Pinellia tomentosa extract and method for separating clove resinol from Pinellia tomentosa extract

A yellow flower oil and extract technology is applied in the directions of active ingredients of heterocyclic compounds, medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, etc., to achieve the effect of enriching material base and efficient utilization

Pending Publication Date: 2022-06-24
SHAANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no relevant research and report on whether syringaresinol exists in C.

Method used

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  • Pinellia tomentosa extract and method for separating clove resinol from Pinellia tomentosa extract
  • Pinellia tomentosa extract and method for separating clove resinol from Pinellia tomentosa extract
  • Pinellia tomentosa extract and method for separating clove resinol from Pinellia tomentosa extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Candida oleifera extract

[0040] This embodiment provides an extract of S. japonica.

[0041] Its preparation method is as follows:

[0042] The dried and pulverized yellow flower oil point grass (picked in Zhashui County, Shaanxi Province in 2018, 10.0kg) coarse powder was extracted 4 times with 4.5 times the amount of 80% methanol dipping method at room temperature, and the successive intervals were 3, 5, 7, After 7 days, the filtrates were combined, methanol was recovered under reduced pressure, and concentrated to obtain 495.0 g of methanol-extracted extract. Disperse the extract in water, extract 5 times with ethyl acetate to obtain the ethyl acetate fraction, and concentrate to prepare the extract.

Embodiment 2

[0043] The separation of embodiment 2 syringaresinol

[0044] The present embodiment is further separated from the S. chinensis extract of Example 1 to obtain syringaresinol, such as figure 1 shown, the specific steps are as follows:

[0045] (1) Dissolve the ethyl acetate extract of the extract with methanol, mix the samples in a dry method, pass through a macroporous resin (Diaion HP-20 filler, 50%, 75%, 85% and 100% methanol by volume) Beijing Huideyi Technology Co., Ltd., 10×60cm, 5×6L) eluted, respectively obtained four fractions Fr.1-4, and collected the fraction Fr.2 (24.0g) eluted with methanol with a volume ratio of 75%;

[0046] (2) The fraction Fr.2 obtained in step (1) was separated by a medium pressure separation system, and 50%, 75% and 85% methanol were used in MCI column (MCI GEL CHP20P, Mitsubishi Chemical Corporation, Japan, 6×20cm, 6× 180ml) was separated to obtain three fractions Fr.2A-2C, and the fraction Fr.2C (1.8g) eluted with methanol with a volume r...

experiment example 1

[0052] Experimental Example 1 Antioxidant Experiment

[0053] 1. Experimental method:

[0054] Experimental group settings: The methanol solution of syringaresinol was used in the sample group, the methanol solution of the positive drug Trolox was used in the positive control group, and the methanol solvent was used in the blank control group. Weigh an appropriate amount of compound to prepare 1.00 mg·mL -1 Mother liquor, serially diluted to 0.50, 0.25, 0.13, 0.06, 0.03, 0.01 mg·mL -1 spare.

[0055] DPPH free radical scavenging experiment: Dissolve DPPH in methanol to prepare a 100 μM solution, dilute it to an absorbance of 0.7 ± 0.1 under a microplate reader, wrap it with tin foil and place it in the dark for later use. 50 μL of compounds (syringaresinol or positive drug Trolox) at different concentrations were added to 96-well plates in turn, the blank control group was added with the same dose of methanol solvent, and then 100 μL of prepared DPPH solution was added to e...

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a toadflower toadflower herb extract and a method for separating clove resinol from the toadflower toadflower herb extract. The invention provides an extract with effects of resisting oxidation and relaxing blood vessels. Through a column chromatography separation method, the active pharmaceutical ingredient clove resinol can be further separated from the extract. Due to the antioxidant effect and the vasodilatation effect, the extract and the syringaresinol have the potential of serving as drugs for treating or preventing hypertension, hyperlipidemia, lung cancer and related cancers, vascular dementia, diabetic erectile dysfunction, acute hyperuricemia or thrombotic diseases. The invention provides a new thought for research and development of the new drug, enriches the material basis of the tripterygium wilfordii, contributes to scientific and efficient utilization of the traditional Chinese medicinal material tripterygium wilfordii, and has higher application potential.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a S. japonica extract and a method for separating syringaresinol from the S. japonica extract. Background technique [0002] Tricyrtismaculata (D. Don) Machride, also known as S. horn seven, is a dry whole grass of the genus Tricyrtis maculata in the Liliaceae family. The whole plant and root of Cantaloupe is widely used in the treatment of migraine, fever, cough, stomach pain, tuberculosis cough and cerebral ischemic diseases, with remarkable curative effect. For example, Huanghuayou Diancao is one of the important components of Panlong Qi Tablets, a Chinese patent medicine for promoting blood circulation produced and sold by Shaanxi Panlong Pharmaceutical. [0003] The research on S. japonica has shown (Food Industry Science and Technology. v.39; No.402(10): 196-202. Chinese Medicinal Materials. 2016.39(2): 419-421. Chinese Invention Patent CN1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61K31/34C07D493/04A61P39/06A61P9/00
CPCA61K36/896A61K31/34C07D493/04A61P39/06A61P9/00
Inventor 孙静张小飞刘妍如王赟泽
Owner SHAANXI UNIV OF CHINESE MEDICINE
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