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Veterinary Shuanghuanglian oral liquid quality detection method based on spectrum-activity relationship

A technology of Shuanghuanglian oral liquid and quality detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of less research on the spectrum-effect relationship of veterinary Shuanghuanglian oral liquid, complex and time-consuming methods, and achieve shortened detection Time, strong objectivity, less costly effect

Pending Publication Date: 2022-03-15
RINGPU TIANJIN BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, two methods are used in the current veterinary pharmacopoeia standards to realize the quantitative detection of baicalin, chlorogenic acid and forsythin, the main components of the three medicinal materials in Shuanghuanglian oral liquid, respectively. The method is complicated and time-consuming; There are few studies on the spectrum-effect relationship of veterinary Shuanghuanglian oral liquid. Therefore, the research methods for the quality control of veterinary Shuanghuanglian oral liquid need to be further explored.

Method used

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  • Veterinary Shuanghuanglian oral liquid quality detection method based on spectrum-activity relationship
  • Veterinary Shuanghuanglian oral liquid quality detection method based on spectrum-activity relationship
  • Veterinary Shuanghuanglian oral liquid quality detection method based on spectrum-activity relationship

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Establishment of HPLC Fingerprint of Shuanghuanglian Oral Liquid for Animals

Embodiment approach

[0065] 1. Analysis conditions

[0066] Instrument: Waters Alliance HPLC

[0067] Chromatographic column: Cosmosil C18-5μm-250mm

[0068] Flow rate: 1.0mL / min

[0069] Column temperature: 35°C

[0070] Injection volume: 10μL

[0071] Wavelength collection range: 190nm~600nm

[0072] Wavelength: 277nm

[0073] Mobile phase: A. Acetonitrile: B. 0.1% formic acid / water (v / v)

[0074] gradient:

[0075] time (min) A(％) B(％) 0～15 8→15 92→85 15～25 15→15 85→85 25～45 15→35 85→65 45～50 35→80 65→20 50～60 80→80 20→20

[0076] 2. Preparation of reference substance: take an appropriate amount of chlorogenic acid reference substance, weigh it accurately, add 50% methanol / water to make a stock solution containing 100 μg of chlorogenic acid per 1 ml; take an appropriate amount of caffeic acid reference substance, weigh it accurately, add 50% methanol / water to make a stock solution containing 200 μg of caffeic acid per 1 ml; take an appropria...

Embodiment 2

[0079] Embodiment 2 methodological verification

[0080] 1. Instrument precision verification

[0081] According to the sample preparation method and chromatographic conditions of Example 1, a portion of the test solution was prepared and injected continuously for six times. Taking baicalin (peak 4) as the reference peak, the characteristic peaks (peak 1: chlorogenic acid; peak 2: caffeic acid; peak 3: rutin; peak 4: baicalin; peak 5: forsythin) and reference peaks were investigated The relative retention times (Table 1) and relative peak area values ​​(Table 2).

[0082] The results showed that the same test sample of Shuanghuanglian Oral Liquid for Veterinary Injection had six consecutive injections, the RSD of the relative retention time of each characteristic peak was between 0.093% and 0.923%, and the RSD of the relative peak area was between 0.007% and 0.015%. It shows that the precision of the instrument used is good.

[0083] Table 1: Relative retention time RSD (ex...

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Abstract

The invention discloses a method for detecting quality of Shuanghuanglian of veterinary Shuanghuanglian oral liquid based on a spectrum-activity relationship. The method comprises the following steps: (1) establishing an HPLC fingerprint spectrum of the veterinary Shuanghuanglian oral liquid; (2) extracting characteristic chromatographic peaks; (3) determining the anti-inflammatory effect of the veterinary Shuanghuanglian oral liquid; and (4) establishing spectrum-activity correlation analysis by adopting a grey correlation analysis method so as to evaluate the anti-inflammatory activity of the characteristic peak. The method has the advantages of simplicity, high objectivity and the like, and is beneficial to quality control of the veterinary Shuanghuanglian oral liquid.

Description

technical field [0001] The invention belongs to the technical field of drug detection, in particular to a method for detecting the quality of veterinary Shuanghuanglian oral liquid based on spectrum-effect relationship. [0002] technical background [0003] Veterinary Shuanghuanglian Oral Liquid is composed of Scutellaria baicalensis, honeysuckle and forsythia at a ratio of 1:1:2. Honeysuckle is the dry flower bud or the first bloomed flower of Lonicera Lonicerae. It is the monarch drug in the formula. It uses chlorogenic acid compounds, volatile oils, flavonoids, etc. as the main active substances. It has antibacterial, anti-inflammatory, antiviral, Antipyretic and other effects; Forsythia is the dried fruit of Forsythia oleaceae plant, which is the ministerial drug in the prescription, with lignans such as forsythin, phenylethanol glycoside forsythiaside A, flavonoid rutin, etc. As the main chemical component, it has the effects of clearing away heat and detoxification, r...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 李虹颖何睿王一凡廖茂梁刘心怡刘爱玲任连泉李守军史德胜
Owner RINGPU TIANJIN BIOLOGICAL PHARMA
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