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Application of benzbromarone in resisting infection of staphylococcus aureus and biofilm thereof

A technology of benzbromarone and staphylococcus, applied in the field of medicine, can solve problems such as prolonged infection duration, unsatisfactory biofilm effect, and failure of infection treatment

Pending Publication Date: 2022-04-01
华中科技大学协和深圳医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, Staphylococcus aureus can also form a biofilm (a bacterial colony formed by the bacteria adhering to the contact surface, secreting a variety of extracellular matrix, etc., and surrounding itself) to help the bacteria resist antibiotics, and the formation of a biofilm will lead to Prolonged infection duration is one of the important factors for the failure of infection treatment. However, the first-line antibacterial drugs currently used in clinical practice are not effective on biofilms, leading to an increased risk of death in patients related to this type of infection

Method used

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  • Application of benzbromarone in resisting infection of staphylococcus aureus and biofilm thereof
  • Application of benzbromarone in resisting infection of staphylococcus aureus and biofilm thereof
  • Application of benzbromarone in resisting infection of staphylococcus aureus and biofilm thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Minimum inhibitory concentration (Minimum inhibitory concentration, MIC) detection:

[0036]The minimum inhibitory concentration (MIC) was carried out by the micro broth dilution method, with ATCC29213 as the quality control strain, and the operation was carried out according to the CLSI-M100-S27 version. At the same time, vancomycin, linezolid, ampicillin and benzbromarone are used as comparative examples, and the results are shown in Table 1 below. MSSA and methicillin-resistant strain MRSA) have antibacterial and bactericidal effects, and their MIC is 16mg / L.

[0037] Table 1 The minimum inhibitory concentration of benzbromarone to Staphylococcus aureus

[0038]

Embodiment 2

[0040] The present embodiment is the experiment that the benzbromarone of detection sub-inhibitory concentration inhibits Staphylococcus aureus from forming biofilm, and specific method comprises:

[0041] Staphylococcus aureus methicillin-sensitive strains (MSSA) CHS100, CHS151, CHS160 and CHS46 and methicillin-resistant strains (MRSA) CHS823, CHS733, CHS736 and CHS727 strains were shaken in TSB medium at 37°C and 220rpm / min Cultivate overnight for 10-12h. Use TSBG medium (TSB medium + 0.5% glucose) to dilute the bacterial solution 1:200 (with or without therapeutic bacteria drugs), add 200ul per well to a 96-well plate (Costar3599), set 3 duplicate wells for each strain, and 37 Cultivate statically for 24 hours. Discard the supernatant, wash with PBS 3 times (200ul / well / time), dry at room temperature, add methanol for fixation for 15min (200ul / well), discard methanol and add 100ul of 0.5% crystal violet staining solution to each well after drying at room temperature. Stain...

Embodiment 3

[0044] This example verifies the experiment that benzbromarone effectively removes the biofilm formed by Staphylococcus aureus.

[0045] Staphylococcus aureus methicillin-sensitive strains (MSSA) CHS100, CHS151, CHS160 and CHS46 and methicillin-resistant strains (MRSA) CHS823, CHS733, CHS736 and CHS727 were shaken in TSB medium at 37°C and 220rpm / min overnight Cultivate for 10-12h. Dilute the bacterial solution 1:200 with TSBG medium (TSB medium + 0.5% glucose), add 200ul per well to a 96-well plate (Costar 3599), set 3 duplicate wells for each strain, and culture at 37°C for 24 hours to form a mature biofilm. Discard the supernatant, wash with sterile normal saline for 3 times (200ul / well / time), then add fresh TSBG medium (containing different concentrations of benzbromarone), and culture for 48h (replace with a new one every 24h). After that, discard the supernatant, wash with PBS 3 times (200ul / well / time), dry at room temperature, add methanol for fixation for 15min (200u...

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Abstract

The invention provides an application of benzbromarone in resisting staphylococcus aureus and biofilm infection thereof. The benzbromarone is used for resisting staphylococcus aureus and biofilm infection thereof. The technical scheme of the invention discloses a new application of benzbromarone. The benzbromarone has bacteriostasis and sterilization capabilities on staphylococcus aureus and biofilm thereof; the benzbromarone under the sub-inhibitory concentration can obviously inhibit the growth of staphylococcus aureus and the formation of a biofilm; a biofilm formed by the staphylococcus aureus can be removed when the minimum inhibitory concentration is greater than or equal to two times, the staphylococcus aureus in the biofilm can be effectively killed, and the hemolytic activity of the staphylococcus aureus on red blood cells is inhibited by the sub-inhibitory concentration.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to the use of benzbromarone for resisting Staphylococcus aureus and its biofilm infection. Background technique [0002] Staphylococcus aureus, especially Methicillin-Resistant Staphylococcus aureus (MRSA) has become a major cause of hospital and community-acquired infections worldwide, causing endocarditis, osteomyelitis, necrosis, etc. Severe and fatal infections such as acute pneumonia and sepsis. Because MRSA is resistant to multiple antibacterial drugs, vancomycin and linezolid are often used clinically as effective drugs for the treatment of severe MRSA infections, although daptomycin and dalbavancin, which are effective against MRSA, have also been used clinically in recent years However, with the continuous mutation of MRSA and the acquisition of new drug-resistant genes, drug-resistant strains of these drugs appeared shortly after clinical application. With the increasin...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61P31/04
Inventor 尚永朋邓启文余治健孟清音
Owner 华中科技大学协和深圳医院
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