Wild chrysanthemum flower active site capable of regulating epigenetic expression balance and resisting liver cancer as well as preparation method and application of wild chrysanthemum flower active site

An active site and epigenetic technology, applied in the field of wild chrysanthemum active site and its preparation, to achieve the effect of reducing oxidative stress damage

Active Publication Date: 2022-03-22
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the current research on anti-tumor drugs targeting epigenetic targets has become a hot spot, but the drugs approved for marketing are all chemical drugs, and they are aimed at a single target, such as inhibitors of DNA methylase DNMT1

Method used

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  • Wild chrysanthemum flower active site capable of regulating epigenetic expression balance and resisting liver cancer as well as preparation method and application of wild chrysanthemum flower active site
  • Wild chrysanthemum flower active site capable of regulating epigenetic expression balance and resisting liver cancer as well as preparation method and application of wild chrysanthemum flower active site
  • Wild chrysanthemum flower active site capable of regulating epigenetic expression balance and resisting liver cancer as well as preparation method and application of wild chrysanthemum flower active site

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of the active part of the wild chrysanthemum that has the effect of regulating epigenetic expression and balancing anti-liver cancer in this embodiment is as follows: use 65% ethanol as a solvent, ultrasonically extract, concentrate under reduced pressure, remove ethanol to obtain the wild chrysanthemum extract, add pure Adjust the water to a specific gravity of about 1, extract through a gradient of petroleum ether-ethyl acetate-ethanol (5:1:0.1), and remove impurities by silica gel column chromatography (the mobile phase is petroleum ether-ethyl acetate-ethanol (10:1:0.5 ) Gradient elution) to obtain the active site (YJH-B).

[0027] Among them, the quality control of active parts: total sesquiterpene content ≥ 50%, total flavonoid content ≥ 30%.

[0028] Index components: ragnidin A content ≥ 4.0%, chrysanthemum lactone content ≥ 3.0%, apigenin content ≥ 0.5%, acacinin content ≥ 0.7%.

Embodiment 2

[0030] The preparation method of the active part of the wild chrysanthemum that has the effect of regulating epigenetic expression and balancing anti-liver cancer in this embodiment is as follows:

[0031] Use 80% ethanol as a solvent, ultrasonically extract, concentrate under reduced pressure, and remove all ethanol to obtain wild chrysanthemum extract, add purified water to adjust the specific gravity to about 1.1, and pass through petroleum ether-ethyl acetate-ethanol (1:8:2) Gradient extraction, silica gel column chromatography to remove impurities (mobile phase is petroleum ether-ethyl acetate-ethanol (10:5:2) gradient elution) to obtain the active site (YJH-B).

[0032] Among them, the quality control of active parts: total sesquiterpene content ≥ 50%, total flavonoid content ≥ 30%.

[0033] Index components: ragnidin A content ≥ 4.0%, chrysanthemum lactone content ≥ 3.0%, apigenin content ≥ 0.5%, acacinin content ≥ 0.7%.

Embodiment 3

[0035] The preparation method of the active part of the wild chrysanthemum that has the effect of regulating epigenetic expression and balancing anti-liver cancer in this embodiment is as follows:

[0036] Use 70% ethanol as a solvent, ultrasonically extract, concentrate under reduced pressure, and remove all ethanol to obtain wild chrysanthemum extract, add purified water to adjust the specific gravity to about 1.2, and pass through petroleum ether-ethyl acetate-ethanol (5:20:6) Gradient extraction, silica gel column chromatography to remove impurities (mobile phase is petroleum ether-ethyl acetate-ethanol (10:3:1.5) gradient elution) to obtain the active site (YJH-B).

[0037] Among them, the quality control of active parts: total sesquiterpene content ≥ 50%, total flavonoid content ≥ 30%.

[0038] Index components: ragnidin A content ≥ 4.0%, chrysanthemum lactone content ≥ 3.0%, apigenin content ≥ 0.5%, acacinin content ≥ 0.7%.

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Abstract

The invention belongs to the field of biological pharmacy, and relates to application of a traditional Chinese medicine wild chrysanthemum active component, in particular to a wild chrysanthemum active part with the effect of regulating epigenetic expression balance and resisting liver cancer as well as a preparation method and application of the wild chrysanthemum active part. The content of total sesquiterpenes in the wild chrysanthemum flower active part is more than or equal to 50%, and the content of total flavonoids is more than or equal to 30%; the index components comprise greater than or equal to 4.0% of ambrotin A, greater than or equal to 3.0% of wild chrysanthemum lactone, greater than or equal to 0.5% of apigenin and greater than or equal to 0.7% of acacetin. According to the invention, an active site (YJH-B) with anti-liver cancer and liver protection effects is extracted and separated from wild chrysanthemum flower for the first time, and it is found for the first time that the molecular mechanism of the action of the active site is realized by regulating the balance relationship of DNMTs/TET2, so that the idea of eliminating pathogens and strengthening body resistance of traditional Chinese medicines for treating cancers is shown; and a new thought and a new strategy are provided for research and development of innovative anticancer drugs based on epigenetic targets.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to the application of the active components of the traditional Chinese medicine chrysanthemum chrysanthemum, in particular to the active part of chrysanthemum chrysanthemum with the function of regulating epigenetic expression balance and anti-liver cancer, as well as its preparation method and application. Background technique [0002] Liver cancer is one of the most common malignant tumors, its incidence ranks fifth among all malignant tumors, and its mortality rate ranks third among all malignant tumors. Cancer is a disease with complex pathogenesis, and the therapeutic effect of single-target drugs on the market is not ideal. The multi-component, multi-path, and multi-target effects of traditional Chinese medicine have certain advantages and characteristics for the prevention and treatment of complex diseases such as cancer. [0003] In recent years, studies have shown that epige...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/287A61P1/16A61P35/00
CPCA61K36/287A61P1/16A61P35/00A61K2236/333A61K2236/35Y02A50/30
Inventor 毕跃峰张芳平刘藤藤白喆
Owner ZHENGZHOU UNIV
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