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Phaseolus calcaratus extract and application thereof in resisting obesity

A technology of extract and adzuki bean, which is applied in the application field of adzuki bean extract in anti-obesity, can solve problems such as different side effects, and achieve the effects of controlling body weight and reducing body fat rate.

Pending Publication Date: 2022-06-03
XINXIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the existing treatment methods are mainly drugs, which will cause different side effects with long-term intake

Method used

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  • Phaseolus calcaratus extract and application thereof in resisting obesity
  • Phaseolus calcaratus extract and application thereof in resisting obesity
  • Phaseolus calcaratus extract and application thereof in resisting obesity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Extraction of adzuki bean extract (water extraction method): The purchased adzuki bean is washed with clean water, soaked in the adzuki bean and distilled water in a ratio of 300g: 3L for 12 hours, and boiled with low heat until it is like a bean paste to obtain the adzuki bean extract; The extract was added to the full-price feed in proportion, dried in a blast dryer at 75°C for 12 hours, and then stored at -20°C for later use.

[0042] Twenty-eight, 6-week-old ICR male mice were purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd. and were raised in a constant temperature and humidity (temperature 22±1°C, humidity 50-60%), 12h / 12h day-night alternation (7 a.m. every day) : 00 lights on, 19:00 pm lights off) environment. The mice were randomly divided into 4 groups with 7 mice in each group. After one week of adaptation, the formal experiment was started, which were the normal diet group (Normal Diet, ND), the high-fat diet group (20.5% HFD, HD),...

Embodiment 2

[0047] Example 2 Sample Collection

[0048] (1) The experiment was carried out until the end of the 10th week. Three mice in each group were fixed, and fresh fecal samples were collected in sterile mouse cages and placed in sterile 1.5mL PE tubes; each sample to be tested was about 200 mg, immediately after collection. After being quick-frozen in liquid nitrogen, it was stored at -80°C until testing.

[0049] (2) After the mice were fasted for 12 hours, the mice were weighed and their body lengths were measured, and the mice's Lee's index was calculated.

[0050]

[0051] (3) Blood was collected from the mouse eyeball, and serum was separated; after cervical dislocation, the skull of the mouse was opened on an ice bath, and the hypothalamus tissue was quickly separated; Weigh and calculate the organ index; remove the fat in the abdominal cavity and testis and other tissues of each group of mice, weigh the wet weight of the adipose tissue, and calculate the body fat percent...

Embodiment 316

[0054] Example 3 16S rDNA gene sequencing

[0055] Total DNA was extracted from fresh fecal samples, and 16S rDNA gene high-throughput sequencing was used to analyze the types and contents of intestinal flora in mice. Using the microbial 16S rDNA gene as the target, using the Power Soil DNA isolation kit (Power DNAIsolation Kit) was used to extract DNA from mouse fecal samples, and beaded with the provided Power Bead tube for 20 minutes; after the sample was amplified by two-step PCR, quality control was carried out.

[0056]The specific experimental steps are as follows:

[0057] First, the first-stage PCR used bacterial 16S rRNA primers 515F and 806R (16S region V 4 primers), and the cycle conditions were 95 °C for 3 min, 98 °C for 20 s, 60 °C for 15 s, and 72 °C for 20 s for 25 cycles; extension at 72 °C for 5 min ; The second-stage PCR cycle conditions are 95°C for 3 min, then cycle 15 times at 95°C for 30s, 55°C for 30s, and 72°C for 30s, and extend at 72°C for 5min; t...

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Abstract

The invention discloses a phaseolus calcaratus extract and application of the phaseolus calcaratus extract in resisting obesity, and belongs to the technical field of weight-losing food. The phaseolus calcaratus extract prepared by a water extraction method has important influence on intestinal flora of high-fat diet mice, and the intestinal flora in the high-fat diet mice can be regulated and controlled by the phaseolus calcaratus extract, so that the body fat rate is reduced, and the body weight is controlled; meanwhile, a theoretical reference is provided for prevention and control and mechanism research of HFD-induced related diseases.

Description

technical field [0001] The invention belongs to the technical field of slimming foods, in particular to the application of an adzuki bean extract in anti-obesity. Background technique [0002] With the development of the economy, the improvement of people's living standards, the changes in lifestyle and dietary structure, the intake of HFD (high-fat diet) is increasing year by year. However, excessive HFD can increase the incidence of obesity and related diseases, such as brain damage, cardiovascular disease, non-alcoholic fatty liver disease, and colon cancer. Therefore, the establishment of effective preventive and therapeutic measures is a problem to be solved at present. [0003] However, the existing treatment methods are mainly drugs, which can cause different side effects with long-term intake. [0004] Therefore, how to provide an adzuki bean extract and its application in fighting obesity is an urgent problem to be solved in the art. SUMMARY OF THE INVENTION [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105
CPCA23L33/105A23V2002/00A23V2200/332A23V2250/21
Inventor 李雪华刘兴友崔金忠王小洁李鹏吴悦李雨轩高守鑫陈传双
Owner XINXIANG UNIV
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