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Marker composition for colorectal cancer detection and application thereof

A technology of colorectal cancer and composition, applied in the field of marker composition for colorectal cancer detection, can solve problems such as side effects of treatment, excessive anxiety of the crowd, economic loss, etc., to improve sensitivity and specificity, and realize early screening and early diagnosis The effect of avoiding overdiagnosis and treatment of problems

Active Publication Date: 2022-06-03
北京莱盟君泰国际医疗技术开发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that overdiagnosis and treatment of benign polyps will lead to excessive anxiety, economic loss and potential side effects of treatment for a large proportion of the population, especially for the frail elderly

Method used

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  • Marker composition for colorectal cancer detection and application thereof
  • Marker composition for colorectal cancer detection and application thereof
  • Marker composition for colorectal cancer detection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The embodiment of the present invention provides a marker composition for colorectal cancer detection, the marker composition is used for distinguishing benign and malignant intestinal polyps and detecting 57 plasma-free DNA methylation markers of colorectal cancer. at least one and the application. Specifically, it includes the following gene fragments with significant differential methylation in the plasma of colorectal cancer patients and intestinal polyps patients: chr13:46756082-46756083, chr16:29757360-29757361, chr16:29757375-29757376, chr1:211780424-211780425, chr16:29757350-29757351、 chr12:123707827-123707828、chr12:123707825-123707826、 chr1:211780438-211780439、chr20:55968287-55968288、 chr13:46756209-46756210、chr7:11208563-11208564、chr10:11207969-11207970、 chr13: 46756193-46756194、chr10:11207977-11207978、 chr5:115152492-115152493、chr5:115152479-115152480、 chr13:78493280-78493281、chr10:11207913-11207914、chr19:58095644-58095645、 chr6:393188-393189、chr16:86544859- ...

Embodiment 2

[0046] Example 2: Detection method of blood protein marker concentration and cfDNA methylation marker for colorectal cancer detection:

[0047] 1. The specific experimental steps of protein labeling detection are as follows:

[0048] 1. Sample processing: The serum should be separated within 24 hours after the venous blood collection. The hemolyzed and lipid blood samples should not be used for testing and must be resampled.

[0049] 2. Preparation before the test: first turn on the power of the instrument (MQ60 PLUS automatic chemiluminescence immunoassay analyzer), start initialization, and start the test 30 minutes after the instrument is turned on. Take out the separated serum samples. If the samples are frozen, they need to be thawed and equilibrated at room temperature for 30 minutes. At the same time, the kits and standards are taken out and equilibrated at room temperature for 30 minutes.

[0050] 3. Calibration: Add 500 μL of calibrator dilution solution to each of c...

Embodiment 3

[0154] In this example, based on 45 colorectal cancer adjacent tissue samples in the TCGA public database, 408 colorectal cancer tissue samples were mined to find 200 DNA methylation markers that were significantly differentially methylated in colorectal cancer samples. Design and synthesis of capture probes. Further, this example uses plasma samples from 203 patients with benign intestinal polyps and plasma samples from 230 patients with colorectal cancer to extract cfDNA, build a library, and sequence. Finally, 57 cfDNA methylation markers whose predictive performance and protein markers (CRP, CEA, CA19-9) were well complemented were screened out by random forest model for distinguishing benign intestinal polyps and colorectal cancer patients. The whole analysis process is as figure 1 shown.

[0155] like figure 2 As shown, this example discloses a combination of protein markers for detecting colorectal cancer, the AUC value of CRP alone for predicting colorectal cancer ...

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Abstract

The invention discloses a marker composition for colorectal cancer detection and application thereof, and belongs to the technical field of biological detection. According to the marker composition, three protein markers related to colorectal cancer diagnosis are combined with a plasma free DNA methylation biomarker, and an in-vitro blood sample can be used for distinguishing benign intestinal polyps from malignant colorectal cancer tumors. In view of noninvasiveness and convenience of an in-vitro blood sample, the method is suitable for large-scale popularization and application. Specific methylation sites related to the colorectal cancer in blood are detected, and in combination with a protein tumor marker detection result, the sensitivity and specificity of colorectal cancer detection are greatly improved, so that early screening and early diagnosis of the colorectal cancer are realized. The cfDNA methylation marker found by directly comparing in-vitro blood samples of a benign polyp and a colorectal cancer patient can distinguish benign and malignant polyp while detecting the colorectal cancer, and the problem of excessive diagnosis and treatment in the colorectal cancer screening process can be avoided to a great extent.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a marker composition for colorectal cancer detection and its application. Background technique [0002] According to the latest global cancer burden data released by the World Health Organization's International Agency for Research on Cancer (IARC) in 2020. How to effectively reduce the disease burden data of colorectal cancer is a major public health problem that needs to be solved urgently. [0003] Most colorectal cancers develop from adenomas, and the evolution from adenoma-dysplasia-carcinoma takes about 10-15 years. This time window provides a favorable opportunity for the prevention and early diagnosis of colorectal cancer. , which also makes colorectal cancer one of the few malignant tumors whose morbidity and mortality can be reduced by screening. A large number of studies and practices have shown that colorectal cancer screening and early diagnosis and ea...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574C12N15/11
CPCC12Q1/6886G01N33/57419C12Q2600/154
Inventor 徐建锋张慧珍李典格张泽跃张超李清华
Owner 北京莱盟君泰国际医疗技术开发有限公司
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