Method for storing and releasing protein-modified nanoparticles on paper substrate

A protein modification and nanoparticle technology, applied in nanotechnology, chemical instruments and methods, nanotechnology, etc. for materials and surface science, can solve problems such as low efficiency and insufficient characterization of nanoparticle release, and achieve easy manufacturing Effect

Pending Publication Date: 2022-04-12
UNIV DE LAS ISLAS BALEARES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The release of nanoparticles stored in unmodified paper substrates has not been fully characterized (Chen et al. Three-dimensional origami paper-based device for portable immunoassay applications. Lab Chip 2019, 19, 598–607), and has been reported in some cases The efficiency is extremely low (Ruivo et al., 2017)

Method used

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  • Method for storing and releasing protein-modified nanoparticles on paper substrate
  • Method for storing and releasing protein-modified nanoparticles on paper substrate
  • Method for storing and releasing protein-modified nanoparticles on paper substrate

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Embodiment Construction

[0055] Synthesis and modification of gold nanoparticles with polyethylene glycol and avidin: Citrate-terminated gold nanoparticles with a diameter of approximately 40 nm were synthesized using the Turkevich method as previously described (Russell et al., Augmented Reality for Real-Time Detection and Interpretation of Colorimetric Signals Generated by Paper-Based Biosensors. ACS Sensors 2017, 2, 848–853). The nanoparticles were then modified overnight with 0.1 mM thiolated polyethylene glycol (PEG) molecules terminated with carboxylate moieties (poly(ethylene glycol) 2-mercaptoethyl ether acetic acid, Mn2100, Sigma). The resulting PEGylated nanoparticles were concentrated and washed 5 times with water by centrifugation at 8000 rpm for 6 minutes. Finally, the nanoparticles were suspended in 0.5M 2-(N-morpholino)ethanesulfonic acid (MES, Sigma) with pH adjusted to 5.5. Then by adding 1 mg of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC, Sigma) and 2 mg of N-...

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Abstract

The present invention relates to a method of storing protein-modified nanoparticles on a paper substrate without irreversible binding, in particular a method of storing protein-modified nanoparticles within a polymer-modified paper substrate. The invention also relates to a reservoir comprising protein-modified nanoparticles capable of establishing biological interactions obtained by this method and to the use thereof in biosensors made of paper.

Description

[0001] The present invention relates to a method for storing protein-modified nanoparticles on a paper substrate without irreversible binding, in particular a method for storing protein-modified nanoparticles in a polymer-modified paper substrate. The invention also relates to reservoirs comprising protein-modified nanoparticles capable of establishing biological interactions obtained by this method and their use in biosensors made of paper. Background technique [0002] Filter paper is the substrate of choice for developing disposable biosensors because of its light weight, low price and easy disposal (Reboud et al. Paper-based microfluidics for DNA diagnostics of malaria in lowresource underserved rural communities. Proc. Natl. Acad. Sci. USA 2019, 116, 4834–4842; Roig-Sanchez et al. Nanocellular films with multiple functional nanoparticles in confined spatial distribution Nanoscale Horiz., 2019, 4, 634--641; Tenda et al., Paper-Based Antibody Detection Devices Using ET Biolu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00B82Y5/00B82Y30/00D21H17/35D21H19/22G01N33/531G01N33/543
CPCG01N33/54306G01N33/54393G01N33/54346G01N33/544G01N33/548G01N33/52B01L3/00G01N33/566G01N33/531D21H17/35D21H19/22B82Y5/00B82Y30/00B01L2300/126
Inventor R·德·拉·里卡·克萨达F·A·阿尔巴·帕蒂诺C·阿卓瓦·贾米
Owner UNIV DE LAS ISLAS BALEARES
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