Method for preparing block copolymer
A block copolymer, ring-opening polymerization technology, applied in biochemical equipment and methods, using vectors to introduce foreign genetic material, transferase, etc., can solve problems such as molecular weight limitations, achieve excellent mechanical properties, and maintain environmental compatibility and biodegradability effects
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[0055] In the method for preparing a block copolymer according to one embodiment, based on 100 mol% of the lactide monomer, the content of the catalyst may be 0.01 mol% to 10 mol%, 0.05 mol% to 8 mol%, 0.07 mol% to 5 mole % or 0.09 mole % to 3 mole %. If the content of the catalyst is less than 0.01 mol% with respect to 100 mol% of the lactide monomer, the polymerization activity will be insufficient, and if the content of the catalyst exceeds 10 mol%, the prepared polylactide-poly(3-hydroxylactide (acid ester) block copolymer may increase in the amount of residual catalyst, cause decomposition of the copolymer or decrease in molecular weight due to depolymerization such as transesterification.
[0056]Ring-opening polymerization may be performed at 150°C to 200°C for 5 minutes to 10 hours.
[0057] In addition, the ring-opening polymerization reaction can be carried out by bulk polymerization substantially without using a solvent. In this regard, the use of substantially no...
Embodiment 1 to Embodiment 4
[0062] (1) Biosynthesis of poly(3-hydroxypropionate) oligomers
[0063] 1) Preparation of high molecular weight poly(3-hydroxypropionate)
[0064] For the biosynthesis of high-molecular-weight poly(3-hydroxypropionate) according to the present disclosure, first, fermentation was performed based on the following conditions in order to prepare a fermentation broth containing 3-hydroxypropionate as a fermentation substrate. Specifically, Escherichia coli W3110 having GDH and ALDH enzyme genes was used as a strain for fermentation. Using M9 as a medium, and using 70 g / L of glycerol as a substrate, fermentation was performed to produce 3-hydroxypropionate.
[0065] After that, using the 3-hydroxypropionate prepared above as a substrate, fermentation was performed and P(3HP) was produced. For the fermentation, specifically, a 5 L fermentation apparatus (inner volume: 3 L) was used. Mutations of the RecC gene, polyhydroxyalkanoate synthase (PHA synthase) from Ralstonia eutropha, a...
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