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Application of tetrahedral framework nucleic acid in medicine for treating pancreatitis

A tetrahedral, pancreatitis technology, which is applied to the tetrahedral framework nucleic acid in the field of drugs for the treatment of pancreatitis, can solve the problems of poor editability, multi-organ damage, large side effects, etc., and achieves the effect of reducing multi-organ damage and good application prospects.

Active Publication Date: 2022-04-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, existing treatment methods are often difficult to treat multiple organ damage caused by SAP while anti-inflammation, and some drugs such as antibiotics often have side effects Larger, poor editability, if drug resistance occurs, it is difficult to further adjust and upgrade

Method used

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  • Application of tetrahedral framework nucleic acid in medicine for treating pancreatitis
  • Application of tetrahedral framework nucleic acid in medicine for treating pancreatitis
  • Application of tetrahedral framework nucleic acid in medicine for treating pancreatitis

Examples

Experimental program
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Embodiment 1

[0029] Embodiment 1, the synthesis of tetrahedral framework nucleic acid

[0030] Dissolve four DNA single strands (S1, S2, S3, S4) in TM Buffer (10mM Tris-HCl, 50mM MgCl 2 ,pH=8.0), the final concentration of the four DNA single strands was 1 μM, mixed thoroughly, rapidly heated to 95°C for 10 minutes, and then rapidly cooled to 4°C and maintained for more than 20 minutes to obtain tetrahedral framework nucleic acid tFNAs.

[0031] The sequences of the four single strands are as follows:

[0032]

[0033] Synthesized tetrahedral framework nucleic acid, PAGE gel electrophoresis ( figure 1 ) and capillary electrophoresis ( figure 2 ) It can be seen that the size of the tetrahedral framework nucleic acid is about 180KD, and it can be considered that the tetrahedral framework nucleic acid has been successfully synthesized. Tetrahedral particle size is about 20.12nm ( image 3 ), the potential is -6.29mV ( Figure 4 ). Using a transmission electron microscope, the tetrah...

experiment example 1

[0035] Experimental example 1. Therapeutic effect of tetrahedral framework nucleic acid on acute pancreatitis model

[0036] 1. Experimental method

[0037] (1) C57BL / 6 mice (male, 6-8 weeks old, 22±2g) were purchased, fed with standard feed and sterile water, and fed under laboratory conditions for 1 week for adaptive feeding.

[0038] (2) Before the start of the experiment, the mice were fasted for 12 hours. In this paper, 2% sodium taurocholate (US Sigma) was injected into the bile-pancreatic duct to induce SAP using a micropump.

[0039] (3) 21 mice were randomly divided into saline (Saline) group, tFNAs group and sham operation (SO) group. In the tFNAs-treated group, mice were injected with tFNAs (125nM, 100ul) twice through the tail vein at 30 minutes and 60 minutes after SAP induction, respectively. At the same time, 30 and 60 minutes after SAP induction, the mice in the normal saline group were also given the same amount of normal saline solution each time. Mice in...

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Abstract

The invention provides application of tetrahedral framework nucleic acid in medicine for treating pancreatitis. The tetrahedral framework nucleic acid is formed by four single-stranded DNAs through base complementary pairing; the sequences of the four single-stranded DNAs are respectively selected from the sequences as shown in SEQ ID NO.1-4 in a one-to-one manner. The tetrahedral framework nucleic acid shows excellent anti-inflammatory and anti-apoptosis capabilities in treatment of severe acute pancreatitis, reduces new severe acute pancreatitis and multi-organ injury caused by the new severe acute pancreatitis, is a safe and effective clinical choice, and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the use of tetrahedral framework nucleic acid in medicine for treating pancreatitis. Background technique [0002] Acute pancreatitis (AP) is an inflammatory reaction in which pancreatic tissue is digested, edema, hemorrhage or even necrosis after the activation of pancreatic enzymes. It is one of the most common inflammatory diseases. It is characterized by fever and increased blood pancreatic enzymes, and the mortality rate of confirmed patients exceeds 5%. AP is mostly divided into mild and severe. Mild acute pancreatitis is more common clinically. Pancreatic edema is the main symptom. The condition is often self-limited and the prognosis is good. About 20% of AP will develop into severe acute pancreatitis (SAP) within a short period of time, accompanied by pancreatic tissue damage and necrosis. To make matters worse, SAP eventually leads to multiple organ failure in t...

Claims

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Application Information

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IPC IPC(8): A61K31/713A61P1/18A61P1/16A61P13/12A61P11/00A61P7/04A61P7/10
Inventor 林云锋王韵高邵静雅蔡潇潇
Owner SICHUAN UNIV
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