Good pholiota nameko strain as well as specific molecular marker and application thereof

A specific technology of the mushroom, which is applied in the field of microorganisms, can solve the problems of strain aging and failure to be properly resolved, and achieve the effects of short harvesting period, strong anti-pathogen ability, and high biotransformation rate

Pending Publication Date: 2022-04-15
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the mushroom is easy to cultivate, the aging problem of its strains has not been prop...

Method used

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  • Good pholiota nameko strain as well as specific molecular marker and application thereof
  • Good pholiota nameko strain as well as specific molecular marker and application thereof
  • Good pholiota nameko strain as well as specific molecular marker and application thereof

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Experimental program
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Effect test

Embodiment 1

[0045] Identification of embodiment 1 parent bacterial strain

[0046] The fruiting bodies of the wild Pleurotus chinensis were collected from Medog, Tibet, and the strain was proved to be Pyrethia spp. through morphological observation and nuclear rDNA-ITS sequencing (SEQ ID NO: 3). The fruiting bodies of wild Pleurotus chinensis were isolated, purified and cultured strain 682 was compared and screened for cultivation.

[0047] The specific selection method is as follows:

[0048] Cultivation of wild strains: Cultivate wild isolated strains, use PDA medium for mother species cultivation, wheat grain medium for original species cultivation, and sawdust compost for cultivated species. After the hyphae are covered with the culture material, the bag is opened and the mushroom is covered with soil. Strain 682 produced neat mushrooms with basically the same maturity.

[0049] The cultivation method of Pleurotus 682 comprises the following steps:

[0050] (1) Propagation of the ...

Embodiment 2

[0060] Example 2 Pleurotus chinensis strain comparison experiment

[0061] 1. Comparison test of the strains of Pleurotus ostreatus

[0062] The strain 682 was cultivated with the commercially available strains C3 and 112 of Pleurotus chinensis. The parent species was cultivated using PDA medium, the original species was cultivated using wheat grain medium, and the cultivars were cultivated using sawdust compost. After the hyphae are covered with sawdust compost and the color changes for one month, the bag is opened and covered with soil to produce mushrooms. Compare its fruiting situation and output.

[0063] The results are shown in Table 1. Compared with the commercially available strains, the strain 682 has denser mushroom buds, faster fruiting, and a shorter harvest cycle, only 26±1d from covering soil to harvesting; the biotransformation rate is higher, and the biotransformation rate reaches 112±0.9 %.

[0064] Table 1 The results of comparison between strain 682 and ...

Embodiment 3

[0072] The specific DNA molecular marker of embodiment 3 Huazi mushroom 682

[0073] A large number of screening tests were carried out using PCR technology. Among them, using the primers shown in SEQ ID NO: 1 and 2 (6063-1027F: 5'-AAATGGTAACGTCGAGTGCG-3' and 6063-1027R: 5'-CGTTTGACGTGATCGCAATGTT-3') to obtain the specific DNA fragment of the mushroom species 682 , the size is about 1027bp (SEQ ID NO: 4), and the same gene fragment is different from other Pleurotus spp. strains (SEQ ID NO: 4), which is the specific molecular marker of Pleurotus spp. strain 682.

[0074] PCR reaction system (20μl): Taq-Mix 10μl, upstream and downstream primers 0.5μl, template DNA 2μl, ddH 2 O 7 μl.

[0075] PCR reaction program: 95°C for 5min; 35 cycles of 95°C for 30s, 60°C for 30s, 72°C for 15s; 72°C for 10min.

[0076] The electrophoresis pattern of the specific DNA fragments of the 682 strain of P. Figure 7 , in which C3 is the strain C3 of P. chinensis (commercially available strain),...

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Abstract

The invention discloses a good pholiota nameko strain as well as a specific molecular marker and application thereof. The excellent strain is pholiota nameko 682 (the preservation number is CGMCCNO: 23243) which is high in yield, fast in fruiting and strong in disease resistance. The pholiota nameko 682 mycelia grow fast, the after-ripening period is short, fruiting is fast, mushroom buds are dense, the survival rate of the mushroom buds is 80% or above, pilei are light yellow to yellowish brown, the adaptive capacity is high, and the harvesting period is short; the biological efficiency is high; the strain has strong pathogenic bacterium resistance, and has strong antagonistic ability to trichoderma harzianum, penicillium expansum and fusarium. The pholiota nameko strain 682 is obtained through wild specimen separation, a new edible mushroom variety is developed, the specific DNA sequence and the specific enzyme cutting site of the pholiota nameko 682 are obtained, and the method has important significance in protecting germplasm resources of the species, enriching edible mushroom variety resources and the like.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an excellent bacterial strain of Pleurotus chinensis and its specific molecular marker and application. Background technique [0002] Pholiota microspora belongs to the kingdom Fungi, phylum Basidiomycota, class Agaricaceae, order Agaricaceae, family Strophariaceae, and genus Agarita. Also known as slippery mushroom, pearl mushroom, pearl mushroom, slider mushroom, light cap scale umbrella. Slide mushroom has extremely high edible and nutritional value, which is rich in nutrients, such as crude protein, crude fiber, polysaccharides, fat, minerals, ash, vitamins, etc., and has anti-tumor, anti-oxidation and immune-enhancing effects . Huazi mushroom is one of the top ten mushrooms in the world's mushroom trading market, and it is also one of the edible fungi species recommended by the Food and Agriculture Organization of the United Nations (FAO) to developing countri...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/11C12Q1/6895C12Q1/686C12Q1/04A01N63/30A01P3/00A01G18/00A01G18/20C12R1/645
Inventor 董彩虹徐岩岩
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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