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Sample cell for terahertz spectrum detection, method for evaluating antibody titer and application

A technology for spectral detection and antibody titer, applied in the field of terahertz waves, can solve the problems of low efficiency and large amount of samples, and achieve the effects of high efficiency, low dosage and high detection accuracy

Pending Publication Date: 2022-03-25
CHINA NAT INST OF STANDARDIZATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on the problems of large amount of samples and low efficiency in the detection of far-field terahertz spectroscopy, the present invention develops a sample cell suitable for near-field terahertz detection and provides a method for detecting antibody titer in the sample cell

Method used

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  • Sample cell for terahertz spectrum detection, method for evaluating antibody titer and application
  • Sample cell for terahertz spectrum detection, method for evaluating antibody titer and application
  • Sample cell for terahertz spectrum detection, method for evaluating antibody titer and application

Examples

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Embodiment 1

[0064] Example 1 (method for evaluating histone H3 antibody titer using the sample pool)

[0065] S1. Determine the detection object, the detection object includes: a) the antibody to be tested (histone H3 antibody, anti-histone H3) solution and the corresponding antigen (histone H3, histone H3) solution; b) the antibody to be tested solution-antigen mixed solution ;

[0066] S2, configure the detection object solution, as shown in Table 1, specifically:

[0067] Prepare 7 antigen solutions with a concentration of 20 μg / ml;

[0068] Dilute the antibody solution to be tested at 1, 2, 5, 10, 20, 50, 100 μg / ml from high concentration to low concentration;

[0069] And antibody solution-antigen mixed solutions with different binding ratios;

[0070] Table 1

[0071] serial number Antigen (μg / mL) Antibody (μg / mL) Antigen-antibody binding ratio 1 20 1 20:1 2 / 2 10:1 3 / 5 4:1 4 / 10 2:1 5 / 20 1:1 6 / 50 1:2.5 7 / ...

Embodiment 2

[0098] As one of the application examples of the present invention for evaluating antibody titer, this embodiment uses the method provided by the present invention to detect the titer of an antibody that binds to a certain antigen. By detecting different brands of antibodies that bind to the same antigen, the titers of different brands of antibodies can be qualitatively analyzed. Specifically:

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Abstract

The invention discloses a sample cell for terahertz spectrum detection, a method for evaluating antibody titer and application. The sample cell is of an array through hole structure made of a material with high terahertz wave transmittance and high visible light transmittance; the method comprises the following steps: S1, determining a sample to be detected; s2, preparing solutions with different concentrations of a sample to be detected; s3, adding a sample; s4, performing terahertz near-field spectrum detection on the to-be-detected sample liquid drops in each through hole to obtain terahertz signals of all the to-be-detected samples; s5, collecting and processing a terahertz signal of the sample to be detected; and S6, analyzing terahertz spectrum characteristics of different solutions before and after mixing. The sample in the sample pool only needs a trace amount of solution and is high in throughput, so that the detection cost is greatly reduced, and the detection efficiency and stability are improved; the sample solution is suspended in the sample cell by using siphonage, the collected spectrum only contains the sample, and the computational analysis model is simple and reliable. The method disclosed by the invention is small in dosage and high in efficiency; the method is free of marks and loss, and is higher in detection accuracy.

Description

technical field [0001] The invention belongs to the field of terahertz wave technology, and relates to a high-throughput detection device for trace liquid samples and a method for conformation analysis before and after antigen-antibody binding based on terahertz technology, specifically a sample pool for terahertz spectrum detection , Method and application for evaluating antibody titer. Background technique [0002] THz waves (terahertz waves) include electromagnetic waves with a frequency of 0.1 to 10 THz. The term applies to frequencies between the high-frequency edge (300GHz) of the millimeter-wave band of electromagnetic radiation and the low-frequency far-infrared spectral band edge (3000GHz), corresponding to wavelengths of radiation in this frequency band ranging from 0.03mm to 3mm ( Or 30-3000μm), the energy of the photon is about 1-10meV, which is roughly equivalent to the energy of the transition between molecular vibration and rotational energy levels. Most pol...

Claims

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Application Information

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IPC IPC(8): G01N21/3586G01N21/01
CPCG01N21/3586G01N21/01G01N2021/0112
Inventor 赵琳颜识涵云振宇吴琦吴希
Owner CHINA NAT INST OF STANDARDIZATION