Application of Tilorone in prevention/treatment of African swine fever virus infection

An African swine fever virus and application technology, applied in the field of medicine, can solve the problems of research and disclosure without inhibitory/therapeutic effects, and achieve the effects of excellent oral bioavailability, high selectivity coefficient, and multiple modes of administration

Pending Publication Date: 2022-05-13
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the prior art, there is no research and disclosure on the inhibitory / therapeutic effect of Tilorone / Tilorone hydrochloride on African swine fever virus

Method used

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  • Application of Tilorone in prevention/treatment of African swine fever virus infection
  • Application of Tilorone in prevention/treatment of African swine fever virus infection
  • Application of Tilorone in prevention/treatment of African swine fever virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Study on Inhibition of African Swine Fever Virus Infection Stage by Tilorone Hydrochloride

[0036] Take 2×10 5 Cells / well were inoculated on lysine-coated 96-well plates and incubated in a cell culture incubator for 24 hours. Infect with 1MOI, and add Tyrolone (such as figure 2 indicated), at a concentration of 10 μM. At the same time, in order to exclude the direct toxic effect of the drug on the virus, the virus and Tyrolone were mixed evenly, and the final concentration of the drug was 10 μM. After incubation at 37°C for 1 hour, the mixture was diluted 100 times, and the cells were infected. At this time, the infection dose was 1 MOI. . Collect samples within 24 hours to detect virus titer HAD 50 and genome copy number. see results image 3 . It shows that Tilorone hydrochloride has obvious inhibition to African swine fever virus.

Embodiment 2

[0038] In vitro activity and cytotoxicity experiments of Tilorone hydrochloride inhibiting the replication of African swine fever virus

[0039] By measuring CC 50 To evaluate the toxicity of Tilorone hydrochloride to cells: with 4×10 5 Porcine alveolar macrophages (porcine alveolar macrophages, PAM) were inoculated in a 96-well plate at a density of one per well and incubated for 24 hours.

[0040] Drug configuration and dilution method are as follows: Tirolone hydrochloride is dissolved in DMSO, is configured into a 1mM solution, and then diluted to 0.2mM with 1640 medium containing 10% FBS and 2% double-antibody SP (penicillin and streptomycin) as the initial concentration , and then sequentially carried out 2-fold dilution to form 8 gradients, and each gradient was repeated for 3 replicate wells.

[0041] After the cells were cultured for 24 hours, discard the cell culture supernatant, add 100 μL / well of the diluted drug, and place it in the cell culture incubator for 72...

Embodiment 3

[0047] Pharmacokinetics of Tilorone Hydrochloride in Miniature Bama Pigs

[0048] Three healthy male and three female Bama pigs were given Tilorone hydrochloride by intravenous injection and gavage respectively, and the blood drug concentration of the test compound Tilorone hydrochloride in plasma was quantitatively determined by liquid chromatography-tandem mass spectrometry, and the pharmacokinetic parameters were calculated to investigate the Pharmacokinetic characteristics of compounds in miniature Bama pigs.

[0049] 3.1 Experimental materials:

[0050] animal species Pig animal strain mini parmesan pig animal age 0.5-1years Animal sex and number 6, half male / female animal weight 11.0-14.0kg animal source Jiangsu Langyue Biotechnology Co., Ltd. Certificate No. 3260374713

[0051] 3.2 Animal feeding:

[0052] Animals were kept in single cages in the Experimental Animal Center of Suzhou Laio Biotechnology Co., Ltd. ...

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Abstract

The invention relates to the technical field of medicines, in particular to application of Tilorone to prevention/treatment of African swine fever virus infection. The invention relates to application of telolone and salt forms, hydrates, solvates and isotope substitutes thereof in preparation of drugs for preventing or treating African swine fever viruses. The invention has the following advantages: (1) the bioavailability is high, and the half-life period is long; and (2) the maximum tolerance dose is high, the selectivity coefficient is high, and the safety is high. And (3) the activity of preventing/resisting African swine fever virus infection is strong. And (4) the drug delivery modes are multiple, and the application range is wide. And (5) the composition can be combined for use and has a good effect of preventing/treating African swine fever virus infection.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of Tilorone for preventing / treating African swine fever virus infection. Background technique [0002] Tilorone hydrochloride is a drug with decades of research history. The drug is approved in Russia, Georgia and other countries for adult viral hepatitis A / B / C, herpes and cytomegalovirus infection; as infectious allergy and viral Encephalomyelitis (multiple sclerosis, leukoencephalitis, uveoencephalitis, etc.), part of genitourinary and respiratory chlamydia comprehensive therapy, and treatment and prevention of influenza and SARS; in children over 7 years old for influenza and Treatment and prevention of SARS. [0003] In addition, a new type of veterinary drug with Tilorone as an active ingredient was launched in Ukraine in 2008. In the prior art, it is disclosed that Tilorone has oral antiviral activity against infection with influenza virus A / B / A 2 Mice w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/138A61P31/20
CPCA61K31/138A61P31/20
Inventor 翁长江金锋郑君李婷婷魏文娟黄韬李江南黄丽张朝霞
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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