Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing artificial cell containing onion-shaped multilayer phospholipid membrane structure by one-step temperature control method

An artificial cell, onion-like technology, applied in the field of artificial cell model preparation with complex structures, to achieve the effect of large output, low cost, and easy operation

Active Publication Date: 2022-04-22
HARBIN INST OF TECH
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that it is difficult to directly construct an artificial cell model containing a multi-layered inner membrane structure under physiological conditions, and to provide a direct temperature control method in an aqueous solution of triacetin containing onion. A method for constructing artificial cells with multilayered phospholipid membranes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing artificial cell containing onion-shaped multilayer phospholipid membrane structure by one-step temperature control method
  • Method for preparing artificial cell containing onion-shaped multilayer phospholipid membrane structure by one-step temperature control method
  • Method for preparing artificial cell containing onion-shaped multilayer phospholipid membrane structure by one-step temperature control method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for preparing artificial cells containing an onion-like multilayer phospholipid membrane structure by a one-step temperature control method, the method is specifically as follows:

[0039] Step 1: use chloroform to prepare a DPPC solution with a concentration of 2 mg / mL, and add NBD-PE with a mass fraction of 2% as a fluorescent dye;

[0040] Step 2: Add 10 μL of the phospholipid chloroform solution prepared in Step 1 to the EP tube, volatilize the chloroform under a nitrogen atmosphere, add 1 mL of a mixed solution of triacetin and ultrapure water, the volume ratio of ultrapure water and triacetin is 100:6, using a digital vortex mixer for shear oscillation, the frequency is 1200rpm;

[0041] Step 3: Put the solution prepared in Step 2 at 20°C min -1 Raise the temperature to 60°C at the heating rate, keep the constant temperature for 20-30 minutes, and then set the temperature at 5-10°C·min -1 The cooling rate is lowered to room temperature to obtain monolay...

Embodiment 2

[0043] A method for preparing artificial cells containing an onion-like multilayer phospholipid membrane structure by a one-step temperature control method, the method is specifically as follows:

[0044] Step 1: Prepare a DPPC solution with a concentration of 5 mg / mL in chloroform, and add NBD-PE with a mass fraction of 2% as a fluorescent dye;

[0045] Step 2: Add 10 μL of the phospholipid chloroform solution prepared in Step 1 to the EP tube, volatilize the chloroform under a nitrogen atmosphere, add 1 mL of a mixed solution of triacetin and ultrapure water, the volume ratio of ultrapure water and triacetin is 100:6, using a digital vortex mixer for shear oscillation, the frequency is 1200rpm;

[0046] Step 3: Put the solution prepared in Step 2 at 20°C min -1 Raise the temperature to 60°C at the heating rate, keep the constant temperature for 20-30 minutes, and then set the temperature at 5-10°C·min -1 The cooling rate is lowered to room temperature, and artificial cells...

Embodiment 3

[0048] A method for preparing artificial cells containing an onion-like multilayer phospholipid membrane structure by a one-step temperature control method, the method is specifically as follows:

[0049] Step 1: use chloroform to prepare a DPPC solution with a concentration of 10 mg / mL, and add NBD-PE with a mass fraction of 2% as a fluorescent dye;

[0050] Step 2: Add 10 μL of the phospholipid chloroform solution prepared in Step 1 to the EP tube, volatilize the chloroform under a nitrogen atmosphere, add 1 mL of a mixed solution of triacetin and ultrapure water, the volume ratio of ultrapure water and triacetin is 100:6, using a digital vortex mixer for shear oscillation, the frequency is 1200rpm;

[0051] Step 3: Put the solution prepared in Step 2 at 20°C min -1 Raise the temperature to 60°C at the heating rate, keep the constant temperature for 20-30 minutes, and then set the temperature at 5-10°C·min -1 The cooling rate is lowered to room temperature, and artificial ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The average diameteraaaaaaaaaa
The average diameteraaaaaaaaaa
The average diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing an artificial cell containing an onion-shaped multilayer phospholipid membrane structure by a one-step temperature control method, and belongs to the technical field of preparation of an artificial cell model with a complex structure. The method comprises the following steps: preparing a solution with the concentration of 2-30mg / mL by using chloroform as a solvent and an element with amphipathy and temperature response self-assembly behavior as a solute, and adding NBD-PE as a fluorescent coloring agent; the method comprises the following steps: adding an element chloroform solution into a container, volatilizing chloroform in an inert gas environment, adding a mixed solution of glyceryl triacetate and ultrapure water, and carrying out shear oscillation on the solution; and heating the solution to 50-65 DEG C at a certain heating rate, keeping the constant temperature for 20-40 minutes, and cooling to room temperature at a certain cooling rate. The onion-shaped multilayer phospholipid artificial cell is prepared by a one-step method under physiological conditions by utilizing the self-assembly performance of phospholipid molecules; the onion-shaped multilayer phospholipid artificial cell has a stable multilayer inner membrane structure and good biocompatibility; the size and the number of layers of the onion-shaped multilayer phospholipid artificial cell can be regulated and controlled.

Description

technical field [0001] The invention belongs to the technical field of preparation of artificial cell models with complex structures, and in particular relates to a method for preparing artificial cells with an onion-like multilayer phospholipid membrane structure by a one-step temperature control method. Background technique [0002] Cells are the basic structural and functional units that constitute known organisms in nature, and are the most basic "life" entities in living organisms, representing the ultimate model for the development of modern life. However, due to the complexity of the cell structure, as well as the constant chemical reactions and material exchanges in the cell, it is difficult to study the cell structure and function. Therefore, researchers have adopted a bottom-up strategy to construct a variety of artificial cell models, mainly including semipermeable membrane spherical assemblies, polymer capsules, protein capsules, and phospholipid capsules. The c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01J13/06
CPCB01J13/06
Inventor 王磊李宏凯孙照玲黄鑫
Owner HARBIN INST OF TECH