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High-grafting active lignin as well as preparation method and application thereof

A lignin and high-grafting technology, applied in the direction of botany equipment and methods, applications, chemical instruments and methods, etc., can solve the problems of DNA damage to human immunity, induction of uterine fibroids, lung cancer, and lack of lignin particles , to achieve the effect of improving antioxidant capacity, reducing oxidative damage, and improving UV shielding ability

Active Publication Date: 2022-06-28
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The literature (Nutrients, 2021, 13(9) and Environmental Science&Technology, 2020, 54(19): 11706-19) pointed out that the use of synthetic antioxidants, such as butylated hydroxytoluene (BHT) and tert-butyl p-hydroxyanisole ( When BHA) is used as a food preservative, there is a risk of causing DNA damage and a decline in human immunity. In severe cases, it may even induce uterine fibroids and lung cancer. Some natural antioxidants are replacing synthetic antioxidants
As one of the natural antioxidants, there is no relevant literature report on the use of lignin particles in fruit and vegetable preservation in China.

Method used

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  • High-grafting active lignin as well as preparation method and application thereof
  • High-grafting active lignin as well as preparation method and application thereof
  • High-grafting active lignin as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A preparation method of high grafting activity lignin, the steps are as follows:

[0065] (1) Preparation of raw materials: in parts by weight, 100 parts of lignin, 2 parts of sodium hydroxide, 4 parts of urea, 800 parts of water, 2 parts of dilute hydrochloric acid with pH 2, 2 parts of laccase, and lemon with pH 2 800 parts of acid-sodium citrate buffer;

[0066] (2) Dissolving lignin in a sodium hydroxide / urea / water mixed solution at -20°C for 20 h to obtain a lignin mixed solution;

[0067] (3) add dilute hydrochloric acid dropwise to the lignin mixed solution obtained in step (2) at a speed of every drop (each drop is 0.6 ml) for three seconds to separate out lignin particles, and wash the lignin particles for 4 times. Dehydration after washing;

[0068] (4) putting the lignin particles washed and dehydrated in step (3) into a citric acid-sodium citrate buffer, adding laccase, and stirring at a rate of 800 r / min at 10° C. for 20 hours;

[0069] (5) washing and d...

Embodiment 2

[0073] A preparation method of high grafting activity lignin, the steps are as follows:

[0074] (1) Preparation of raw materials: in parts by weight, 100 parts of lignin, 6 parts of sodium hydroxide, 6 parts of urea, 1000 parts of water, 4 parts of dilute hydrochloric acid with pH 3, 20 parts of laccase, and lemon with pH 4 2000 parts of acid-sodium citrate buffer;

[0075] (2) Dissolving lignin in a sodium hydroxide / urea / water mixed solution at -10°C for 30 h to obtain a lignin mixed solution;

[0076] (3) add dilute hydrochloric acid dropwise to the lignin mixed solution obtained in step (2) at a speed of every drop (each drop is 1 ml) for five seconds to separate out lignin particles, and wash the lignin particles for 6 times, each time Dehydration after washing;

[0077] (4) putting the lignin particles washed and dehydrated in step (3) into a citric acid-sodium citrate buffer, adding laccase, and stirring at a rate of 1000 r / min at 35° C. for 15 h;

[0078] (5) washin...

Embodiment 3

[0082] A preparation method of high grafting activity lignin, the steps are as follows:

[0083] (1) Preparation of raw materials: in parts by weight, 100 parts of lignin, 8 parts of sodium hydroxide, 8 parts of urea, 1200 parts of water, 4 parts of dilute hydrochloric acid with pH 2, 8 parts of laccase, and acetic acid with pH 6 - 1000 parts of sodium acetate buffer;

[0084] (2) Dissolving lignin in a sodium hydroxide / urea / water mixed solution at 20°C for 12 h to obtain a lignin mixed solution;

[0085] (3) in the lignin mixed solution obtained in step (2), add dilute hydrochloric acid dropwise at a rate of every drop (each drop is 0.6 ml) for three seconds to separate out lignin particles, and wash the lignin particles for 6 times. Dehydration after washing;

[0086] (4) put the lignin particles washed and dehydrated in step (3) into a buffer solution, add laccase, and stir at a rate of 600 r / min at 50° C. for 12 h;

[0087] (5) washing and dehydrating the product of ste...

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Abstract

The invention relates to highly grafted active lignin as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving lignin in a sodium hydroxide / urea / water mixed solution at a certain temperature to obtain a lignin mixed solution; dilute hydrochloric acid is dropwise added into the lignin mixed solution, lignin particles are separated out, and the lignin particles are washed with water for multiple times; putting the washed and dehydrated lignin particles into a buffer solution, adding laccase, and stirring for a period of time at a certain temperature; and finally, washing the product with water for multiple times, and drying to obtain the high-grafting active lignin. The phenolic hydroxyl group content of the prepared high-grafting active lignin is increased by 30-60% compared with that before the reaction; and the DPPH free radical scavenging rate and the PTIO free radical scavenging rate of the highly grafted active lignin are respectively 70-95% and 50-80%. The treatment mode is low in cost, green and environment-friendly, the high yield is achieved, and the high-grafting-activity lignin can be applied to the fields of fruit and vegetable preservatives, anti-ultraviolet and anti-oxidation additives, food preservatives, antibacterial agents and the like.

Description

technical field [0001] The invention belongs to the technical field of natural polymer-based materials, and relates to a high-grafting activity lignin and its preparation method and application, in particular to a high-grafting activity lignin and its preparation method and its application in fruit and vegetable preservation, anti-oxidation, and anti-oxidation. UV and antibacterial applications. Background technique [0002] In 1838, when French chemist and botanist P.Payen separated cellulose from wood, he discovered that there was a substance that was always associated with cellulose and hemicellulose, and had a higher carbon content. He called this substance " La matiere LigneuseVertable" (French, real woody substance). Lignin, cellulose, and hemicellulose constitute the plant skeleton. Lignin is extremely abundant in nature, and 150 billion tons of lignin can be produced globally through photosynthesis every year. In terms of total amount, lignin is the third most abu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H7/00C12P1/00A23B7/154A01N61/00A01P1/00C09K15/34
CPCC08H6/00C12P1/00A23B7/154A01N61/00C09K15/34
Inventor 毛志平郁海楠王雅梅阮玉龙武守营吴伟王碧佳冯雪凌钟毅徐红纪柏林徐有琦邹峰
Owner DONGHUA UNIV
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