Nanoelectrode biosensor based on nucleic acid aptamer and application of nanoelectrode biosensor
A nucleic acid aptamer and biosensor technology, which is applied in biochemical equipment and methods, microbial determination/inspection, instruments, etc. Concentration characterization, etc.
Active Publication Date: 2022-03-18
TSINGHUA UNIV
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Problems solved by technology
For some biomarkers, the current technology has not achieved high-sensitivity detection and concentration characterization, and cannot meet the needs of diagnosis and prevention of certain diseases, especially early diseases
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[0052] The experimental methods used in the following examples are conventional methods unless otherwise specified.
[0053] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
[0054] Such as figure 1 As shown, it is a schematic structural diagram of the nano-electrode biosensor provided by the present invention, including a nano-electrode chip 1 and a drainage module 2 . The nano-electrode chip 1 includes a silicon substrate 1-1, a gold nanowire 1-4 and a signal collection electrode 1-3 disposed on the silicon substrate 1-1, and the two ends of the gold nanowire 1-4 are connected to the signal collection electrode 1-3 , a silicon dioxide insulating layer 1-2 is grown on the surface of the silicon substrate 1-1.
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Abstract
The invention discloses a nanoelectrode biosensor based on a nucleic acid aptamer and application of the nanoelectrode biosensor. The nanoelectrode biosensor based on the nucleic acid aptamer comprises a nanoelectrode chip, the nano electrode chip comprises a substrate, and a metal nanowire and a signal acquisition electrode which are arranged on the substrate; two ends of the metal nanowire are connected with the signal acquisition electrode; a nucleic acid aptamer is modified on the metal nanowire to serve as a detection probe; the nucleic acid aptamer is single-stranded DNA or single-stranded RNA, and the length of the nucleotide sequence is 20-80 nt. By using the manufacturing method provided by the invention, the metal nanoelectrode sensor chip can be rapidly prepared, the nucleic acid aptamer probe which can be specifically combined with a specific protein or small molecule biomarker is adopted, and the probe is fixed on the surface of the metal nanoelectrode, so that the biomarker can be detected. The excellent electrical property of the metal nanoelectrode is combined with the high binding capacity and high specificity advantage of the nucleic acid aptamer, and high-sensitivity detection is carried out on the target biomarker.
Description
technical field [0001] The invention relates to a nano-electrode biosensor based on a nucleic acid aptamer and an application thereof, belonging to the technical field of biological testing. Background technique [0002] Quantitative detection of trace biomarkers is of great significance for the early diagnosis and prognosis of cancer, cardiovascular and other major diseases. For example, the detection of cardiac troponin I (cTnI) is used in the clinical diagnosis of acute myocardial infarction (AMI), and plasma cTnI at a level as low as 0.01 ng / mL can predict the occurrence of chronic heart failure (CHF). At present, the detection of biomarkers in clinical applications is mainly carried out through the analysis of body fluid and excrement samples by means of detection methods such as large-scale medical testing instruments or kits. For some biomarkers, the current technology has not achieved high-sensitivity detection and concentration characterization, and cannot meet the...
Claims
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IPC IPC(8): G01N27/327C12Q1/6825
CPCG01N27/3275G01N27/3277G01N27/3278C12Q1/6825
Inventor 王晗张俊峰孙凯
Owner TSINGHUA UNIV