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Preparation method of shell-core structure nanofiber membrane containing antibacterial peptide

A technology of nanofiber membranes and antimicrobial peptides, which is applied in the field of preparation of nanofiber membranes with a shell-core structure, can solve the problems of low antibacterial activity, easy hydrolysis, and poor stability, and achieve stable release, long-lasting release, and improved barrier properties.

Pending Publication Date: 2022-03-25
天津市口腔医院(天津市整形外科医院南开大学口腔医院) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antibacterial activity of natural antimicrobial peptides is relatively low, and they are easily hydrolyzed, and their stability is poor when used in vivo

Method used

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  • Preparation method of shell-core structure nanofiber membrane containing antibacterial peptide
  • Preparation method of shell-core structure nanofiber membrane containing antibacterial peptide
  • Preparation method of shell-core structure nanofiber membrane containing antibacterial peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of preparation method of the shell-core structure nanofiber membrane containing antimicrobial peptide of the present invention, comprises the following steps:

[0026] (1) Take out 1.5g of PLGA powder from the refrigerator at -20°C and place it at room temperature, dissolve the PLGA powder in 8.5g of DMF solution, stir and dissolve for 20s with a magnetic stirrer, the magnetic stirrer speed is 1000rpm / s, statically Set aside for 24 hours for defoaming to obtain a shell layer PLGA / DMF solution with a concentration of 15 wt%, and store the shell layer PLGA / DMF solution at 4°C for later use;

[0027] (2) Take out 15mg of KSL-W powder from the refrigerator at -20°C and place it at room temperature, mix 4mL of KSL-W powder with distilled water to obtain a core KSL-W aqueous solution with a concentration of 3.75mg / mL, and place Store at 4°C for later use;

[0028] (3) The syringes of the coaxial electrospinning machine each draw 5mL of the shell layer PLGA / DMF solutio...

Embodiment 2

[0037] A kind of preparation method of the shell-core structure nanofiber membrane containing antimicrobial peptide of the present invention, comprises the following steps:

[0038] (1) Take out 1.2g of PLGA powder from the refrigerator at -20°C and place it at room temperature, dissolve the PLGA powder in 8.8g of DMF solution, and stir and dissolve for 20s with a magnetic stirrer, the speed of the magnetic stirrer is 1000rpm / s, And let it stand for 24 hours for defoaming to obtain a shell layer PLGA / DMF solution with a concentration of 14wt%, and store the shell layer PLGA / DMF solution at 4°C for later use;

[0039] (2) Take out 15 mg of KSL-W powder from the refrigerator at -20°C and place it at room temperature, mix the KSL-W powder with 4 mL of distilled water to obtain a core layer KSL-W aqueous solution with a concentration of 3.75 mg / mL, and stored at 4°C for later use;

[0040] (3) The syringes of the coaxial electrospinning machine each draw 5mL of the shell layer PL...

Embodiment 3

[0043] A kind of preparation method of the shell-core structure nanofiber membrane containing antimicrobial peptide of the present invention, comprises the following steps:

[0044] (1) Take out 1.0g of PLGA powder (molecular weight: 40w) from the refrigerator at -20°C and place it at room temperature, dissolve the PLGA powder in 9.0g of DMF solution, and stir and dissolve it with a magnetic stirrer for 20s. 1000rpm / s, then let it stand for 24h to defoam, and obtain a shell layer PLGA / DMF solution with a concentration of 10wt%, and store the shell layer PLGA / DMF solution at 4°C for future use;

[0045] (2) Take out 15mg of KSL-W powder from the refrigerator at -20°C and place it at room temperature, mix the KSL-W powder with 4mL of distilled water to obtain a core layer KSL-W aqueous solution with a concentration of 3.75mg / mL, and Store at 4°C for later use;

[0046] (3) The syringes of the coaxial electrospinning machine each draw 5mL of the shell layer PLGA / DMF solution and...

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Abstract

The invention discloses a preparation method of a shell-core structure nanofiber membrane containing antibacterial peptide, which comprises the following steps: dissolving PLGA (poly (lactic-co-glycolic acid)) powder in a DMF (dimethyl formamide) solution, stirring and dissolving to obtain a shell PLGA / DMF solution with the concentration of 10wt%-18wt% for later use; mixing KSL-W powder with 4-5 mL of distilled water to obtain a core layer KSL-W aqueous solution for later use; respectively absorbing the PLGA / DMF solution of the shell layer and the KSL-W aqueous solution of the core layer by an electrostatic spinning machine to form a nanofiber membrane; and collecting the nanofiber membrane with the thickness of 0.1-0.3 mm, folding the nanofiber membrane, and putting the folded nanofiber membrane into a drying oven at the temperature of 40-70 DEG C for drying to obtain the shell-core structure nanofiber membrane containing the antibacterial peptide. By utilizing a coaxial electrostatic spinning technology, the barrier performance of the fiber membrane is ensured, the mechanical property is also improved, the antibacterial peptide enables the nanofiber membrane to have antibacterial property, and the defect that the prognosis is influenced due to the fact that an existing membrane material is easily subjected to bacterial infection with the outside world is overcome; antibacterial drugs are effectively released in a lesion area, the tissue affinity of the multi-layer fiber membrane is improved, and meanwhile, the initial burst release phenomenon of the drugs is reduced.

Description

technical field [0001] The invention belongs to the technical field of periapical periodontitis repair materials, and in particular relates to a preparation method of a shell-core structure nanofiber membrane containing antimicrobial peptides. Background technique [0002] Apical periodontitis is a common oral disease caused by periapical tissue inflammation and bone destruction caused by microbial infection in the dental pulp. Clinically, root canal treatment is commonly used to remove the infection in the dental pulp and promote the healing of the lesion. Modern root canal treatment technology has become more and more mature, but there are still some affected teeth whose alveolar bone destruction has not stopped after treatment. [0003] With the continuous development of endodontic treatment techniques, regenerative techniques (such as guided regeneration of bone / tissue regeneration, etc.) are increasingly used in the treatment of a wide range of periapical bone defects. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/06A61L31/04A61L31/14A61L31/16D04H1/4382D04H1/728
CPCA61L31/06A61L31/047A61L31/16A61L31/14D04H1/728D04H1/43828D04H1/43838A61L2300/252A61L2300/404A61L2400/12C08L67/04
Inventor 申静李瑞欣徐小茵李聪高静索来乐鑫邹慧儒颜艳王冠华刘国昌郭春刚
Owner 天津市口腔医院(天津市整形外科医院南开大学口腔医院)
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