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Lactobacillus reuteri and application thereof in preparation of preparation for preventing cat diarrhea

A technology of Lactobacillus reuteri and diarrhea, applied in the field of Lactobacillus reuteri and its application in the preparation of preparations for preventing cat diarrhea, can solve the problem of mixed sources of probiotic products, uneven quality of micro-ecological products, and pet health care functions Unclear and other problems, to achieve a strong antibacterial effect

Active Publication Date: 2022-05-06
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the lack of systematic research on probiotics for cats in my country, the sources of probiotic products on the market are mixed, and the probiotic health care functions for pets are not clear. The supervision is relatively loose, resulting in the uneven quality of micro-ecological products on the market

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  • Lactobacillus reuteri and application thereof in preparation of preparation for preventing cat diarrhea
  • Lactobacillus reuteri and application thereof in preparation of preparation for preventing cat diarrhea
  • Lactobacillus reuteri and application thereof in preparation of preparation for preventing cat diarrhea

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Experimental program
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Embodiment

[0025] 1. Materials

[0026] 24 pieces of feces from healthy cats; put them into sterile sampling tubes and put them into a refrigerator immediately (temperature below 4°C), and transfer the samples to a -80°C refrigerator within 8 hours for storage.

[0027] Culture medium: MRS and BHI medium (purchased from Oboxin Biological Co., Ltd.).

[0028] Main reagents: Gram staining reagent, bacterial genomic DNA extraction kit (Tiangen), lysozyme (Tiangen), lysozyme buffer (Shenggong), universal primer 27F (BGI), universal primer 1492R (BGI ), agarose, drug-sensitive paper (gentamicin 10 μg / tablet, enrofloxacin 10 μg / tablet, ampicillin 10 μg / tablet, penicillin 10 U / tablet, cephalothin 30 μg / tablet, vancomycin 30 μg / tablet, Ciprofloxacin 5 μg / tablet was purchased from Hangzhou Microbiological Reagent Co., Ltd.).

[0029] Indicator strains: Shigella (ATCC25957), Salmonella (CGMCC1.1869) and Campylobacter jejuni (ATCC33291) (purchased from Qingdao Haibo Biotechnology Co., Ltd.).

[...

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Abstract

The invention discloses lactobacillus reuteri, and the preservation number of the lactobacillus reuteri is CGMCC (China General Microbiological Culture Collection Center) NO: 24339. The compound has good acid and bile salt resistance and intestinal epithelial cell adhesion, is sensitive to various antibiotics, and has a bacteriostatic effect on common pathogenic bacteria causing cat diarrhea, such as salmonella, campylobacter jejuni and shigella when being used for preparing a preparation for preventing cat diarrhea; cat animal experiments show that the strain has a good prevention effect on diarrhea caused by shigella, and has the potential of being developed into a microecological preparation for preventing cat diarrhea.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, to a Lactobacillus reuteri and its application in preparing preparations for preventing cat diarrhea. Background technique [0002] Probiotics have been proven to regulate the intestinal flora of cats, enhance the immunity of cats, improve the growth performance of young cats, and relieve the inflammatory response of cats. [0003] Due to the lack of systematic research on probiotics for cats in my country, the sources of probiotic products on the market are mixed, and the probiotic health care functions for pets are not clear. The relatively loose supervision has resulted in the uneven quality of micro-ecological products on the market. Therefore, it is of great application value and market prospect to isolate and identify pet-derived lactic acid bacteria from the intestinal tract of healthy cats, evaluate the probiotic function of the purified lactic acid bacteria strains, screen...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/40A61K35/747A61P31/04A61P1/12C12R1/225
CPCA23K10/18A23K50/40A61K35/747A61P31/04A61P1/12A23V2400/173Y02A50/30
Inventor 逄晓阳吕加平汪家琦潘丽娜康文丽吴忠坤颜卫彬
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI