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Lactic acid producing transgenic hydrogenophilic bacteria

A technology of hydrogenophilic bacteria and lactate dehydrogenase, applied in the direction of bacteria, genetic engineering, plant genetic improvement, etc., can solve the problems of putting into practice the industrialized method of producing lactic acid, and achieve the effect of reducing emissions and strengthening recycling

Pending Publication Date: 2022-04-29
UTILIZATION OF CARBON DIOXIDE INST CO LTD
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Problems solved by technology

However, the application of electric current is required to produce a sufficient amount of lactic acid, so the method using Hydrogenobacillus thermophiles as a host has not yet been put into practical use as an industrial method for producing lactic acid

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  • Lactic acid producing transgenic hydrogenophilic bacteria
  • Lactic acid producing transgenic hydrogenophilic bacteria
  • Lactic acid producing transgenic hydrogenophilic bacteria

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Embodiment

[0208] Next, the present invention will be described in further detail with reference to examples, but the present invention is not limited to these examples.

[0209] ( 1) Acquisition of streptomycin-resistant strains

[0210] Hydrogenophilus thermoluteolus TH-1 strain (NBRC14978) (hereinafter sometimes referred to as "TH-1 strain") was inoculated using a platinum ring into which was placed 5 mL of A liquid medium [3.0 g (NH 4 ) 2 SO 4 , 1.0g KH 2 PO 4 , 2.0g K 2 HPO 4 , 0.25g NaCl, 0.014g FeSO 4 ·7H 2 O, 0.5g MgSO 4 ·7H 2 O, 0.03g CaCl 2 , 4.0mg MoO 3 , 28mg ZnSO 4 ·7H 2 O, 2.0 mg CuSO 4 ·5H 2 O, 4.0 mg H 3 BO 3 , 4.0mgMnSO 4 ·5H 2 O and 4.0 mg CoCl 2 ·6H 2 O(pH 7.0)] in a test tube filled with H 2 :O 2 :CO 2 =7.5:1:1.5 mixed gas, and cultured with shaking at 50°C. After 24 hours, the culture solution was applied to A solid medium containing 500 μg / mL streptomycin [3.0 g (NH 4 ) 2 SO 4 , 1.0g KH 2 PO 4 , 2.0g K 2 HPO 4 , 0.25gNaCl, 0.014g Fe...

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Abstract

In a bacterium of the genus Hydrogenobacteria, when one or more of three lactic acid-utilizing enzyme genes on the genome of the bacterium of the genus Hydrogenobacteria are disrupted and a lactic dehydrogenase gene and / or a malic acid / lactic dehydrogenase gene are introduced thereto, lactic acid production capacity is significantly improved. The inventors of the present invention have identified three lactic acid-utilizing enzyme genes of said bacteria of the genus Hydrophile. In addition, by introducing a lactic acid permease gene into the transgenic bacterium, the lactic acid production capacity is further improved. The transgenic bacteria efficiently produce lactic acid by using carbon dioxide as a sole carbon source. That is to say, the transgenic bacterium can efficiently produce raw materials of biodegradable plastics, and solves the problem of global warming caused by increase of carbon dioxide at the same time.

Description

technical field [0001] The present invention relates to a recombinant bacterium of the genus Hydrogenophilus having the ability to produce lactic acid and a method for producing lactic acid using the bacterium. Background technique [0002] The Paris Agreement, adopted in 2015, stipulates that global greenhouse gas emissions should be rapidly reduced. Under the agreement, Japan set a target of reducing its emissions of greenhouse gases such as carbon dioxide and methane by 26% by 2030 compared to 2013 levels. [0003] Worldwide, the production of most chemicals is dependent on petroleum resources, exacerbating the problem of increasing greenhouse gas emissions. Therefore, weaning off dependence on petroleum is an ideal strategy for chemical production, and research and development of biorefineries to produce green chemicals from biomass are being seriously pursued in various countries. However, saccharification of biomass used as a raw material for microbial fermentation r...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12P7/56C12N15/53
CPCC12P7/56C12N9/0006C12N1/205C12R2001/01C12Y101/01027C12Y101/01037
Inventor 汤川英明大谷直人
Owner UTILIZATION OF CARBON DIOXIDE INST CO LTD
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