Bifidobacterium longum for relieving insulin resistance and application thereof

A technology of Bifidobacterium longum and Bifidobacterium, applied in the field of microbiology, can solve problems such as heavy economic burden for patients, difficulty in maintaining blood sugar homeostasis, and adverse reactions in the digestive tract, so as to reduce liver triglyceride and inflammation levels, and relieve liver inflammation. Tissue damage, reducing the effect of insulin resistance index

Pending Publication Date: 2021-12-07
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the intervention of the drug metformin is mainly used to alleviate insulin resistance IR. Drug treatment has disadvantages such as difficulty in maintaining blood sugar stability, short drug effect time, and easy to cause adverse reactions in the digestive tract. Long-term treatment will bring heavy economic burden to patients.

Method used

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  • Bifidobacterium longum for relieving insulin resistance and application thereof
  • Bifidobacterium longum for relieving insulin resistance and application thereof
  • Bifidobacterium longum for relieving insulin resistance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0062] Preparation of activated medium (g / L): Components include carbon source: pectin 0.047, xylan 0.047, arabinogalactan, pullulan 0.04, soluble starch 0.392; nitrogen source: bactopeptone 24, tryptone 24. Inorganic salts: magnesium sulfate heptahydrate 0.5, potassium dihydrogen phosphate 2.5, sodium chloride 4.5, calcium chloride dihydrate 0.45, iron sulfate heptahydrate 0.005; bile salt 0.4, anaerobic cysteine ​​hydrochloride 0.2 and acid-base buffer (MES) 19.52. Firstly, the above ingredients are prepared, and the pH is adjusted to 6, followed by deoxygenation and sterilization (121° C., 15 min). After the sterilization, the medium was transferred to an anaerobic glove box, and 1 μg of high-temperature-labile heme, 1 μg of vitamin K3 (VK3) and 0.1 mL of vitamin mixture solution (Wolfe’s Vitamin Solution) were added to 1 L of culture medium through a 0.22 μm filter membrane. The base was deaerated overnight in an anaerobic glove box to obtain the activated liquid medium. ...

Embodiment 1

[0086] Example 1: Isolation and screening of Bifidobacterium longum NSP008

[0087] 1. Sample collection

[0088] Collect human feces samples of type Ⅱ diabetes in Shangqiu, Henan Province, place the samples in preservation tubes, add 5 times the weight of protective solution (preparation of protective agent: weigh cysteine ​​hydrochloride 1g / L, glycerol 200-300g / L, uniformly dissolved in PBS (1×), sterilized at 115-121°C for 15-20min), stored in an insulated box with dry ice, brought back to the laboratory, and immediately placed in a -80°C refrigerator to be separated and screened.

[0089] 2. Enrichment of fecal bacteria

[0090] Take the above-mentioned fecal bacteria liquid out of the -80°C refrigerator, and after thawing, centrifuge at low speed and low temperature (500g, 5min, 4°C) to obtain the supernatant, then pass through a 100μm filter membrane to remove impurities in the supernatant, and inoculate the supernatant fecal bacteria liquid into the activated culture ...

Embodiment 2

[0099] Example 2: Effects of Bifidobacterium longum NSP008 on body weight, body fat and diet of insulin-resistant mice on a high-fat diet

[0100] Specific steps are as follows:

[0101] 1. Preparation of Bifidobacterium longum NSP008 cryopreservation agent:

[0102] (1) Culture method: In a sterile anaerobic environment, the Bifidobacterium longum NSP008 bacterial classification was streaked on the MRS solid medium, cultivated under anaerobic conditions for 48 hours, after forming a single colony, pick a single colony, and It was inoculated into MRS liquid medium, and cultured anaerobically at 37° C. for 16-24 hours to reach a stationary phase, at which time the OD value was 1.0-1.4, and a seed solution was prepared.

[0103] (2) Preparation of protective agent: Weigh 1 g / L of cysteine ​​hydrochloride and 200-300 g / L of glycerol, dissolve them evenly in distilled water, and sterilize at 115-121° C. for 15-20 min.

[0104] (3) Preparation of refrigerant: Centrifuge (8000rpm,...

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Abstract

The invention discloses bifidobacterium longum for relieving insulin resistance and application of the bifidobacterium longum, and belongs to the technical field of microorganisms. The bifidobacterium longum NSP008 disclosed by the invention can be used for inhibiting the increase of the body weight and the body fat of an insulin-resistant mouse on the premise of not influencing the food intake; the glucose tolerance of an insulin-resistant mouse is improved, and meanwhile, the blood sugar, serum insulin and an insulin resistance index of the mouse are reduced; the insulin-resistant mouse liver triglyceride and inflammation level can be adjusted, and liver tissue damage can be relieved; the intestinal flora structure is improved, and the growth of intestinal probiotics is promoted. The bifidobacterium longum NSP008 disclosed by the invention is used for preparing a pharmaceutical composition and fermented food for relieving insulin resistance, and has a very wide application prospect.

Description

technical field [0001] The invention relates to a bifidobacterium longum for alleviating insulin resistance and an application thereof, belonging to the technical field of microbes. Background technique [0002] Insulin resistance (Insulin resistance, IR) refers to the condition that the biological effect of the target tissue is lower than that of healthy people under the action of normal doses of insulin, also known as "decreased insulin sensitivity". Insulin resistance IR is one of the main pathophysiological features of type Ⅱ diabetes mellitus, insulin resistance IR widely exists in obesity, non-alcoholic fatty liver, hyperlipidemia and other metabolic diseases. Diseases caused by insulin resistance IR, such as type Ⅱ diabetes and obesity, are widespread worldwide and have become a major public health issue of global concern. At present, the intervention of drug metformin is mainly used to alleviate insulin resistance IR. Drug treatment has disadvantages such as difficu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P5/50A61P3/10A23L33/135C12R1/01
CPCC12N1/20A61K35/745A61P5/50A61P3/10A23L33/135A23V2002/00A23V2400/533
Inventor 聂少平何卉珺孙永敢胡婕伦
Owner NANCHANG UNIV
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