Preparation method of corn stigma polysaccharide and application of corn stigma polysaccharide in hypoglycemic products

A technology of corn silk polysaccharide and corn silk, which is applied in the extraction field, can solve the problems of large solvent consumption, complex solvent consumption, large solvent consumption, etc., and achieve the effects of improving extraction efficiency, good hypoglycemic effect and high yield.

Active Publication Date: 2022-06-28
JILIN BUSINESS & TECH COLLEGE
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method of the invention includes degreasing by soaking in ethanol and deproteinizing by trichloroacetic acid method, which needs to consume a large amount of solvent and will cause waste water pollution in certain cases.
[0005] Based on the above problems, the patent CN200910067588.1 discloses the application of corn silk polysaccharide in the preparation of drugs for the treatment of hyperlipidemia. The preparation of corn polysaccharides has been realized, and the prepared corn polysaccharides have better hypoglycemic and blood lipid-low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of corn stigma polysaccharide and application of corn stigma polysaccharide in hypoglycemic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method of corn silk polysaccharide, comprising the following steps:

[0038] (1) pre-treatment: sieving after corn silk cleaning, drying, pulverization, obtaining corn silk powder, the corn silk powder is 95% ethanol with mass fraction (the weight volume ratio of corn silk powder and ethanol is 1: 3g / mL) dipping, impurity removal, after repeating three times, obtain the corn silk powder after degreasing;

[0039] (2) extraction: adding water and composite protease (the selected weight ratio of the composite protease is 3:1 papain and ficin by weight ratio of the corn silk powder after the defatting, the weight ratio of the corn silk powder and water, the composite protease is 1: 15:0.001), soaked for 8 hours, heated to 55 °C for the first time for extraction, and after cooling, added bromelain and 10% ethanol solution by weight again (the weight-volume ratio of defatted corn silk powder to solution A and bromelain was 1:5:0.001), soaked for 12h, heated to...

Embodiment 2

[0043] A preparation method of corn silk polysaccharide, comprising the following steps:

[0044](1) pre-treatment: sieving after corn silk cleaning, drying, pulverization, obtaining corn silk powder, the corn silk powder is 95% ethanol with mass fraction (the weight volume ratio of corn silk powder and ethanol is 1: 4g / mL) dipping, impurity removal, after repeating three times, obtain the corn silk powder after degreasing;

[0045] (2) extraction: adding water and composite protease (the weight ratio of composite protease is 6:1 papain and ficin, the weight ratio of corn silk powder and water, composite protease is 1: 25:0.003), soaked for 12h, first heated to 50°C for extraction, and after cooling, added bromelain and 15% ethanol solution by weight again (the weight-volume ratio of defatted corn silk powder to solution A and bromelain was 1:10:0.002), soaked for 8h, heated to 50°C for the second time for extraction, the extraction step was carried out under ultrasonic condi...

Embodiment 3

[0049] A preparation method of corn silk polysaccharide, comprising the following steps:

[0050] (1) pre-treatment: sieving after corn silk cleaning, drying, pulverization, obtaining corn silk powder, the corn silk powder is 95% ethanol with mass fraction (the weight volume ratio of corn silk powder and ethanol is 1: 3.5g / mL) dipping, impurity removal, after repeating three times, obtain the corn silk powder after degreasing;

[0051] (2) extraction: adding water and composite protease to the degreasing corn silk powder material (complex protease selection weight ratio is papain and ficin protease of 5:2, the weight ratio of corn silk powder material and water, composite protease is 1: 20:0.002), soaked for 10h, heated to 50°C for the first time for extraction, and after cooling, added bromelain and 15% ethanol solution by weight again (the weight-volume ratio of defatted corn silk powder to solution A and bromelain was 1:8:0.001), soaked for 10h, heated to 45°C for the seco...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a preparation method of corn stigma polysaccharide and application of the corn stigma polysaccharide in a hypoglycemic product, and relates to the technical field of extraction.The preparation method specifically comprises the steps that 1, pretreatment is conducted, specifically, corn stigma is cleaned, dried, smashed and then sieved, corn stigma powder is obtained, the corn stigma powder is soaked with ethyl alcohol, impurities are removed, and after the operation is repeated for three times, degreased corn stigma powder is obtained; (2) extraction: adding water and compound protease into the degreased corn stigma powder, soaking, carrying out primary heating extraction, cooling, then adding bromelain and the solution A, soaking, carrying out secondary heating extraction to obtain a leaching solution, and removing precipitates after enzyme deactivation and centrifugation to obtain a corn stigma polysaccharide clear solution; (3) impurity removal: dialyzing the corn stigma polysaccharide clear liquid to obtain dialyzed internal liquid; and (4) post-treatment: washing and drying the dialysis internal liquid to obtain the corn stigma polysaccharide. High-yield and high-purity corn stigma polysaccharide extraction can be realized, and the corn stigma polysaccharide can be applied to hypoglycemic products.

Description

technical field [0001] The invention relates to the technical field of extraction, in particular to a preparation method of corn silk polysaccharide and its application in hypoglycemic products. Background technique [0002] Corn silk is the style and stigma of the gramineous plant maize. It has the functions of diuresis and swelling, clearing the liver and gallbladder, and has a wide range of sources, low price, and easy collection. It has a relatively broad research prospect. Corn silk polysaccharide is one of the main active components of corn silk, accounting for about 4.87% of the dry weight of corn silk, and the specific types include glucose, arabinose, galactose and mannose. Literature: Zhang Jiajia, He Zhitao, Wang Xinru, et al. Research progress on the pharmacological effects of corn silk polysaccharides [J]. Journal of Jilin University of Medicine, 2021, 42(1): 3. et al. recorded the effects of corn silk polysaccharides in anti-tumor, hypoglycemic, regulating The...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00A61K31/715A61P3/10
CPCC08B37/0003A61K31/715A61P3/10
Inventor 殷金龙祝新雷鞠凤霞袁湘张小丽张远东董志杰黄丙昌许洪成
Owner JILIN BUSINESS & TECH COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap