Multi-strain compound fermentation method of fresh and tender corn stigma

A composite fermentation, corn silk technology, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc.

Pending Publication Date: 2022-03-15
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Mixed fermentation can obtain some unique produc

Method used

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  • Multi-strain compound fermentation method of fresh and tender corn stigma
  • Multi-strain compound fermentation method of fresh and tender corn stigma
  • Multi-strain compound fermentation method of fresh and tender corn stigma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of a kind of fresh and tender corn silk multi-species composite fermentation liquid is as follows:

[0031] The first step: Pre-treatment of fresh and tender corn silk: rinse and dry, crush through a 60-mesh sieve, and corn silk powder is subjected to five-step treatment at a material-to-liquid ratio of 1:10, 1:15, 1:20, 1:25, and 1:30, respectively. Groups of parallel experiments were subjected to reflux extraction at 90°C for 2 hours.

[0032] Step 2: After the extract is cooled, it is filtered twice, centrifuged at 4000r for 10 minutes to take the supernatant, and the supernatant is suction-filtered once; the filtrate is sterilized under high temperature and high pressure at 121°C for 15 minutes; the sterile solution is cooled to room temperature.

[0033] Step 3: insert logarithmic Saccharomyces cerevisiae and Lactobacillus paracasei into the aseptic solution to ferment. Among them, the inoculation amount of the composite strain is 8%; the ra...

Embodiment 2

[0038] The preparation method of a kind of fresh and tender corn silk multi-species composite fermentation liquid is as follows:

[0039] Step 1: Pre-treatment of fresh and tender corn silk: rinse and dry, crush through a 60-mesh sieve, and extract the corn silk powder at 90°C for 2 hours at a solid-to-liquid ratio of 1:15.

[0040] Step 2: After the extract is cooled, it is filtered twice, centrifuged at 4000r for 10 minutes to take the supernatant, and the supernatant is suction-filtered once; the filtrate is sterilized under high temperature and high pressure at 121°C for 15 minutes; the sterile solution is cooled to room temperature.

[0041] The third step: adding logarithmic Saccharomyces cerevisiae and Lactobacillus paracasei to the aseptic solution for fermentation. The aseptic solution was divided into five groups, and parallel experiments were carried out, wherein the inoculum amount of the composite strains was 4%, 6%, 8%, 10%, and 12% respectively; the ratio of the...

Embodiment 3

[0046] The preparation method of a kind of fresh and tender corn silk multi-species composite fermentation liquid is as follows:

[0047] Step 1: Pre-treatment of fresh and tender corn silk: rinse and dry, crush through a 60-mesh sieve, and extract the corn silk powder at 90°C for 2 hours at a solid-to-liquid ratio of 1:15.

[0048] Step 2: After the extract is cooled, it is filtered twice, centrifuged at 4000r for 10 minutes to take the supernatant, and the supernatant is suction-filtered once; the filtrate is sterilized under high temperature and high pressure at 121°C for 15 minutes; the sterile solution is cooled to room temperature.

[0049]The third step: adding logarithmic Saccharomyces cerevisiae and Lactobacillus paracasei to the aseptic solution for fermentation. Among them, the inoculum amount of the compound strain was 8%; the proportions of the compound strains of Saccharomyces cerevisiae and Lactobacillus paracasei were respectively 1:1, 1:2, 1:3, 2:1, and 3:1 in...

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Abstract

The invention discloses a multi-strain compound fermentation method of fresh and tender corn stigma, which comprises the following steps: (1) pretreatment: rinsing and drying the fresh and tender corn stigma, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction on corn stigma powder for 2 hours at 90 DEG C, cooling an extracting solution, filtering, centrifuging for 10 minutes at 4000 r, taking supernate, carrying out suction filtration on the supernate, carrying out high-temperature and high-pressure sterilization on filtrate for 15 minutes at 121 DEG C, and then cooling a sterile solution to room temperature; (2) fermentation: inoculating lactobacillus paracasei and saccharomyces cerevisiae in a logarithmic phase into the sterile liquid obtained in the step (1) for compound fermentation, wherein the inoculum size of a compound strain is 8%; and (3) storage: placing the fermentation liquor at 4 DEG C for after-ripening for 12 hours, and centrifuging to take supernate. According to the multi-strain compound fermentation method for the fresh and tender corn stigma, after the fresh and tender corn stigma is treated by a multi-strain compound fermentation technology, the activity of inhibiting alpha-glucosidase on chorion of small intestine epithelial cells is improved, the absorption of a human body to glucose is delayed, and the increase of postprandial blood sugar is controlled; the prevention and treatment of type II diabetes and complications thereof are facilitated.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a multi-strain composite fermentation method for fresh and tender corn silk. Background technique [0002] Diabetes mellitus is an endocrine and metabolic disease characterized by hyperglycemia, which can lead to a series of metabolic disorders such as sugar, protein, and fat caused by hypofunction of pancreatic islets and insulin resistance. Diabetic patients mostly have symptoms such as polydipsia, polyphagia, polyuria, weight loss, and general weakness. Type Ⅱ diabetes accounts for more than 90% of the diabetic population. α-glucosidase inhibitors inhibit the activity of α-glucosidase on the chorionic epithelial cells of the small intestine, delay the absorption of glucose by the human body, control the rise of blood sugar after meals, and help prevent and treat type Ⅱ diabetes and its complications . In recent years, the screening of natural α-glucosidase i...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12P1/04C12P39/00C12R1/225C12R1/865
CPCC12P1/02C12P1/04C12P39/00
Inventor 郑喜群包影刘晓兰姜彩霞
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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