Tb/CdTe ratiometric fluorescent probe, preparation method and application of Tb/CdTe ratiometric fluorescent probe in norfloxacin detection
A ratiometric fluorescent probe, norfloxacin technology, applied in the field of antibiotic detection, high sensitivity detection, and rapid detection, to achieve great application potential, fast response speed, good sensitivity and selectivity.
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Embodiment 1
[0031] Preparation of Red Luminescent CdTe Quantum Dots and Tb / CdTe
[0032] 1a, first, 0.0272g (0.1mmol) Cd (CH 3 CO 2 ) 2 2H 2 O was dissolved in 50 ml of deionized water, the pH was adjusted to 10.5 with 1M NaOH solution, and the mixture was heated and stirred until boiling. Subsequently, 0.0052 g (0.02 mmol) K was added to the above solution 2 TeO 3 , then add 50ml of deionized water and a certain amount of aqueous acetate solution, and then stir for 10 minutes. Quickly add 0.04 g NaBH to the solution 4 and stir for 10 minutes, then heat the mixture until boiling. Finally, the resulting solution was refluxed for 20 hours to obtain CdTe quantum dots. The obtained CdTe quantum dots were placed in a vacuum oven at 60 °C for 12 hours. Collect the dry powder and store it in a refrigerator at 4 °C for future use;
[0033] 1b. Tb / CdTe quantum dots were synthesized by chelation reaction, and the carboxyl groups on the surface of CdTe quantum dots can be combined with Tb ...
Embodiment 2
[0035] 1. Characterization of components in ratiometric fluorescence system
[0036] Considering that the detection sensitivity of probes to analytes is related to their own properties, the structural features and spectral properties of Tb / CdTe probes were studied by FT-IR, UV-vis and fluorescence spectroscopy, respectively. Furthermore, TEM was used to determine the morphology of CdTe and Tb / CdTe probes as well as their dispersibility. As shown in the picture.
[0037] 2. The response of each component of the ratiometric fluorescence system to NFX
[0038] Since the detection of the analyte by the fluorescent probe is related to its fluorescence intensity, the fluorescence intensity of NFX is not the highest under 365nm excitation, but since the eyes are very sensitive to red, CdTe has enough fluorescence brightness to be used as the internal standard fluorescence. Through proper proportioning, a dual-emission ratio fluorescent probe is prepared, which emits red fluorescenc...
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