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Novel healing-promoting collagen-based biomedical wound dressing and preparation method thereof

A biomedical and collagen technology, applied in the field of biomedical materials, can solve the problems of low molecular density of grafted heparin polysaccharides, and achieve the effects of promoting the growth of epithelial cells, improving the quality of wound healing, and promoting the production of granulation tissue

Pending Publication Date: 2022-05-27
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the combination of low-molecular-weight heparin and collagen-based materials is physical adsorption, and only a small amount of heparin molecules can undergo ionization and complexation with collagen molecules, so the density of grafted heparin polysaccharide molecules on the surface of the modified material obtained is low.

Method used

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  • Novel healing-promoting collagen-based biomedical wound dressing and preparation method thereof
  • Novel healing-promoting collagen-based biomedical wound dressing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Dissolving low molecular weight heparin: Weigh 3.5 g of nadroparin calcium and dissolve it in 50 mL of distilled water to obtain a nadroparin calcium solution;

[0055] (2) Add oxidant: 80 mL of 0.25 mol / L sodium periodate solution by weight was slowly added dropwise to the nadroparin calcium solution while stirring. Magnetic stirring for 48 h;

[0056] (3) Add ethylene glycol: slowly add 15 mL by weight of ethylene glycol dropwise, and after the dropwise addition, place it at 4°C and keep it in the dark under the condition of magnetic stirring for 30 min;

[0057] (4) Purification: put the product into a dialysis bag with a molecular weight cut-off of 500 Da, and immerse it in water for injection at 4 °C; first dialyze for 60 minutes, and discard the water for injection; then add water for injection at 4 °C for 4 hours, and discard the Remove water for injection; then dialyze in water for injection at 4°C for 72 hours, during which the water for injection is changed...

Embodiment 2

[0069] (1) Dissolving low molecular weight heparin: Weigh 0.5 kg of enoxaparin sodium and dissolve it in 50 mL of distilled water to obtain an enoxaparin sodium solution;

[0070] (2) Add oxidant: slowly add 2.5 kg by weight of 0.25 mol / L sodium periodate solution into the enoxaparin sodium solution dropwise while stirring. under stirring for 72 h;

[0071] (3) Add ethylene glycol: slowly add 0.25 kg of ethylene glycol dropwise, and after the dropwise addition, place it at 0°C and keep it away from light and stir for 1 h;

[0072] (4) Purification: put the product into a dialysis bag with a molecular weight cut-off of 2000 Da and immerse it in ultrapure water at 0 °C; first dialyze for 60 min, then discard the ultrapure water; then add ultrapure water at 0 °C for dialysis 4 h, discard the ultrapure water; then dialyze the ultrapure water at 0°C for 72 h, during which the ultrapure water is changed every 24 hours;

[0073] (5) Drying: take out the product after dialysis, and ...

Embodiment 3

[0084] (1) Dissolving low molecular weight heparin: Weigh 6 g of dalteparin sodium and dissolve it in 100 mL of distilled water to obtain a dalteparin sodium solution;

[0085] (2) Add oxidant: 200 mL of 0.25 mol / L sodium periodate solution by weight was slowly added dropwise to the dalteparin sodium solution while stirring. Stir for 48 h;

[0086] (3) Add ethylene glycol: slowly dropwise add 15 g of ethylene glycol by weight, after the dropwise addition, place it at 8°C and keep it away from light and stir for 1 h;

[0087] (4) Purification: put the product into a dialysis bag with a molecular weight cut-off of 1000 Da, and immerse it in water for injection at 8 °C; first dialyze for 60 min, and discard the water for injection; then add water for injection at 8 °C for 4 hours, and discard Remove the water for injection; dialyze the water for injection at 8°C for 72 hours, during which the water for injection is changed every 24 hours;

[0088] (5) Drying: take out the produ...

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Abstract

The invention discloses a preparation method of a novel healing-promoting collagen-based biomedical wound dressing, which comprises the following steps: selectively oxidizing o-dihydroxyl in low-molecular heparin by using sodium periodate to prepare oxidized low-molecular heparin, and crosslinking the oxidized low-molecular heparin with a collagen-based material to prepare the oxidized low-molecular heparin crosslinked modified collagen-based material. According to the modified collagen-based material prepared by the invention, the biocompatibility and healing promotion performance of the collagen and the growth factor expression promotion and cell adhesion proliferation promotion of the low-molecular heparin generate a synergistic effect, so that the extracellular matrix microenvironment of local tissues of a wound surface can be effectively improved, reepithelization and tissue repair of the wound surface are promoted, and the wound surface is not damaged. When being used as a wound dressing, the collagen-based biomedical wound dressing not only has excellent hygrothermal stability, mechanical properties, hydrophilicity and cytocompatibility, but also has remarkable healing promoting performance and scar hyperplasia inhibition, is a novel collagen-based biomedical wound dressing capable of promoting healing, and can be widely applied to the field of biomedical materials such as skin wound repair and the like.

Description

technical field [0001] The invention relates to a preparation method of a novel collagen-based biomedical wound dressing for promoting healing, and also relates to the application of the collagen-based biomedical wound dressing for promoting healing prepared according to the method, belonging to the field of biomedical materials. Background technique [0002] The skin is the largest organ of the human body, which can protect the human body from external aggression and maintain the metabolism in the body. Various physical, chemical and biological stimulations of the external environment will cause the human skin to be damaged to varying degrees. The healing of injured wounds is a dynamic and complex process. Factors such as wound type, pathological conditions and dressing types will affect the healing speed and quality of wounds (Meng Zhaogang, Zhang Zixuan. Research progress in medical functional dressings[J]. PLA Journal of Preventive Medicine, 2020, 38(03): 88-89+93). As ...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L15/44A61L26/00C08B37/10
CPCA61L26/0033A61L26/0066A61L15/325A61L15/44C08B37/0075A61L2300/232A61L2300/412A61L2300/41C08L89/00
Inventor 但年华冯荣欣陈一宁
Owner SICHUAN UNIV