Bacillus subtilis and application thereof
A technology of Bacillus subtilis and strains, applied in the field of Bacillus subtilis and its application, can solve the problems of large amount of sludge, failure to meet discharge standards, high operating costs, etc.
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experiment example 1
[0041] Screening of Bacillus subtilis SWS3
[0042] Sampling was taken from the aerobic pool of papermaking wastewater and domestic garbage sewage treatment aerobic pool, and after separation and purification, two strains of Bacillus subtilis were obtained, named ZZ-1 and LJ-5 respectively, wherein LJ-5 is the subtilis provided by the present invention Bacillus SWS3 was deposited in the China Center for Type Culture Collection (CCTCC) on November 1, 2021, with the deposit number CCTCC M20211349.
[0043] See the results of Gram staining for Bacillus subtilis SWS3 figure 1 , identified as positive bacillus. Green oval spores can be seen in the field of spore staining figure 2 , cultivated in LB solid medium as round yellow-white colonies, opaque, dull, and the surface of the colonies is not smooth. image 3 .
[0044] Bacillus subtilis ZZ-1, LJ-5 in NO 2 Media, NO 3 Media and NH 4 Cultivate in culture medium, observe the growth situation of Bacillus subtilis ZZ-1, LJ-5,...
Embodiment 1
[0049] The sample adopts the low-temperature vacuum drying condensate of kitchen waste provided by Weihai Wendeng.
[0050] After the kitchen waste is dried in a low-temperature vacuum, volatile substances with a lower boiling point will pass through the condenser to form a condensate, and the condensate will be analyzed.
[0051] The above-mentioned condensate was used as a control sample by using the automatic headspace pretreatment method, and its volatile matter components were analyzed by gas chromatograph. The main components are shown in Table 1.
[0052] The COD of the control sample was determined by the dichromate method to be 2283 mg·L -1 .
[0053] Image 6 is the gas chromatogram of the control sample.
[0054] Table 1
[0055]
Embodiment 2
[0057] Treatment of the control sample in Example 1
[0058] Pick a single colony of Bacillus subtilis SWS3 and inoculate it into LB liquid medium, and cultivate to OD at 37°C and 150rpm. 600 is 0.6, the seed liquid is prepared, and the seed liquid is transferred to the control sample in Example 1 with the inoculation amount of 5mL / 100mL, and the biological nitrogen and inorganic salt components that satisfy the growth of the bacteria are added, and degraded at 150rpm and 37°C. After 48 hours of treatment, experimental samples treated with Bacillus subtilis SWS3 were obtained. After the experimental samples were centrifuged at 5000 rpm to remove the bacteria, the liquid was retained, and the automatic headspace pretreatment method was used to analyze the volatile substances in the retained liquid by gas chromatography. The main components are shown in Table 2.
[0059] The above-mentioned biological nitrogen is urea, and the final concentration is 0.2%; the inorganic salt com...
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