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Beauveria bassiana with function of infecting young silkworms, fungicide, application of fungicide and preparation method of silkworm larva

A technology of Beauveria bassiana and Beauveria bassiana, applied in the field of microorganisms, can solve problems such as shortage of raw materials, difficulty in quality assurance, and uneven quality of Beauveria bassiana

Active Publication Date: 2022-05-27
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the silkworm larvae mainly comes from the collection of dead silkworms in the process of raising silkworms. However, with the development of silkworm disease control technology, the occurrence of silkworm mortis has been effectively controlled, resulting in limited sources of silkworms, shortage of raw materials, and market prices. Continuously rising, the economic benefits are very significant
At the same time, there are good and bad phenomena in the collected white silkworms from production, and the quality is difficult to guarantee
Although there is a small amount of technical means for artificially cultivating B. larvae in the prior art, because B. larvae is a complex of bacteria and insects, different strains have an impact on the yield and quality of B. larvae. Both the quality and output of silkworms are restricted to a certain extent.

Method used

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  • Beauveria bassiana with function of infecting young silkworms, fungicide, application of fungicide and preparation method of silkworm larva
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  • Beauveria bassiana with function of infecting young silkworms, fungicide, application of fungicide and preparation method of silkworm larva

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preparation example Construction

[0049] The present invention also provides a preparation method of the silkworm, comprising the following preparation steps: spraying the bacterial agent described in the above technical scheme on the back of the silkworm body to obtain the silkworm. In the present invention, the bacterial agent is sprayed on the back of the silkworm body to obtain the dead silkworm body. After the dead silkworm body is obtained, in the present invention, the dead silkworm body is preferably transferred to the silkworm base, and cultured until the silkworm body becomes rigid, and white mycelial conidia grow to obtain the white silkworm. The temperature of the culture in the present invention is preferably 24-28°C, more preferably 25°C; the humidity of the culture is 90-98%, more preferably 96%. The silkworm body of the present invention preferably includes young silkworms, and more preferably includes fifth-instar young silkworms. The variety of silkworm body described in the present inventio...

Embodiment 1

[0054] Isolation and Identification of Beauveria bassiana Bb1003

[0055] The source of the white silkworm for the test: On June 3, 2021, the experimental silkworm was found among the silkworm breeding farmers in Ciying Town, Yangcheng County, Jincheng City, Shanxi Province. The young silkworms are Qiufeng Baiyu, and they were fed to 5th instar silkworms and tested. The temperature for culturing the young silkworms was 26°C ± 1°C, the relative humidity was 60wt.%~70wt.%, and the photoperiod was 16L:8D (lighting time was 16h). , the dark time is 8h), 5-year-old silkworms were obtained.

[0056] The medium is a spore-forming medium. The formula is: sucrose 15g, yeast powder 5g, peptone 2g, silkworm chrysalis powder 3g, rice flour 2.5g, corn flour 2.5g, lactose 3g, cotton kernel powder 1g, boric acid 0.1g, magnesium sulfate 1g, sulfuric acid Copper 0.05g, potassium dihydrogen phosphate 2g, zinc sulfate 0.05g, ferrous sulfate 0.05g, manganese sulfate 0.02g, agar 12g, add water to...

Embodiment 2

[0071] Determination of optimal concentration of Beauveria bassiana Bb1003 for infection

[0072] Beauveria bassiana Bb1003 was prepared with 1% Tween-80 in volume percentage to make the concentration of 1×10 5 pc.mL -1 , 1×10 6 pc.mL -1 , 1×10 7 pc.mL -1 , 1×10 8 pc.mL -1 , 1×10 9 pc.mL -1 The spore suspension was sprayed with the above-mentioned different concentrations of the spore suspension on the back of the 5th instar silkworms with similar individual length and activity using an ultra-low volume spraying can, and placed in a culture laboratory at 26±1°C and a humidity of 90wt.%. , feeding three times a day, feeding enough mulberry leaves. Each concentration was treated with 500 larvae, and each concentration was repeated 3 times. The observation was continued for 7 days, and the number of larvae rigidified was recorded every day, and the optimal concentration of infection was determined according to the rigidity rate.

[0073] Table 1 shows the rigidity rates...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to beauveria bassiana with a function of infecting young silkworms, a fungicide, application of the fungicide and a preparation method of white muscardine silkworms. The Beauveria bassiana provided by the invention comprises Beauveria bassiana UMCM2, and is characterized in that the Beauveria bassiana UMCM2 can be used for preparing the Beauveria bassiana. The strain provided by the invention has high pathogenicity to young silkworms, has the advantages of high stiffness rate and high sporulation quantity, and has wide application prospects in production and research of white muscardine silkworms.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Beauveria bassiana with the function of infecting young silkworms, a bacterial agent and applications thereof, and a preparation method of Beauveria bassiana. Background technique [0002] Beauveria bassiana (Bals.) Vuill., the larvae (pupa) of the silkworm moth family, the silkworm Bombyx mori L., is infected and killed by Beauveria bassiana (Bals.) Vuill. or artificial inoculation. ) body, is a traditional Chinese medicine in my country, with a history of more than 2,000 years of drug use. The white silkworm is flat in nature, salty and acrid in taste. It has the functions of extinguishing wind and relieving spasm, promoting blood circulation and dredging collaterals, resolving phlegm and dispersing knots, etc. It can be used for the treatment of various diseases clinically. [0003] At present, Bombyx mori is mainly derived from dead and diseased silkworms ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01K67/04C12R1/645
CPCC12N1/14A01K67/04Y02A50/30
Inventor 杨宁王晓闻张向博李维宏
Owner SHANXI AGRI UNIV
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