Yeast exopolysaccharide, preparation method and application thereof

An extracellular polysaccharide and yeast technology, applied in the field of microorganisms, can solve the problems of high production cost, low yield and the like, achieve the effect of simple and efficient preparation method, and protect Lactococcus lactis from freeze-drying damage

Active Publication Date: 2022-04-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, microbial exopolysaccharides have been favored by researchers due to their unique physical and chemical properties, biological safety and biological activity, but low yield and high production cost have always been the main factors restricting their large-scale production and application.

Method used

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  • Yeast exopolysaccharide, preparation method and application thereof
  • Yeast exopolysaccharide, preparation method and application thereof
  • Yeast exopolysaccharide, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1. Preparation of exopolysaccharide of Saccharomyces ruxii of the present invention

[0050] (1) Activate and expand the culture of Zygosaccharomyces rouxii CGMCC No.3791;

[0051] (2) Inoculate the expansion culture solution prepared in step (1) into the fermentation medium with an inoculum size of 5%, and culture it statically at 30° C. for 60 hours to obtain a fermentation solution containing exopolysaccharide of Saccharomyces ruxii;

[0052] The fermentation medium consists of the following components: yeast extract 1g, peptone 2g, glucose 2g, distilled water 100mL;

[0053] (3) centrifuging the liquid fermentation broth prepared in step (2), and collecting the supernatant;

[0054] (4) adding trichloroacetic acid with a final concentration of 3-8% to the supernatant obtained in step (3), stirring at room temperature for half an hour, centrifuging, and collecting the supernatant;

[0055] (5) adding three times the volume of absolute ethanol to the supe...

Embodiment 2

[0062] Embodiment 2. Identification of exopolysaccharide of Saccharomyces ruxii of the present invention

[0063] 2.1 Molecular weight determination

[0064] Specific steps are as follows:

[0065] (1) Dissolve the EPS-3791 obtained in Example 1 in 0.1M NaNO 3 In aqueous solution to a final concentration of 1 mg / mL, and filtered through a filter with a pore size of 0.45 μm;

[0066] (2) Inject the filtrate (100 μL) prepared in step (1) into a gel exclusion chromatography column (Ohpak SB-805HQ (300×8mm), Ohpak SB-804HQ (300×8mm), Ohpak SB-803HQ (300×8mm), 8mm)), eluted with sodium nitrate solution (0.1M) at a flow rate of 0.4mL / min, and the column temperature was 45°C to obtain the response value data;

[0067] (3) Collect and process the obtained data, and calculate the molecular weight according to the Mark-Houwink Equation.

[0068] The measurement result is: the average molecular weight of EPS-3791 is 64.412KDa.

[0069] 2.2 Determination of the composition of monosac...

Embodiment 3

[0084] Embodiment 3. The function of exopolysaccharide of Saccharomyces rouxii of the present invention

[0085] 3.1 Low-temperature protective effect of the exopolysaccharide EPS-3791 of Saccharomyces ruckeri of the present invention on Lactococcus lactis

[0086] Specific steps are as follows:

[0087] (1) Take 1 mL of the mid-log phase fermentation broth of Lactococcus lactis MG 1363, centrifuge (8 000 g, 4°C, 5 min), and discard the supernatant;

[0088] (2) Add 1 mL of 0.9% normal saline (group without any protective agent), 20% glycerin, 1% EPS, 3% EPS, 5% EPS aqueous solution, shake well;

[0089] (3) Freeze at -80°C for 7 days, thaw, calculate the number of viable bacteria by the dilution coating plate method, and the survival rate is expressed as the percentage of the number of viable cells after freezing relative to the number of viable cells before freezing.

[0090] The measurement results are as follows: Figure 5 As shown in middle a, after 7 days of cryogenic...

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Abstract

The invention provides saccharomycetes exopolysaccharide as well as a preparation method and application thereof, and relates to the technical field of microorganisms. The saccharomycetes exopolysaccharide is produced by fermentation of saccharomycetes rouxii, the saccharomycetes rouxii is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the saccharomycetes is CGMCC (China General Microbiological Culture Collection Center) No.3791. The saccharomycetes exopolysaccharide is prepared by fermentation of saccharomycetes rouxii, and the saccharomycetes is preserved in the China General Microbiological Culture Collection Center. The saccharomycetes exopolysaccharide disclosed by the invention has low-temperature protection and freeze-drying protection effects on other strains, especially lactococcus lactis, and is suitable for popularization and application.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a yeast exopolysaccharide, a preparation method and an application thereof. Background technique [0002] Exopolysaccharide (EPS) is a secondary metabolite produced by microorganisms during growth and metabolism, some of which are attached to the cell wall to form capsular polysaccharides; some are secreted into the surrounding environment to form mucus polysaccharides. In recent years, microbial exopolysaccharides have been favored by researchers because of their unique physical and chemical properties, biological safety and biological activity, but low yield and high production cost have always been the main factors restricting their large-scale production and application. Because of its antioxidant, antibacterial, antiviral, antitumor and immunomodulatory activities, exopolysaccharides have broad application prospects in the healthy life of human beings. [0003] In ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12N1/16A61K8/73A61K31/715A61K36/064A61P31/04A61P31/10A61P31/12A61P35/00A61P37/02A61P39/06A61Q17/00A61Q19/00A61Q19/08C12R1/645
Inventor 吴重德张敏曾思源金垚黄钧周荣清
Owner SICHUAN UNIV
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