Application of herba lycopi flavone in preparation of medicine for resisting hyperuricemia and gout

A technology for hyperuricemia and uric acid, applied in the field of medicine, can solve the problems that the anti-hyperuricemia and/or anti-gout drug effects of Zelan flavonoids have not been reported yet.

Pending Publication Date: 2022-04-22
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the effect of Eupatorium flavonoids on anti-hyperuricemia and / or anti-gout drugs has not been reported

Method used

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  • Application of herba lycopi flavone in preparation of medicine for resisting hyperuricemia and gout
  • Application of herba lycopi flavone in preparation of medicine for resisting hyperuricemia and gout
  • Application of herba lycopi flavone in preparation of medicine for resisting hyperuricemia and gout

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0032] Experimental example 1. Eupatorium flavone reduces the activity of xanthine oxidase in vitro.

[0033] Experimental materials: Eupatorium flavonoids were obtained from the sample library of the Screening Center of the Institute of Materia Medica, Chinese Academy of Medical Sciences, allopurinol, tert-butylhydroquinone, xanthine oxidase, and EDTA were purchased from Sigma-Aldrich (Germany) . WST-1 was purchased from Dongren Chemical Technology (Shanghai) Co., Ltd.

[0034] Table 1. Reaction system:

[0035]

[0036] After adding the sample into the 384-well plate according to the reaction system in Table 1, monitor continuously for 20 minutes to detect the absorbance at 295nm and 450nm, and calculate the slope change of absorbance at 295nm. The slope of the 295nm absorption peak represents the rate of uric acid generation, which can be used to evaluate the activity of xanthine oxidase; OD495 represents the amount of formazan generated by WST-1, which can be used to ...

experiment example 2

[0042] Experimental example 2. Eupatorium flavone's effect of lowering uric acid in mice.

[0043] Experimental materials: c57BL / 6J mice were purchased from Beijing Huafukang Biotechnology Co., Ltd. Potassium oxonate, benzbromarone was purchased from Sigma-Aldrich, Germany. Eupatorium flavonoids were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Sodium carboxymethyl cellulose was purchased from Sinopharm Chemical Reagent Co., Ltd. The uric acid kit was purchased from Zhongsheng Beikong Biotechnology Co., Ltd.

[0044] Solution preparation: dissolve 0.5% sodium carboxymethyl cellulose, boil it, and use it as a solvent after cooling, and dissolve euphratica flavone, potassium oxonate, and benzbromarone respectively to form a suspension.

[0045] Experimental grouping: 18g mice were randomly divided into normal control group, model group, benzbromarone group (20mg·kg -1 , positive control), Eupatorium flavone low and high dose groups (5, 20mg·kg -1 ), 5-8 in every g...

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Abstract

The invention belongs to the technical field of medicines, and discloses application of herba lycopi flavone in preparation of anti-hyperuricemia and/or anti-gout medicines, hyperuricemia comprises primary and secondary hyperuricemia, and the concentration of uric acid in blood caused by various factors is higher than 420 [mu] mol/L for men and 357 [mu] mol/L for women (measured by a phosphotungstic acid reduction method). Or determining adult gout higher than 420 mu mol/L by a uricase-peroxidase coupling method, wherein the gout comprises primary gout and secondary gout. It is found that the herba lycopi flavone can significantly reduce the activity of xanthine oxidase in vitro and can effectively reduce the serum uric acid level of a hyperuricemia model mouse at the same time, and it is indicated that the herba lycopi flavone can be used for preparing the anti-hyperuricemia and/or anti-gout medicine. The invention provides a safe, effective and economical active substance for preparing medicines for preventing and/or treating hyperuricemia or/and gout diseases.

Description

technical field [0001] The invention relates to the application of Eupatorium flavonoid in the preparation of medicines, mainly relates to the application of Eupatorium flavone in the preparation of anti-hyperuricemia and anti-gout drugs, and belongs to the technical field of medicine. Background technique [0002] Gout is a joint disease caused by crystal formation of monosodium urate deposition, which is directly related to hyperuricemia caused by purine metabolism disorder and / or decreased uric acid excretion. [0003] Hyperuricemia refers to the concentration of uric acid in the blood of adults measured by the phosphotungstic acid reduction method is higher than 420 μmol / L in male blood, and the concentration of uric acid in female blood is higher than 357 μmol / L, or measured by uricase-peroxidase coupling The state of uric acid concentration in adult blood is higher than 420 μmol / L, which is caused by increased production and / or decreased excretion of uric acid in the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P19/06
CPCA61K31/352A61P19/06
Inventor 杜冠华周启蒙杨海光赵晓悦宋俊科张雯
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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