Aeromonas salmonicida attenuated strain derived from turbot and application of aeromonas salmonicida attenuated strain

A technology for killing Aeromonas salmonids and turbot, applied to climate change adaptation, medical preparations containing active ingredients, bacteria, etc., can solve the problems of not being able to protect infection at the same time

Active Publication Date: 2022-05-27
烟台市海洋经济研究院(烟台市渔业技术推广站、烟台市海洋捕捞增殖管理站)
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that commercial vaccines against Aeromonas salmonicida obtained from fish infected with salmonids and trout cannot protect turbot well, and vaccines prepared from different subspecies cannot protect Aeromonas salmonicida at the same time. For infections caused by typical and atypical strains of Bacillus sp., it is necessary to prepare special vaccines for different isolation sources and different subspecies

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aeromonas salmonicida attenuated strain derived from turbot and application of aeromonas salmonicida attenuated strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The present invention will be specifically introduced below with reference to specific embodiments.

[0014] 1. Isolation and identification of strains

[0015] 1. Isolate strains

[0016] The diseased turbot samples were collected from the turbot breeding farm in Yantai, Shandong Province, and the individual weight was between 135 and 450 g. Affected turbot showed as follows: loss of appetite, black body color, hyperemia of fin rays, and abdominal enlargement. Necropsy findings: Pale red ascites in the abdominal cavity, intestinal swelling and congestion, splenomegaly.

[0017] The dying individuals of the diseased turbot with typical symptoms were taken, the internal organs were aseptically dissected, the liver, kidney and spleen were taken, washed with sterile water, ground, streaked on a nutrient agar plate supplemented with 2wt% NaCl, and cultured upside down at 28°C for 48h. The dominant colonies with the same morphology were picked for purification and culture...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a turbot-sourced aeromonas salmonicida attenuated strain and application thereof.The attenuated strain is a high-temperature derivative strain of aeromonas salmonicida for killing Japanese salmonella subspecies, namely aeromonas salmonicida AS-1, is classified and named as aeromonas salmonicida AS-1, is preserved in China Center for Type Culture Collection, the preservation address is Wuhan, China, and the preservation number is CGMCC NO.12487. The invention further discloses a method for preparing the turbot-sourced aeromonas salmonicida attenuated strain. The preservation date is February 18, 2022, and the preservation number is CCTCC (China Center For Type Culture Collection) NO: M 2022131. Through artificial infection, the aeromonas salmonicida AS-1 has low toxicity to scophthalmus maximus, a live vaccine prepared from the aeromonas salmonicida AS-1 is used for immune protection of the scophthalmus maximus, bacterial diseases caused by the fact that the aeromonas salmonicida kills subspecies Japanese salmonicida can be effectively prevented, and the aeromonas salmonicida AS-1 plays a positive role in reducing abuse of antibiotic drugs and guaranteeing quality safety of aquatic products.

Description

technical field [0001] The invention relates to an attenuated bacterial strain and application thereof, in particular to an attenuated Aeromonas salmonicida strain derived from turbot and application thereof, belonging to the technical field of pathogenic microorganism screening. Background technique [0002] Turbot belongs to the genus Florididae, and is an important species of northern factory farming. In recent years, with the continuous improvement of the intensification level of aquaculture, serious bacterial diseases have appeared in the breeding process, which restricts the green and high-quality development of the industry. [0003] Aeromonas salmonicida mainly infects cold-water salmon and trout, and in recent years, there have also been reports of infecting fish of the family Sushiidae, Cyprinidae, Soleidae, and Flounder. The Manual of Bacterial Taxonomy and Identification will include Aeromonas salmonicida ( Aeromonas salmonicida ) is divided into 5 subspecies, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
CPCA61K39/0208A61P31/04A61K2039/522A61K2039/552Y02A50/30
Inventor 王鹤贺加贝刘蓬王文豪陈坤黄华陈伟徐晓莹
Owner 烟台市海洋经济研究院(烟台市渔业技术推广站、烟台市海洋捕捞增殖管理站)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap