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Poly L-phenylalanine/nanogold/shewanella MR-1/multi-walled carbon nanotube modified electrode, preparation method and application

A technology of multi-walled carbon nanotubes and Shewanella, applied in the direction of electrochemical variables of materials, etc., can solve the problems of expensive equipment, high detection cost, complicated measurement operations, etc., and achieves fast detection speed, high detection sensitivity, and responsiveness. strong signal effect

Pending Publication Date: 2022-04-12
ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The determination operation is complicated, the required equipment is expensive, and the detection cost is high

Method used

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  • Poly L-phenylalanine/nanogold/shewanella MR-1/multi-walled carbon nanotube modified electrode, preparation method and application
  • Poly L-phenylalanine/nanogold/shewanella MR-1/multi-walled carbon nanotube modified electrode, preparation method and application
  • Poly L-phenylalanine/nanogold/shewanella MR-1/multi-walled carbon nanotube modified electrode, preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0053] S1. Preparation of Bare Electrode (GCE)

[0054] First place the glassy carbon electrode with 0.05 μm Al 2 o 3 Powdered suede is sanded to a mirror finish, then rinsed with distilled water. Place them into distilled water, absolute ethanol and distilled water for 3 minutes for ultrasonication, take them out, rinse them with distilled water, and dry them naturally to obtain a bare electrode (GCE).

[0055] S2. Preparation of Shewanella MR-1 / multi-walled carbon nanotube composite solution

[0056] Weigh 1 mg of multi-walled carbon nanotubes and dilute with 1 mL of sterile aqueous solution, add 0.03 mg of Shewanella MR-1, and mix thoroughly by ultrasonication at 30 °C;

[0057] S3. Preparation of Shewanella MR-1 / multi-walled carbon nanotube modified electrode (MR-1 / MWCNT / GCE)

[0058] Pipette 5.0 μL of Shewanella MR-1 / multi-walled carbon nanotube composite material solution and evenly drop it on the surface of the bare electrode prepared in S1, and dry it to obtain She...

Embodiment 2

[0080] In the scanning voltage range of -0.45 ~ 0.6V, the scanning speed is 80 ~ 120mV / s, and the electrochemical behavior of baicalein solution on the modified electrode is determined by cyclic voltammetry scanning. At room temperature, differential pulse voltammetry is used for different concentrations Quantitative analysis of baicalein solutions. The baicalein solution is prepared from a 0.1mol / L, pH=6.3 phosphate buffer solution.

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Abstract

The invention provides a poly (L-phenylalanine) / nanogold / shewanella MR-1 / multi-walled carbon nanotube modified electrode, a preparation method and application, and belongs to the technical field of glassy carbon electrodes, and the preparation method comprises the following steps: polishing the surface of a glassy carbon electrode, cleaning, dropwise adding a shewanella MR-1 / multi-walled carbon nanotube composite material solution on the surface, drying, taking the electrode as a working electrode, and carrying out vacuum drying on the working electrode to obtain the poly (L-phenylalanine) / nanogold / shewanella MR-1 / multi-walled carbon nanotube modified electrode. The preparation method comprises the following steps: taking a shewanella MR-1 / multi-walled carbon nanotube modified electrode as a substrate, putting the shewanella MR-1 / multi-walled carbon nanotube modified electrode into a chloroauric acid aqueous solution, carrying out potentiostatic deposition of nanogold by adopting a chronoamperometry, and carrying out cyclic voltammetry scanning, washing and drying in an L-phenylalanine solution by taking the electrode as a working electrode to obtain the poly L-phenylalanine / nanogold / shewanella MR-1 / multi-walled carbon nanotube modified electrode. The electrode prepared by the invention has the characteristics of good catalytic action on baicalein, high sensitivity, high detection speed and the like. The electrode is combined with cyclic voltammetry scanning and differential pulse voltammetry to quantitatively detect baicalein, and has the advantages of high sensitivity, high response speed, strong response signal and the like.

Description

technical field [0001] The invention relates to the technical field of glassy carbon electrodes, in particular to a poly-L-phenylalanine / nano gold / Shewanella MR-1 / multi-walled carbon nanotube modified electrode, a preparation method and an application. Background technique [0002] Baicalein (BAI) is a commonly used traditional Chinese medicine, which exists in some plants such as the leaves of Scutellaria baicalensis and the leaves and roots of Scutellaria baicalensis. It has the effects of clearing away heat and dampness, purging fire and detoxifying. Its molecular structure belongs to flavonoids. It has been found that BAI has many special functions, including anti-oxidation and free radical scavenging effects, fatty liver prevention, anti-tumor, anti-allergic and anti-inflammatory effects, so it is very important for the research of BAI. BAI detection means mainly through capillary electrophoresis, high performance liquid chromatography (HPLC), combined technology, sta...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
Inventor 陶亦文许春萱
Owner ZHENGZHOU UNIV
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