Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of composition of lactobacillus sake and stachyose in preparation of medicine for treating constipation

A composition, technology of Lactobacillus, applied in the field of medicine and health food science

Active Publication Date: 2022-05-24
ICDC CHINA CDC
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, drug treatment often has many side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of composition of lactobacillus sake and stachyose in preparation of medicine for treating constipation
  • Application of composition of lactobacillus sake and stachyose in preparation of medicine for treating constipation
  • Application of composition of lactobacillus sake and stachyose in preparation of medicine for treating constipation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Isolation, screening and identification of lactic acid bacteria

[0040] 1.1 Isolation of Lactobacillus sake furu2019

[0041] 1) Prepare MRS agar medium after autoclaving at 121°C for 15min, then cool it to 50°C, shake it gently, and pour it into a petri dish after mixing;

[0042] 2) Dissolve 1g of homemade fermented bean curd sample in 5ml of 1xPBS, and then take out 100ul of it and spread it on MRS medium;

[0043] 3) Incubate for 48h at 37℃, 0.5% CO2 environment;

[0044] 4) Pick wet, circular raised gray-white single colonies for transfer.

[0045] 1.2 Culture preservation

[0046] The laboratory uses the MRS medium containing 30% glycerol as the bacterial preservation solution for the cryopreservation of the strains. The methods are as follows:

[0047] 1) Sterilize the 2mL sterilization tube at 121℃ for 15min and prepare it for use;

[0048] 2) After the bacteria are continuously transferred on the MRS medium for 3 times, add 2ml of sterile bacteri...

Embodiment 2

[0062] Example 2. Evaluation of constipation improvement function of Lactobacillus sake furu2019

[0063] 1. Methods and evaluation indicators

[0064] 1. Animal experiment: 40 SPF grade 6-8 week old male ICR mice (24-26 g) were randomly divided into five groups (eight mice in each group) after adaptive feeding for seven days: blank control group, constipation model group, stachyose (2 kg per pack, provided by China Institute of Food and Fermentation Industry) intervention group (ST), Lactobacillus sake intervention group (LS), stachyose + Lactobacillus sake mixed intervention group (ST+LS). The blank control group and the model group were given 0.3 ml of PBS every day, the stachyose intervention group was given 0.3 ml of stachyose solution (the dose was 1.5 g / kg, about 35 mg per mouse), and the Lactobacillus sake intervention The group was given 0.3 ml of Lactobacillus sake (dose of 3.5 × 10 9 CFU), stachyose + Lactobacillus sake mixed intervention group was given 0.3 ml of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a lactobacillus probiotic strain, the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.22254, the preservation date is April 28, 2021, the preservation classification is named as Lactobacillus sakei (Lactobacillus sakei), and the preservation unit is China General Microbiological Culture Collection Center. The sequence of 16S rDNA of the strain is shown as SEQ ID NO: 1, the invention further discloses application of the strain and a composition of the strain and stachyose in preparation of food, health care products and medicines for improving digestive tract functions, and the strain is harmless to animals and has the efficacy of regulating and improving constipation.

Description

technical field [0001] The invention relates to a probiotic and its combined application with a prebiotic, belonging to the field of medicine and health food science. Background technique [0002] Slow transit constipation (STC) is a common type of constipation, and its clinical symptoms include hard and dry stools, difficulty defecation, and infrequent defecation. Usually, constipation is mainly concentrated in the elderly, women and people with high stress. In parts of Africa, the incidence of constipation is as high as 30%, and the main mechanism of STC is prolonged intestinal transit time due to slowing of intestinal peristalsis, prolonged bacterial fermentation time, and prolonged water absorption time. [0003] The pathogenesis of constipation is more complex. The mechanisms for relieving constipation mainly include stimulating intestinal peristalsis by regulating intestinal flora and producing short-chain fatty acids by competition; regulating intestinal peristalsis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A61K35/747A61K31/702A61P1/10A23L33/135C12R1/225
CPCA61K35/747A61K31/702A61P1/10A23L33/135A23V2002/00A23V2400/179A61K2300/00A23V2200/3204Y02A50/30
Inventor 任志鸿宋利琼肖玉春黄元铭
Owner ICDC CHINA CDC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com