Aeromonas salmonicida attenuated strain derived from turbot and application of aeromonas salmonicida attenuated strain

Abstract

The invention discloses a turbot-sourced aeromonas salmonicida attenuated strain and application thereof.The attenuated strain is a high-temperature derivative strain of aeromonas salmonicida for killing Japanese salmonella subspecies, namely aeromonas salmonicida AS-1, is classified and named as aeromonas salmonicida AS-1, is preserved in China Center for Type Culture Collection, the preservation address is Wuhan, China, and the preservation number is CGMCC NO.12487. The invention further discloses a method for preparing the turbot-sourced aeromonas salmonicida attenuated strain. The preservation date is February 18, 2022, and the preservation number is CCTCC (China Center For Type Culture Collection) NO: M 2022131. Through artificial infection, the aeromonas salmonicida AS-1 has low toxicity to scophthalmus maximus, a live vaccine prepared from the aeromonas salmonicida AS-1 is used for immune protection of the scophthalmus maximus, bacterial diseases caused by the fact that the aeromonas salmonicida kills subspecies Japanese salmonicida can be effectively prevented, and the aeromonas salmonicida AS-1 plays a positive role in reducing abuse of antibiotic drugs and guaranteeing quality safety of aquatic products.

Description

technical field [0001] The invention relates to an attenuated bacterial strain and application thereof, in particular to an attenuated Aeromonas salmonicida strain derived from turbot and application thereof, belonging to the technical field of pathogenic microorganism screening. Background technique [0002] Turbot belongs to the genus Florididae, and is an important species of northern factory farming. In recent years, with the continuous improvement of the intensification level of aquaculture, serious bacterial diseases have appeared in the breeding process, which restricts the green and high-quality development of the industry. [0003] Aeromonas salmonicida mainly infects cold-water salmon and trout, and in recent years, there have also been reports of infecting fish of the family Sushiidae, Cyprinidae, Soleidae, and Flounder. The Manual of Bacterial Taxonomy and Identification will include Aeromonas salmonicida ( Aeromonas salmonicida ) is divided into 5 subspecies, ...

AI Technical Summary

Problems solved by technology

Studies have shown that commercial vaccines against Aeromonas salmonicida obtained from fish infected with salmonids and trout cannot protect turbot well, and vaccines prepared from different subspecies cannot protect Aeromonas salmonicida at the same time. For infections caused by typical and atypical strains of Bacillus sp., it is necessary to prepare special vaccines for different isolation sources and different subspecies

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