Preparation method of silk fibroin-based strong hydrogel capable of rapidly gelling
A technology of silk fibroin and hydrogel, which is applied in the field of biomaterials, can solve the problems of long gelation time and complicated preparation process, shorten the gelation time, have good biocompatibility, and achieve reliable The effect of control equipment
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[0033] The preparation method of the fast-gel silk fibroin-based strong hydrogel provided by the present invention comprises the following steps:
[0034] S1. Preparation of silk fibroin solution: after the degummed silk is cleaned with deionized water, it is dried at 60 ° C to obtain dry silk; the above-mentioned dry silk is dissolved and subjected to dialysis treatment to obtain silk fibroin. solution I;
[0035] S2. Preparation of silk fibroin-based hydrogel: adding a cross-linking agent to the silk fibroin solution I prepared in step S1 for cross-linking treatment to obtain a chemically cross-linked silk fibroin solution II, and then adding a cross-linking agent to the silk fibroin solution I prepared in step S1. The protein solution II and the gelatin solution are mixed uniformly to obtain the mixed solution III; after the mixed solution III is allowed to stand at low temperature, the silk fibroin / gelatin hydrogel is prepared.
[0036] S3, soak the silk fibroin / gelatin h...
Embodiment 1
[0046] Immerse 100 g of silk in 5 L of sodium carbonate solution with a mass fraction of 0.1%, and boil it for 30 minutes at 98 to 100 ° C. After each boiling, take out the cocoons and wash them with deionized water; The silkworm cocoons were added to 5L of sodium carbonate solution with a mass fraction of 0.1%, and the above steps were repeated three times until the silkworm cocoons were degummed, washed fully with deionized water, and dried in an oven at 60°C for 72 hours to obtain pure silk fibroin fibers.
[0047] Next, 5 g of the pure silk fibroin fibers prepared above were dissolved in 25 mL of lithium bromide solution to obtain a silk fibroin solution with a mass fraction of 5%, and then the silk fibroin solution was dialyzed at 4 °C for 72 hours; In the fibroin solution, 20% of the silk fibroin solution mass of MES, 20% of the silk fibroin solution mass of NHS, and 10% of the silk fibroin solution mass of EDC were added as cross-linking agents for chemical cross-linking...
Embodiment 2
[0049] The only difference between Example 2 and Example 1 is that the prepared silk fibroin / gelatin hydrogel is not 4 ) 2 SO 4 For soaking in the solution, the remaining steps are basically the same as those in Example 1, and will not be repeated here. The SEM image of the prepared hydrogel is shown in figure 2 As shown in the figure, it can be seen from the figure that the hydrogel has a three-dimensional pore-like structure, and the pore size is uniform. Compared with the hydrogel prepared in Example 1, the pore size has increased.
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