method for culturing human umbilical cord mesenchymal stem cells

[0076] (1) Prepare serum-free medium according to the following formula. There is no strict order for the addition of various components, and it can be operated at room temperature. The preparation process does not need to be heated, and it is carried out under a biological safety cabinet. After the preparation is completed, use a 0.22 μm filter. filter.

[0077] The formula is: DMEM / F12 medium, platelet-derived growth factor (PDGF) 25ng / ml, epidermal growth factor (EGF) 10ng / ml, vitamin C 30μg / ml, insulin 10μg / ml, β-mercaptoethanol 12ng / ml, SAG 6μmol / L, folic acid 7mg / L, hypoxia-inducible factor-1 (HIF-1) 12ng / ml, cobalt protoporphyrin (CoPP) 70μmol / L, basic fibroblast growth factor (bFGF) 7mg / ml, Simvastatin 13 μg / ml.

[0078] (2) Cut the isolated Wharton's jelly with surgical scissors, place it in a collagenase solution with a concentration of 0.05U / ml for digestion for 3h...

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Abstract

The invention provides a human umbilical cord mesenchymal stem cell culture medium, a human umbilical cord mesenchymal stem cell injection, a preparation method and application of the human umbilical cord mesenchymal stem cell culture medium and the human umbilical cord mesenchymal stem cell injection in preparation of drugs for treating cerebral apoplexy. The human umbilical cord mesenchymal stem cell culture medium comprises a basic culture medium, vitamin C, insulin, platelet-derived growth factors, epidermal growth factors, basic fibroblast growth factors, beta-mercaptoethanol, SAG, folic acid, hypoxia inducible factor-1, protoporphyrin cobalt and simvastatin. The SAG, the hypoxia inducible factor-1, the protoporphyrin cobalt and the simvastatin are creatively combined to be used for preparing the human umbilical cord mesenchymal stem cell culture medium, and the four components are matched with one another, so that the human umbilical cord mesenchymal stem cell culture medium has a remarkable synergistic interaction effect in the aspect of promoting proliferation of umbilical cord mesenchymal stem cells. The human umbilical cord mesenchymal stem cells obtained through culture are high in multiplication capacity, the prepared human umbilical cord mesenchymal stem cell injection can be directly used for treating cerebral apoplexy after resuscitation, and the human umbilical cord mesenchymal stem cell injection has important application value.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and relates to a human umbilical cord mesenchymal stem cell culture medium and its application, in particular to a human umbilical cord mesenchymal stem cell culture medium, an injection, a preparation method and its use in preparing a medicine for treating cerebral apoplexy Applications. Background technique [0002] Mesenchymal stem cells (MSCs) are pluripotent stem cells with high self-renewal ability and multi-lineage differentiation potential derived from the early mesoderm of development. They are widely present in various tissues throughout the body and can be cultured and expanded in vitro. And can differentiate into nerve cells, osteoblasts, chondrocytes, muscle cells and fat cells under specific conditions. With the in-depth study of the biological characteristics and functions of mesenchymal stem cells, MSCs have been isolated from various tissues. MSCs are ideal cells for cell ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/28A61K9/08A61P9/10
CPCC12N5/0668A61K35/28A61K9/08A61K9/0019A61P9/10C12N2500/38C12N2501/135C12N2501/11C12N2501/115C12N2500/44C12N2501/33C12N2500/90C12N2501/999C12N2501/998
Inventor 郝佳丽高歌
Owner SHANGHAI AISAER BIOTECH CO LTD
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