Bacillus subtilis and application thereof

A technology of Bacillus subtilis and strains, applied in the field of Bacillus subtilis and its application, can solve the problems of large amount of sludge, failure to meet discharge standards, high operating costs, etc.

Pending Publication Date: 2022-04-08
BIOLOGY INST OF SHANDONG ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent document CN201210129450 (application patent number CN103145292A) discloses a leachate treatment process and device in a small-scale garbage transfer station. The patent uses two treatment processes, micro-electrolysis and MBR, so that the COD of the leachate after treatment is 1000mg / L within, but still not up to emission standards
[0004] Chinese patent document CN113087275A (patent application number: CN202110525842.9) discloses a pretreatment system for landfill leachate. This patent can effectively remove pollutants such as suspended solids, heavy metals and ammonia nitrogen in landfill leachate, but cannot effectively Removal of remaining components in landfill leachate
[0005] Chinese patent document CN210711136U (patent application number 201920659099.4) proposes an intelligent integrated equipment for waste water treatment in a garbage transfer station. The patent design leachate passes through the coagulation reactor, anaerobic digestion tank, primary contact oxidation tank and secondary contact oxidation in sequence. Pools and MBR membrane bioreactors can obtain high-quality effluent, but the overall design process is too large, and the amount of sludge generated is mostly
[0006] Chinese patent document CN111646599A (patent application number CN202010560761.8) proposes a system for integrated treatment of leachate in a garbage transfer station. This patent only allows the leachate that has undergone coagulation and sedimentation to directly enter the NF and RO membrane treatment devices, although COD The removal efficiency reaches 99.6%, but the frequent replacement of the membrane causes the operation cost to be too high
[0007] The above-mentioned patent documents disclose a variety of treatment methods for landfill leachate, but the treatment process is complicated and the operating cost is too high; therefore, there is an urgent need for a microbial strain that can efficiently degrade volatile organic compounds (VOCs) in the treatment of waste liquid and waste gas to simplify the treatment. process, reducing operating costs for processing
[0008] Although there are many types of biological deodorants for waste gas treatment in the prior art, the disclosed biological deodorants are composed of a variety of microorganisms; Composite bacterial agent for waste gas, which is composed of Pseudomonas putida, Bacillus subtilis, Bacillus cereus, Thiobacillus thiooxidans, Lactobacillus plantarum, and Thiobacillus thiooxidans; high

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0041] Screening of Bacillus subtilis SWS3

[0042] Sampling was taken from the aerobic pool of papermaking wastewater and domestic garbage sewage treatment aerobic pool, and after separation and purification, two strains of Bacillus subtilis were obtained, named ZZ-1 and LJ-5 respectively, wherein LJ-5 is the subtilis provided by the present invention Bacillus SWS3 was deposited in the China Center for Type Culture Collection (CCTCC) on November 1, 2021, with the deposit number CCTCC M20211349.

[0043] See the results of Gram staining for Bacillus subtilis SWS3 figure 1 , identified as positive bacillus. Green oval spores can be seen in the field of spore staining figure 2 , cultivated in LB solid medium as round yellow-white colonies, opaque, dull, and the surface of the colonies is not smooth. image 3 .

[0044] Bacillus subtilis ZZ-1, LJ-5 in NO 2 Media, NO 3 Media and NH 4 Cultivate in culture medium, observe the growth situation of Bacillus subtilis ZZ-1, LJ-5,...

Embodiment 1

[0049] The sample adopts the low-temperature vacuum drying condensate of kitchen waste provided by Weihai Wendeng.

[0050] After the kitchen waste is dried in a low-temperature vacuum, volatile substances with a lower boiling point will pass through the condenser to form a condensate, and the condensate will be analyzed.

[0051] The above-mentioned condensate was used as a control sample by using the automatic headspace pretreatment method, and its volatile matter components were analyzed by gas chromatograph. The main components are shown in Table 1.

[0052] The COD of the control sample was determined by the dichromate method to be 2283 mg·L -1 .

[0053] Image 6 is the gas chromatogram of the control sample.

[0054] Table 1

[0055]

Embodiment 2

[0057] Treatment of the control sample in Example 1

[0058] Pick a single colony of Bacillus subtilis SWS3 and inoculate it into LB liquid medium, and cultivate to OD at 37°C and 150rpm. 600 is 0.6, the seed liquid is prepared, and the seed liquid is transferred to the control sample in Example 1 with the inoculation amount of 5mL / 100mL, and the biological nitrogen and inorganic salt components that satisfy the growth of the bacteria are added, and degraded at 150rpm and 37°C. After 48 hours of treatment, experimental samples treated with Bacillus subtilis SWS3 were obtained. After the experimental samples were centrifuged at 5000 rpm to remove the bacteria, the liquid was retained, and the automatic headspace pretreatment method was used to analyze the volatile substances in the retained liquid by gas chromatography. The main components are shown in Table 2.

[0059] The above-mentioned biological nitrogen is urea, and the final concentration is 0.2%; the inorganic salt com...

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Abstract

The invention provides a strain of bacillus subtilis SWS3, which is preserved in China Center for Type Culture Collection on November 1, 2021, the preservation address is Wuhan University, and the preservation number is CCTCC M 20211349; the strain can be used for effectively degrading methyl mercaptan, 2-methyl episulfide ethane, butyric acid, valeric acid and eudesmol; the strain can degrade volatile substances in the kitchen waste low-temperature vacuum drying condensate within 48 hours, and the COD (Chemical Oxygen Demand) of the condensate is reduced from 2283 mg.L <-1 > to 134 mg.L <-1 >, so that the condensate directly meets the wastewater discharge standard.

Description

technical field [0001] The invention relates to a bacillus subtilis and application thereof, belonging to the field of waste water treatment. Background technique [0002] With the continuous development of my country's catering industry and the improvement of people's living standards, the discharge of kitchen waste is increasing day by day, and kitchen waste contains extremely high moisture and organic matter, which is easy to rot and produce stench. During the treatment process, in order to reduce the adverse effects of excessive moisture on the post-processing of garbage and its corrosion on equipment, it can be dried in a low-temperature vacuum first to reduce moisture and facilitate the next step of treatment. However, some volatile organic compounds (VOCs) will be produced during the low-temperature vacuum drying process. VOCs are important precursors for the formation of secondary pollutants such as fine particulate matter (PM2.5) and ozone (O3), which in turn cause h...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B01D53/84C12R1/125
CPCY02A50/20
Inventor 楚杰蔡春静张亦桑卢俊桦王莹宋文著
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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