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Molecular marker ricinoleic acid related to azoospermia in semen as well as detection method and application of molecular marker ricinoleic acid

A molecular marker, ricinoleic acid technology, applied in the fields of analytical chemistry and clinical medicine, can solve problems that have not received corresponding attention, achieve the effects of delaying and preventing disease progression, improving sensitivity and specificity, and being easy to detect

Active Publication Date: 2022-04-26
苏州南医大创新中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of metabolomics analysis of seminal plasma metabolic small molecules in the diagnosis and monitoring of azoospermia has not received corresponding attention

Method used

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  • Molecular marker ricinoleic acid related to azoospermia in semen as well as detection method and application of molecular marker ricinoleic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] 1. Research object selection and grouping basis

[0082] The subjects of this part of the study were first-diagnosed azoospermia cases and normal controls with normal semen parameters from the Affiliated Hospital of Nanjing Medical University and the Reproductive Medicine Center of the Sixth Affiliated Hospital of Sun Yat-sen University. The research content and the informed consent form were approved by the Ethics Committee of Nanjing Medical University and complied with the requirements of relevant laws and regulations. Cases and controls signed the informed consent after understanding the contents. All subjects underwent a complete physical examination and completed a questionnaire including basic personal information, living habits, occupational and environmental exposures, genetic risk factors, sexual and reproductive function, disease history, and physical activity. Specific sample classification criteria are as follows:

[0083] A total of 146 cases were includ...

Embodiment 2

[0116] Embodiment 2 ricinoleic acid is to the diagnosis of azoospermia

[0117] According to the above-mentioned UPLC-Q active MS metabolomics method, the inventors detected ricinoleic acid in the semen samples of 62 cases and 84 controls, so as to draw the ROC curve and evaluate the sensitivity and specificity of the prediction, and then evaluate the detection of ricinoleic acid in the semen. The ability of this metabolic small molecule level to assess azoospermia.

[0118] See figure 1 , the sensitivity of ricinoleic acid was 88.1%, the specificity was 96.8%, and the area under the ROC curve was 0.972, so ricinoleic acid has a good ability to diagnose azoospermia.

Embodiment 3

[0119] Example 3 is used for the production of azoospermia semen ricinoleic acid detection and diagnostic kit

[0120]Firstly, the metabolic small molecules with high abundance in normal control and azoospermia semen were determined by UPLC-Q exactive MS. Then, the metabolic small molecules related to azoospermia were screened by the metabolomics technology based on UPLC-Q active MS, as the diagnostic index of azoospermia. Finally, the number of corresponding metabolic small molecules screened out is controlled to 1, which is an optimized simplification based on the pre-experiment. This kit includes a batch of reagents and consumables for the detection of small metabolic molecules. Among them, the standard of ricinoleic acid is used for the qualitative and quantitative analysis of small metabolic molecules, and the internal standard A is used for auxiliary analysis: creatinine, valine, niacin, thymidine , glutaric acid, L-phenylalanine, N-acetyl p-aminophenol, hippuric acid e...

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Abstract

The invention relates to a molecular marker ricinoleic acid related to azoospermia in semen as well as a detection method and application of the molecular marker ricinoleic acid, and belongs to the technical field of analytical chemistry and clinical medicine. Metabolic small molecules in normal control and azoospermia semen are compared by adopting UPLC-Qexactive MS (Ultra Performance Liquid Chromatography-Quantitative Exactive MS), ricinoleic acid which can be used for evaluating whether the semen suffers from azoospermia and has diagnostic value exists in the semen and application of UPLC-Qexactive MS for detecting the ricinoleic acid is found, and an azoospermia diagnosis and monitoring kit convenient for clinical application is developed. Metabolic small molecules are novel biomarkers, are highly associated with disease outcomes, and are stable, non-invasive, easy to detect and accurate in quantification. The ricinoleic acid provided by the invention can be used as a diagnostic marker of azoospermia, provides a basis for further deep examination of clinicians, provides support for quickly and accurately mastering disease states and severity of illness states of patients and timely taking prevention and treatment schemes, and delays and prevents disease progression.

Description

technical field [0001] The invention relates to a molecular marker ricinoleic acid related to azoospermia in semen, a detection method and application thereof, and belongs to the technical fields of analytical chemistry and clinical medicine. Background technique [0002] In 1988, the World Health Organization (WHO) first proposed the concept of reproductive health, that is, the complete and harmonious physiological, psychological and social conditions of human beings and their individuals in the structure, function and behavior of reproductive-related organisms throughout life. health status. Infertility is a prevalent couple problem worldwide, with an estimated 4-17% of couples seeing a doctor for infertility treatment, but it is generally believed that many more infertile couples do not go to a clinic. Surveys show that the male factor is the main or only factor accounting for about 20-50% of the causes of infertility. The incidence of azoospermia accounts for 10%-20% o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72G01N2030/027
Inventor 沙家豪袁艳陈敏健杨晓玉王嘉琛姚莹
Owner 苏州南医大创新中心